Enzymes and Inhibitors
New England Biolabs, Inc. EnGen Lba Cas12a (Cpf1) – 70 pmol
EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) . Targeting requires a gRNA complementary to the target site as well as a 5' TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a occurs ~18 bases 3' of the PAM and leaves 5' overhanging ends. EnGen Lba Cas12a has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
New England Biolabs, Inc. NEBuffer Set (EcoRI, DpnII) – 1.25 ml of each
New England Biolabs provides 10X NEBuffer with each restriction endonuclease to assure optimal (100%) activity. Set contains two 1.25ml vials of each unique NEBuffer for the enzymes EcoRI and DpnII. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. 1X NEBuffer EcoRI 100 mM Tris-HCl 50 mM NaCl 10 mM MgCl2 0.025% Triton X-100 (pH 7.5 @ 25C) 1X NEBuffer DpnII 50 mM Bis-Tris-HCl 100 mM NaCl 10 mM MgCl2 1 mM DTT (pH 6 @ 25C)
New England Biolabs, Inc. Esp3I – 300 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN_....
New England Biolabs, Inc. AleI-v2 – 2500 units
AleI-v2 is an engineered and improved version of AleI that recognizes the sequence CACNN^NNGTG.
New England Biolabs, Inc. Template Switching RT Enzyme Mix – 100 reactions
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template. These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.
New England Biolabs, Inc. Nuclease P1 – 10000 units
Nuclease P1 (from P. citrinum) is a zinc-dependent single-strand specific nuclease which hydrolyzes 3->5 phosphodiester bonds in RNA and ssDNA with no base specificity. Nuclease P1 also exhibits 3-phosphomonoesterase activity. Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer r1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.
Enzyme Research Laboratories Inc HUMAN FIBRINOGEN 1G
Human Fibrinogen that is plasminogen, vWF and Fibronectin depleted.
Diapharma Group, Inc. Chromogenix S-2251™, Chromogenic Substrate For Plasmin Detection, Reliable For Fibrinolysis Studies, 25 mg
Chromogenix S-2251 is a specialized chromogenic substrate designed for the accurate detection and quantification of plasmin and streptokinase-activated plasminogen in plasma. It provides reliable results for hemostasis and fibrinolysis studies, making it essential for researchers in these fields. Its unique chromogenic method enhances detection, ensuring elevated reproducibility and precision in assays. This product is determined by its high-quality formulation and extensive application range.
Sigma Aldrich Fine Chemicals Biosciences Pectinase Aspergillus250ML
We are committed to bringing youAGreenerAAlternative Products which adhere to one or more of The 12 Principles ofAGreenerAChemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. for more information see the article in biofiles and Enzymes for Alternative Energy Research.
Sigma Aldrich Fine Chemicals Biosciences Glutathione Reductase from baker's yeast (S. cerevisiae) ammonium sulfate suspension, 100-300 units/mg protein (biuret) | 9001-48-3 | MFCD00131196 | 100UN
Glutathione Reductase from baker's yeast (S. cerevisiae) ammonium sulfate suspension, 100-300 units/mg protein (biuret) | 9001-48-3 | MFCD00131196 | 100UN
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium album, 39450-01-6, MFCD00132129, 25 mg
Lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology, Synonym: Endopeptidase K. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Sigma Aldrich Fine Chemicals Biosciences ISOBUTYRYL COENZYME A LITHIUM
NC3840085 ISOBUTYRYL COENZYME A LITHIUM
Sigma Aldrich Fine Chemicals Biosciences alpha Amylase from human s
amylase is the main secretory protein constituent of saliva. It contains 496 amino acids and has two isoforms glycosylated and non-glycosylated. amylase gene is located on human chromosome 1p21.1. amylase is mainly produced in pancreas and saliva.
Abcam Magenta-Gal, Chromogenic beta-galactosidase substrate, 500MG
MW 408.63 g/mol. Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product. Used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.
The product is subject to the following: Abcam Restricted Use Statement
ACROBiosystems Salt Active GENIUS™Nuclease, premium grade
Salt Active GENIUS™Nuclease, premium grade is expressed from Serratia marcescens E. coli.