PAN-Biotech William's Medium E, w: stable Glutamine, w: 2.24 g/L NaHCO3 (500 ml). BICARBONATE: With 2.24 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: With Stable Glutamine. PHENOL RED: With Phenol Red. HEPES: Without HEPES. SODIUM PYRUVATE: With sodium pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: Williams and Gunn originally developed William's E Medium as a reduced serum-supplemented medium for long-term cell cultures of adult rat liver epithelial cells. William's E Medium may also be used for the cultivation of primary hepatocyte cells of other species as well as human-derived HepaRG cells. The medium is unique in that it contains zinc, iron, manganese, non-essential amino acids, the reducing agent glutathione, and the lipid methyl linoleate.