The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary reagents for cell lysis and luciferase quantitation.Sensitive: highly sensitive detection of firefly luciferase activity.Stable: the signal is stable for more than two hours, providing flexibility regarding incubation time.Convenient: simple one-step, homogeneous protocol.