Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + PAK3 Kinase Enzyme System
Recombinant full-length mouse PAK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PAK3 is a member of the family of ser/thr p21-activating kinases that serve as targets for the small GTP-binding proteins Cdc42 and RAC.
Promega ADP-Glo™ Kinase Assay + TAOK1 Kinase Enzyme System
TAOK1 is a serine/threonine-protein kinase involved in regulation of the p38-containing stress-responsive MAP kinase pathway and extracellular signal-regulated protein kinase (ERK) kinases (MEKs).
Promega ADP-Glo™ Kinase Assay + PKA Kinase Enzyme System
Recombinant bovine cAMP-Dependent Protein Kinase (PKA), Catalytic Subunit, is expressed and purified from E. coli. PKA does not require cAMP for activity, and the gene sequence can be found at accession number NM_174584.
Promega CAMKK1 Kinase Enzyme System, 10microg
CAMKK1 or CAMKK alpha is a Ca2+/calmodulin-dependent protein kinase that activates CAM-kinases I and IV via phosphorylation of their Thr(177) and Thr(196) residues, respectively.
Promega CDK3/CyclinE1 Kinase Enzyme System, 10microg
Recombinant full-length human CDK3 and Cyclin E1 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK3/CyclinE1 is a member of the cyclin-dependent protein kinase family.
Promega NEK3 Kinase Enzyme System, 10microg
Recombinant full-length human NEK3 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK3 is a member of the NEK family of protein kinases that share high amino acid homology with NIMA (never in mitosis gene a).
Promega CDK6/CyclinD3 Kinase Enzyme System, 10microg
Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Promega ABL1 (E255K) Kinase Enzyme System, 10microg
Recombinant human ABL1 (E255K) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + CK2α1 Kinase Enzyme System
Recombinant full-length human CK2 alpha 1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CK2 alpha 1 is a serine-threonine protein kinase whose targets include many critical regulators of cellular growth.
Promega ADP-Glo™ Kinase Assay + ALK2 Kinase Enzyme System
ALK2 is a receptor serine/threonine kinase that is member of the ALK family and is upstream of signaling pathway involving the SMAD proteins especially SMAD1/5/8.
Promega c-KIT Kinase Enzyme System, 10microg
Recombinant human c-KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
Promega ALK4 Kinase Enzyme System, 10microg
Recombinant human ALK4 (150-end) was expressed in Sf9 cells using an N-terminal GST tag. ALK4 is a member of the subfamily of receptor ser/thr kinases that mediates signaling by the Activins.
Promega ADP-Glo™ Kinase Assay + MSK2 Kinase Enzyme System
Recombinant full-length mouse MSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ABL1 (Y253F) Kinase Enzyme System, 10microg
Recombinant human ABL1 (Y253F) (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + DYRK2 Kinase Enzyme System
Recombinant full-length human DYRK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.