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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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Peptide N-glycosidase F (PNGase F) is a glycosylpeptidase with a theoretical molecular weight of 35.9 kD that can cleave asparagine-linked high mannose, hybrid, and complex oligosaccharides from glycoproteins. PNGase F cleaves the amide bond between innermost N-acetylglucosamine (GlcNAc) and asparagine residues of the glycoprotein, converting asparagine to aspartic acid in the process. This product is commonly used for the deglycosylation of antibodies and glycoproteins.
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A high fidelity restriction endonuclease that recognizes the sequence GTA^TAC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
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A high fidelity restriction endonuclease that recognizes the sequence T^GATCA. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
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Deoxyribonuclease I, Chromatographically purified, A lyophilized powder containing glycine as a stabilizer, Contains =0.005% Rnase, Store at 2 to 8degreeC, Pack Size: 5mg, Source: Bovine Pancreas, Minimum Activity: > /=2000 Kunitz U/mg dry
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Qiagen Proteinase K (10) for protease digestion during DNA and RNA preparation. Features/benefits Broad substrate specifity High activity for a range of conditions Compatible with most common DNA and RNA isolation buffers. Contents 10m
Carboxypeptidase-B Rat Recombinant, Carboxypeptidase B Rat,Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 35kDa is purified by standard chromatography techniques.
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Endoproteinase LysC is a serine Endoproteinase which cleaves peptide bonds at the carboxyl side of lysine. LysC is a sequencing grade enzyme and is suitable for proteomics and glycobiology applications.
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Exonuclease VII (Exo VII) can cleave single-stranded DNA in both 5-3 and 3-5 directions and is inactive against linear or circular double-stranded DNA. When PCR requires stepwise completion to obtain the final target product, this enzyme can be used to remove residual oligonucleotide primers left during intermediate stages of PCR, eliminating interference with subsequent use of different primers. The enzymes digestion of single-stranded DNA is independent of metal ions. This product is a highly purified protein obtained through the recombinant expression of the Exonuclease VII gene (XseA and XseB).
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