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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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Endoproteinase LysC is a serine Endoproteinase which cleaves peptide bonds at the carboxyl side of lysine. LysC is a sequencing grade enzyme and is suitable for proteomics and glycobiology applications.
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UBE1 is an E1 enzyme within the E1, E2, E3 cascade which conjugates Ub to protein substrates. UBE1 activates Ub by catalyzing an ATP – dependent reaction; Ub is conjugated onto the catalytic cysteine residue of UBE1 by formation of a thioester bond. The activated Ub is then passed on to a Ub-conjugating enzyme E2; Ub charged E2 binds a Ub ligase E3 that directly binds substrate proteins. Ub is transferred to the HECT domain E3s prior to being conjugated on substrate proteins. For RING domain E3s, Ub is transferred directly from an E2 to substrate proteins.
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Dabcyl-KTSAVLQSGFRKME-Edans is an internally quenched fluorogenic substrate for the SARS-CoV family 3C-like protease (3CLP, also called main protease or MPro) with a Km value of 17 uM. Upon 3CLP-mediated cleavage between glutamine and serine, the resulting SGFRKME-EDANS fluorescence can be monitored at the excitation/emission wavelengths of 355/538 nm, respectively.
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