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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
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Heclin is a small-molecule inhibitor of HECT-family E3 ubiquitin ligases used as a research reagent. It inhibits Smurf2, Nedd4, and WWP1 with micromolar potency and is used in studies of ubiquitin-mediated signaling and cancer biology. Supplied as a powder with recommended cold storage for stability.
Inhibits Smurf2, Nedd4, and WWP1 (IC50 ≈ 6-7 μM).
High purity (~98.2%) suitable for biochemical assays.
Available in small research quantities (5 mg) as a powder.
Recommended storage: powder -20°C for long term; in solvent -80°C for extended storage.
Intended for research use only; not for human or veterinary use.
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IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.
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Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.
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