Enzymes and Inhibitors
Cayman Chemical Coenzyme Q10 200mg
A cofactor in the electron transport chain; decreased levels due to genetic mutations in its synthesis enzymes lead to encephalopathy, cerebellar ataxia, infantile multisystemic form, nephropathy, and isolated myopathy
Bioworld HUMAN DNA TOPOISOMERASE-I (
HUMAN DNA TOPOISOMERASE-I (RECOMBINANT): Recombinant Human DNA Topoisomerase-I (Scl-70) Source:Sf9 insect cells. Background : Description : DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the requ...
Cayman Chemical ADHP Fluorescent
A highly sensitive, stable substrate for HRP that reacts with H2O2 to produce the fluorescent compound resorufin; enables detection of H2O2 at a concentration as low as 5 pmol per 100 µl samples
Sigma Aldrich Fine Chemicals Biosciences Hippuryl-His-Leu acetate salt | 103404-54-2 | 25MG
Hippuryl-His-Leu acetate salt | Purity: >=98% (HPLC) | Mol Wt: 429.47 | 103404-54-2 | 25MG
![Research Products International Corp Isopropyl-B-D-thio-galactopyranoside [IPTG], 1 Gram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-I56000-1.032.JPG-250.jpg)
Research Products International Corp Isopropyl-B-D-thio-galactopyranoside [IPTG], 1 Gram
IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.
Dioxane-Free
Water Content: <0.49%
New England Biolabs, Inc. APE 1 – 1000 units
Human apurinic/apyrimidinic (AP) endonuclease, APE 1, also known as HAP 1 or Ref-1, shares homology with Escherichia coli exonuclease III protein. APE 1 cleaves the phosphodiester backbone immediately 5 to an AP site, via hydrolytic mechanism, to generate a single-strand DNA break leaving a 3-hydroxyl and 5-deoxyribose phosphate terminus. Besides AP endonuclease activity, APE 1 has also been reported to have weak DNA 3 -diesterase, 3 to 5 exonuclease and RNase H activities (3-5). In addition to DNA repair activity, APE 1 is also capable of regulating the DNA binding activity of many transcription factors in vitro by a redox mechanism. As part of this process, APE 1 has been shown to stimulate the DNA binding activity of Fos-Jun heterodimers, Jun-Jun homodimers and Hela cell AP-1 proteins as well as that of several other transcription factors including NF-kB, Myb and members of the ATF/CREB family.
![Research Products International Corp X-a-Gal [5-Bromo-4-Chloro-3-indolyl-a-D-galactoside], 1 Gram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71900-1.014.JPG-250.jpg)
Research Products International Corp X-a-Gal [5-Bromo-4-Chloro-3-indolyl-a-D-galactoside], 1 Gram
Chromogenic substrate for detection of a-galactosidase activity in yeast Saccharomyces cerevisiae. Used in yeast Two-Hybrid System to detect protein-protein interactions within random libraries. The enzyme a-galactosidase enabels yeast to use disaccharide melibose as a carbon source during growth or fermentation. Secretion of this enzyme in response to activation of the GAL4 gene hydrolizes X-a-GAL and causes yeast colonies to develop a blue color. This reporter system allows immediate visible results on selection plates and eliminates necessity of a second screen filter life assay.
New England Biolabs, Inc. α2-3,6,8 Neuraminidase - 2000 units
2-3,6,8 Neuraminidase catalyzes the hydrolysis of 2-3, 2-6, and 2-8 linked sialic acid residues from glycoproteins and oligosaccharides.
New England Biolabs, Inc. Enterokinase, light chain – 2560 units
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
New England Biolabs, Inc. FokI – 5000 units
A restriction endonuclease that recognizes the sequence GGATGNNNNNNNNN^NNNN_.
New England Biolabs, Inc. HpyCH4III – 250 units
A restriction endonuclease that recognizes the sequence AC_N^GT.
New England Biolabs, Inc. Topoisomerase I (E. coli) – 100 units
Topoisomerase I (E. coli) catalyzes the relaxation of negatively supercoiled DNA. Topoisomerase I has also been implicated in knotting and unknotting DNA and in linking complementary rings of single-stranded DNA into double-stranded rings. The intact holoenzyme is a 97 kDa protein.
New England Biolabs, Inc. SnaBI – 2500 units
A restriction endonuclease that recognizes the sequence TAC_^GTA.