General Cell Lines and Cell Cultures
Eurofins Discoverx CHO-K1 ADRA1A Gq Cell Line, 2 vials. (95-1023C2)
The ADRA1A Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA1A, a naturally Gq coupled GPCR. Upon activation of ADRA1A, the resulting calcium activity can be fluorescently assayed using the Calcium No WashPLUS Detection Kit. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Promocell GmbH PromoCell HTSMC Pellet from > 1 million Normal Human Tracheal Smooth Muscle Cells
Cell pellet consisting of 1 million cells dissolved in 200µl RNAlater for subsequent RNA, DNA or protein analysis. Note: Cell pellets cannot be revived.Our Cell Pellets are stored in RNAlater – an aqueous protection solution that rapidly permeates the cells and stabilizes cellular DNA, RNA, and proteins and protects them from degradation. This makes our Cell Pellets very useful for a wide range of research applications like gene expression analysis, cell characterization studies, and protein characterization experiments.Each Cell Pellet is made from 1 million cells and is dissolved in 200 µl RNAlater. The Cell Pellets are produced from released cell lots and have the same characteristics, i.e. expression profile or cell type specific antigens. On request, viable cells from the same lot are available for cultivation.
ATCC Cryptococcus gattii; RV 48370
An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
ATCC Mycoplasma felis
This strain of Mycoplasma felis was isolated from the eye of a cat with conjunctivitis. This culture is a bacterial type strain and is propagated in a 5% CO2 atmosphere on Mycoplasma Medium.
Quantified Genomic DNA; from Escherichia coli; strain HMS174; with ATCC 16S Tag 1
Quantitative genomic DNA from Escherichia coli with ATCC 16S Tag 1 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load. The synthetic Tag 1 allows for use as a spike-in control in assay development, library preparation, quality control, and data normalization for both 16S rRNA and shotgun metagenomics approaches. This product is also a component of MSA-1014.
ATCC pRS426 phagemid; in E. coli
This is a YE-type shuttle vector encoding the beta-galactosidase alpha peptide, permitting blue-white visual detection. It can be used to produce ssDNA and it contains promoters for in vitro RNA synthesis and priming sites useful for sequencing. It also contains the REP3 and FRT sequences necessary for high copy propagation in yeast. In S. cerevisiae, the copy number is about 20 per haploid cell. In non-selective growth, plasmids are lost through mitotic segregation at rates in the range of 4.4 +/- 1.4% of progeny per doubling.
ATCC Clostridium tyrobutyricum
Clostridium tyrobutyricum strain VPI 5392 is a whole-genome sequenced bacterial type strain.
ATCC Burkholderia thailandensis; Strain E264; genomic DNA
Genomic DNA isolated from Burkholderia thailandensis strain E264. This bacterial strain is also available as ATCC® Catalog No. 700388™.
