Signal Transduction Reagents and Kits
Promega ADP-Glo™ Kinase Assay + EIF2AK2 Kinase Enzyme System
Recombinant human EIF2AK2 (252-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EIF2AK2 (also known as double-stranded RNA-activated protein kinase) is shown to be involved in HIV/gp120-associated neurodegeneration.
Promega ROCK2 Kinase Enzyme System, 1mg
ROCK2 is a ubiquitously expressed serine/threonine kinase localized in the nucleus that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element.
Promega PASK Kinase Enzyme System, 10microg
Recombinant human PASK (981-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PASK or PAS domain containing serine/threonine kinase regulates the function of many intracellular signaling pathways involved in stress.
Promega cAMP-Glo™ Assay
The cAMP-Glo™ Assay is a homogeneous, bioluminescent and high-throughput assay for measuring cAMP levels in cells
Promega IKK alpha Kinase Enzyme System, 10microg
Full-length recombinant human IKK alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKK alpha is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein.
Promega ADP-Glo™ Kinase Assay + c-KIT Kinase Enzyme System
Recombinant human c-KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
Promega IRAK4 Kinase Enzyme System, 10microg
Recombinant full-length human IRAK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Interleukin-1 receptor-associated kinase 4 is an important mediator in the signal transduction of Toll-like receptor and IL1R family members.
Promega Luciferin Detection Reagent
A reagent to detect presence of pro-luciferin substrates used to monitor the activity of specific isoforms of cytochrome P450 or NAT2.
Promega ADP-Glo™ Kinase Assay + IRAK4 Kinase Enzyme System
Recombinant full-length human IRAK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Interleukin-1 receptor-associated kinase 4 is an important mediator in the signal transduction of Toll-like receptor and IL1R family members.
Promega ADP-Glo™ Kinase Assay + CDK5/p25 Kinase Enzyme System
Recombinant full-length human CDK5 and p25 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.
Promega Glutamine/Glutamate-Glo Assay, 5ml
The Glutamine/Glutamate-Glo Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples. The assay is based on the conversion of glutamine to glutamate by Glutaminase enzyme.
Promega p38 alpha Kinase Enzyme System, 1mg
Recombinant full-length human p38alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. p38alpha (SAPK2A) is a member of the p38 MAPK family, which are activated by various environmental stresses and proinflammatory cytokines.
Promega ADP-Glo Kinase Assay + Aurora A Kinase Enzyme System, 1 each
Recombinant full-length human Aurora A was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. Aurora A belongs to a multigenic family of mitotic serine/threonine kinases, which are involved in the control of chromosome segregation.
Promega ADP-Glo™ Kinase Assay with PIP2:3PS
The ADP-Glo™ Kinase assay and lipid kinase substrates PIP2:3PS and PI:3PS are components of the PI3K-Glo™ Class I Kinase Profiling System that are available for purchase separately.
Promega ADP-Glo™ Kinase Assay + PASK Kinase Enzyme System
Recombinant human PASK (981-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PASK or PAS domain containing serine/threonine kinase regulates the function of many intracellular signaling pathways involved in stress.