Signal Transduction Reagents and Kits
Promega p38 beta Kinase Enzyme System, 1mg
Recombinant full-length human p38 beta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38 beta is a member of the p38 MAP kinase family and is activated by both proinflammatory cytokines and environmental stress.
Promega MAPKAPK3 Kinase Enzyme System, 1mg
Recombinant full-length human MAPKAPK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK3 is closely related to MAPKAPK2 sharing 72% nucleotide and 75% amino acid identity.
Promega MAPKAPK5 Kinase Enzyme System, 1mg
Recombinant full-length human MAPKAPK5 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MAPKAPK5 is a member of the serine/threonine kinase family that responds to cellular stress and proinflammatory cytokines.
Promega MLK2 Kinase Enzyme System, 1mg
Recombinant human MLK2 (1-446) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK2 or mixed-lineage kinase 2 is a member of the serine/threonine kinase family, which activates MAPK8/JNK1 and MKK4/SEK1.
Promega MST1 Kinase Enzyme System, 1mg
Recombinant full-length human MST1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MST1 belongs to a family of proteins that share similarity with a budding yeast serine/threonine kinase, sterile-20 (Ste20).
Promega NIK Kinase Enzyme System, 1mg
Recombinant human NIK (325-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NIK is a mitogen-activated protein kinase kinase kinase 14 (MAP3K14), which binds to TRAF2 and stimulates NF-kappaB activity.
Promega MAPKAPK2 Kinase Enzyme System, 1mg
Recombinant human MAPKAPK2 (46-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. MAPKAPK2 (MAPKAP kinase2) is a Ser/Thr protein kinase that is regulated via direct phosphorylation by p38 MAP kinase.
Promega PAK1/CDC42 Kinase Enzyme System, 1mg
Recombinant full-length human PAK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag and combined with CDC42 at a ratio of 1:4 (PAK1:CDC42) in vitro. PAK1 is a member of the p21-activated kinases (PAKs).
Promega TAK1-TAB1 Kinase Enzyme System, 1mg
Recombinant human proteins TAK1 (1-303) and TAB1 (437-end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ABL1 (E255K) Kinase Enzyme System, 1mg
Recombinant human ABL1 (E255K) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega SLK Kinase Enzyme System, 1mg
Recombinant full-length human SLK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SLK or LOSK is a Ste20-like protein kinase that associates with microtubule and centrosome.
Promega ABL1 (Y253F) Kinase Enzyme System, 1mg
Recombinant human ABL1 (Y253F) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay with PIP2:3PS
The ADP-Glo™ Kinase assay and lipid kinase substrates PIP2:3PS and PI:3PS are components of the PI3K-Glo™ Class I Kinase Profiling System that are available for purchase separately.
Promega CAMKK1 Kinase Enzyme System, 1mg
CAMKK1 or CAMKK alpha is a Ca2+/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively.
Promega AMPK (A1/B1/G1) Kinase Enzyme System, 1mg
Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags. AMPK is an important energy-sensing enzyme that monitors cellular energy status.