General Cell Lines and Cell Cultures
Eurofins Discoverx PrecisION hHCN3 Recombinant Stable Cell Line, 2 vials. (CYL3042)
The PrecisION hHCN3 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying HCN3 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses HCN3 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
Eurofins Discoverx PathHunter CHO-K1 SSTR4 β-Arrestin Cell Line, 2 vials. (93-0308C2)
The PathHunter SSTR4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. somatostatin) based activation of SSTR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged SSTR4 and EA-tagged β-Arrestin-2. Activation of SSTR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx Ready-to-Assay LPA5/GPR92 Lysophospholipid Receptor Frozen Cells, 2 Vials of Cells. (HTS209RTA)
The Ready-to-Assay LPA5/GPR92 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. LPA) based activation of LPAR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses LPAR5 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of LPAR5, the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. For detailed product information, User manual and datasheet, visit www.discoverx.com/products-applications/frozen-cells.
ATCC Pfizer Pneumococcal polysaccharide powder Type 68 (d9V) 2mg
Purified pneumococcal polysaccharide type 9V is manufactured by Pfizer, Inc. for small quantity distribution by ATCC. The pneumococcal powder type 9V is a component of the current 13-valent vaccine against Streptococcus pneumoniae. The pneumococcal polysaccharide type 9V can be used to assay for type-specific antibodies, research immune responses and study immunodeficiencies.
ABclonal Technology HRH4 Rabbit pAb
Histamine is a ubiquitous messenger molecule released from mast cells, enterochromaffin-like cells, and neurons. Its various actions are mediated by a family of histamine receptors, which are a subset of the G-protein coupled receptor superfamily. This gene encodes a histamine receptor that is predominantly expressed in haematopoietic cells. The protein is thought to play a role in inflammation and allergy reponses. Multiple transcript variants encoding different isoforms have been found for this gene.
Eurofins Discoverx ChemiScreen β3 Adrenoceptor Receptor Membrane Preparation, 1 vial. (HTS159M)
The ChemiScreen β3 Adrenoceptor Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of ADRB3 surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Eurofins Discoverx ChemiScreen BLT1 Leukotriene Receptor Stable Cell Line, 2 vials. (HTS042C)
The ChemiScreen BLT1 Leukotriene Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene) based activation of LTB4R GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses LTB4R alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of LTB4R, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Amsbio LLC Normal Human Normal Human Intrahepatic Biliary Epithelial Cells /Cholagioctyes/, passage 2
Normal Human Normal Human Intrahepatic Biliary Epithelial Cells /Cholagioctyes/, passage 2
TRIBIOSCIENCE INC Malondialdehyde (MDA) Colorimetric Assay Kit 100 Assays
The MDA Assay provides a sensitive, simple and accurate approach to determine MDA concentration in tissue, cells and other biosamples. APPLICATION Direct Assays - Quantitative detection of MDA in cell, tissue, plasma, serum, and urine samples. KEY FEATURES High-Throughput - Designed for 96-well plates, allowing multiple samples to be tested at the same time. Simple and Rapid Procedure - The test process is straightforward and takes about 60 minutes to complete.
Eurofins Discoverx PathHunter CHO-K1NR β-Arrestin Cell Line, 2 vials. (93-0323C2)
The PathHunter MLNR cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. motilin) based activation of MLNR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MLNR and EA-tagged β-Arrestin-2. Activation of MLNR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Biochain Institute Inc Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel II, 8 tissue spots, 5 slides/PK
BioChain's FFPE multiple tissue panels with different types of tumors are designed for screening gene expression in multiple tumor tissues. Each tumor tissue is paired with a corresponding normal control tissue. A licensed pathologist issued a pathological diagnosis of each tumor based on examination of tissue sections. Documentation of tissues' clinical histories is available upon request. mRNA Northern blot and Western blots as well as total RNA Norterhn blot are available in identical formats.
STEMCELL Technologies G-CSF and Plerixafor Mobilized Human Peripheral Blood Mononuclear Cells, Frozen, Size: 5 x 10^7 cells
Start your assays with ready-to-use, ethically sourced, mobilized primary mononuclear cells. We help you get the cells you need with personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications. Donors are mobilized with G-CSF, plerixafor, or G-CSF and plerixafor prior to collection. Isolated from leukapheresis samples using density gradient separation or red blood cell lysis and cryopreserved in CryoStorCS10 medium, cells are obtained using Institutional Review Board (IRB)-approved consent forms and protocols. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. ACDA is added as an anticoagulant. Contact techsupport@stemcell.com for donor specifications (BMI category, smoking status, ethnicity, etc.). Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Certain products are only available in select territories.
Eurofins Discoverx PathHunter CCR5 Activated GPCR Internalization U2OS Cell Line, 2 vials. (93-0653C3)
The PathHunter CCR5 Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of CCR5 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CCR5. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CCR5-arrestin complex is internalized to the early endosome, leading to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Cayman Chemical 9-cIs RetInal 10mg
A natural retinoid that is produced by oxidation of 9-cis retinol by cRDH; binds to CRBP-I, CRBP-II, and CRALBP (Kds = 8, 5, and 53.3 nM, respectively); inhibits differentiation of RCJ C5.18 chondrogenic cells into cartilage in vitro (IC50 = 8 nM); it rescues cone ERG responses in Irbp-/- mice (0.375 mg)
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Heart, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.