Signal Transduction Reagents and Kits
Promega IKK beta Kinase Enzyme System, 10microg
Full length recombinant human IKKβ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega KHS1 Kinase Enzyme System, 10microg
Recombinant full-length human KHS1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. KHS1 belongs to the ser/thr kinase family that has a STE20-like protein kinase domain, which stimulates the SAPK pathway.
Promega PLK1 Kinase Enzyme System, 1mg
Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. A member of the Polo-Like Kinase family, PLK1 localizes to centrosomes and undergoes dramatic subcellular relocation during the cell cycle.
Promega MLK1 Kinase Enzyme System, 1mg
Recombinant human MLK1 (1-433) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Mixed-Lineage Kinase 1 is a mitogen-activated protein kinase kinase kinase capable of activating the c-Jun NH(2)-terminal kinase (JNK) pathway.
Promega ADP-Glo™ Kinase Assay + MSK1 Kinase Enzyme System
Recombinant full-length human MSK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega AMPK (A2/B1/G1) Kinase Enzyme System, 10microg
Recombinant full-length human AMPK (A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for diabetes treatment.
Promega ADP-Glo™ Kinase Assay + FES Kinase Enzyme System
Recombinant full-length human FES was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FES is a proto-oncogene that encodes a protein-tyrosine kinase distinct from c-Src, c-Abl and other nonreceptor tyrosine kinases.
Promega ULK1 Kinase Enzyme System, 1mg
Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ULK1 is a serine/threonine protein kinase that plays a critical role during initial stages of autophagy, a vital response to nutrient starvation.
Promega ADP-Glo™ Kinase Assay + TOPK Kinase Enzyme System
Recombinant full-length human TOPK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TOPK is a MAPK kinase that phosphorylates p38 MAPK and is activated in a cell-cycle-dependent manner in neuronal progenitor cells in vitro.
Promega CSK Kinase Enzyme System, 1mg
Recombinant full-length human CSK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CSK has been shown to downregulate the tyrosine kinase activity of c-src through tyrosine phosphorylation of the c-src carboxy terminus.
Promega PI3K (p120gamma), 20ug, 200microl
The PI3K lipid kinase enzymes are used with ADP-Glo KInase Assay System and Lipid Kinase Substrates in a homogeneous, add-mix-read lipid kinase assay.
Promega PIP2:3PS Lipid Kinase Substrate, 0.25mg, 0.25ml
The lipid kinase substrates PIP2:3PS and PI:3PS are standalone substrates that are also part of the PI3K-Glo kinase profiling system.
Promega PKD2 Kinase Enzyme System, 10microg
Recombinant full-length human PKD2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PKD2 is a novel phorbol ester- and growth factor-stimulated serine/threonine kinase.
Promega ADP-Glo™ Kinase Assay + FGFR3 (K650E) Kinase Enzyme System
Recombinant human FGFR3 (K650E) (397-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Fibroblast growth factor receptor 3 is part of a family of fibroblast growth factor receptors that share similar structure and function
Promega ADP-Glo™ Kinase Assay + PLK1 Kinase Enzyme System
Recombinant full-length human PLK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. A member of the Polo-Like Kinase family, PLK1 localizes to centrosomes and undergoes dramatic subcellular relocation during the cell cycle.