Signal Transduction Reagents and Kits
Promega ABL1 Kinase Enzyme System, 1mg
Recombinant human ABL1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. ABL1 proto-oncogene encodes a cytoplasmic and nuclear protein tyrosine kinase implicated in processes of cell differentiation, cell division and others.
Promega ADP-Glo™ Kinase Assay + RET (Y791F) Kinase Enzyme System
Recombinant human RET (Y791F) (amino acids 658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega CLK1 Kinase Enzyme System, 1mg
Recombinant human CLK1 (129-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CLK1, also known as STY, is a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases.
Promega AMPK (A2/B1/G1) Kinase Enzyme System, 10microg
Recombinant full-length human AMPK (A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for diabetes treatment.
Promega ADP-Glo™ Kinase Assay + AMPK (A1/B1/G2) Kinase Enzyme System
Recombinant full-length human AMPK (combination of A1/B1/G2 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMP-activated protein kinase (AMPK) exhibits a key role as master regulator of cellular energy homeostasis.
Promega ADP-Glo™ Kinase Assay + CAMK2α Kinase Enzyme System
Recombinant full-length human CAMK2 alpha was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 alpha is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family.
Promega ADP-Glo™ Kinase Assay + PYK2 Kinase Enzyme System
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega EPHA1 Kinase Enzyme System, 1mg
Recombinant mouse EPHA1 (amino acids 569-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EPHA1 is a member of the Eph family of receptor tyrosine kinases that have been implicated in mediating developmental events.
Promega ADP-Glo™ Kinase Assay + ERK1 Kinase Enzyme System
Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega ADP-Glo™ Kinase Assay + JNK3 Kinase Enzyme System
Recombinant full-length human JNK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JNK3 is a member of the c-Jun N-terminal kinases (JNKs), which are part of the mitogen-activated protein (MAP) kinase family.
Promega NEK2 Kinase Enzyme System, 10microg
Recombinant full-length human NEK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans.
Promega ASK1 Kinase Enzyme System, 1mg
Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death.
Promega GRK5 Kinase Enzyme System, 1mg
Recombinant full-length human GRK5 was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. GRK5 is a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family.
Promega ULK1 Kinase Enzyme System, 1mg
Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ULK1 is a serine/threonine protein kinase that plays a critical role during initial stages of autophagy, a vital response to nutrient starvation.
Promega CAMK2 alpha Kinase Enzyme System, 1mg
Recombinant full-length human CAMK2 alpha was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. CAMK2 alpha is a ser/thr protein kinase that is a member of the Ca2+/calmodulin-dependent protein kinase family.