Cell Based Assays
Promega ROCK2 Kinase Enzyme System, 1mg
ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element.
Promega PRKG1 (PKG) Kinase Enzyme System, 1mg
Recombinant full-length human PRKG1 (PKG) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PRKG1 is a homodimer, with each monomer containing a regulatory cGMP-binding domain and a catalytic domain.
Promega ADP-Glo™ Kinase Assay + p38γ Kinase Enzyme System
Recombinant full-length human p38 gamma was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38 gamma is a member of the p38 MAPK family, which is activated in response to stress.
Promega ADP-Glo™ Kinase Assay + IRAK4 Kinase Enzyme System
Recombinant full-length human IRAK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Interleukin-1 receptor-associated kinase 4 is an important mediator in the signal transduction of Toll-like receptor and IL1R family members.
Molecular Probes™ Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry
Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry
Molecular Probes™ pHrodo™ Green E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry
pHrodo™ Green E. coli BioParticles™ Phagocytosis Kit for Flow Cytometry
Promega NanoBiT PPI MCS Starter System, 1 each
The NanoBiT PPI Starter Systems provide the vectors required to create the LgBiT and SmBiT protein fusions, a PRKACA:PRKAR2A positive control pair and a negative control vector. Starter systems also include the Nano-Glo Live Cell Assay System.
LanthaScreen™ TR-FRET ER alpha Competitive Binding Kit
LanthaScreen™ TR-FRET ER alpha Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) alpha.
Promega Corporation 4-1BB Bioassay 5X
Use a simpler, more consistent 4-1BB Bioassay for a mechanism-of-action (MOA)-based assay that is prequalified according to ICH guidelines. No primary cell culture required. Supplied in a convenient thaw-and-use format, also available in cell propagation model (CPM) format.
Promega NanoBRET TE Intracellular BET BRD Assay, 1,000 assays
NanoBRET Target Engagement Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. The assay is based on NanoBRET, an energy transfer technique for measuring molecular proximity in living cells.
Promega ADP-Glo™ Kinase Assay + PIM2 Kinase Enzyme System
Recombinant full-length human PIM2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PIM2 is a serine threonine kinase that is present in all tissues.
Promega ADP-Glo™ Kinase Assay + ZAK Kinase Enzyme System
Recombinant full-length human ZAK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAK is a member of the MAPKKK family of signal transduction molecules and mediates gamma radiation signaling leading to cell cycle arrest.
Promega ADP-Glo™ Kinase Assay + NUAK2 Kinase Enzyme System
Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2.
Promega ADP-Glo™ Kinase Assay + PLK1 Kinase Enzyme System
Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. A member of the Polo-Like Kinase family, PLK1 localizes to centrosomes and undergoes dramatic subcellular relocation during the cell cycle.