Modifying Enzymes
Apexbio Technology LLC EZ Cap OVA mRNA (5-moUTP) 5x1mg (1mg/mL)
Ovalbumin (OVA) mRNA encodes a egg white glycoprotein with a molecular weight of approximately 45 000 daltons It can induce moderate immunogenicity in organisms and is commonly used as an immunogen in immunological experiments such as establishing animal models for hypersensitivity asthma etc EZ Cap OVA mRNA (5-moUTP) is provided at 1 mg/mL capped by co-transcriptional capping with a Cap 1 structure and polyadenylated Modified 5-moUTP incorporation reduces the host cell immune response and enhances mRNA stability and translation efficiency
Sigma Aldrich Fine Chemicals Biosciences N Glycosidase f recombinan
Peptide-N-glycosidase f Peptide-N4-(acetyl-beta-glucosaminyl)-asparagine amidase
INTACT GENOMICS INC Exonuclease III 25000 Units (100 units/µl)
Exonuclease III (Exo III) is an exodeoxyribonuclease that digests one strand of duplex DNA from a blunt end, 5′ overhang or nick. The enzyme acts in a 3′→5′ direction producing stretches of single-stranded DNA (1, 2). Exo III is not active on 3′ overhangs of 4 or more bases in length and ssDNA. The enzyme has also RNase H, 3′-DNA phosphatase, and apurinic DNA endonuclease activities. Exo III digestions proceed at a uniform rate yielding predictable and reproducible results. However, the observed rate of nucleotide excision can vary depending on the individual characteristics of the reaction mix such as reaction temperature, ionic strength, template base content, template helical structure, and enzyme-to-DNA ratios.Applications• 3′ → 5′ exonuclease• Production of unidirectional nested deletions• Production of intermediates for site-directed mutagenesis• Preparation of strand-specific probes• Preparation of single-stranded substrates for dideoxy sequencing
ABclonal Technology T4 DNA Ligase (High Conc.)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. T4 DNA Ligase is applicable to cloning of restriction fragments and to joining linkers and adapters to blunt-ended DNA.
Athens Research And Technology Inc KALLIKREIN HU PLASMA 50UG
Kallikrein, Human Plasma: 50 UG; Frozen in 20 mM Tris-HCl, pH 7.8, with 100 mM NaCl; MW 86,000-88,000; EC= 1.06; Activity: > =15 U/MG; Purity: > =95% by SDS-PAGE. Native; Prepared and purified from human plasma. Mol. Biology Reagent, Serine Protease
Sino Biological Recombinant DNase I (RNase-free) 5000 U
DNase I is an endonuclease that can non-specifically cleave DNA, producing dinucleotide, trinucleotide, and oligonucleotide products with 5 ́ phosphorylated and 3 ́ hydroxylated ends. DNase I acts on single-and double-stranded DNA, chromatin, and RNA:DNA hybrid strands. Certified by highly sensitive fluorescence probe assays, our DNase I (RNase-free) demonstrates virtually undetectable RNase activity-a level of purity that surpasses other commercial sources. Trust in this "Ultra-Pure" standard for the ultimate reliability in your RNase-sensitive workflows.
Cayman Chemical N-Oleoyl ValIn 25mg
An endogenous N-acyl amine; antagonist at TRPV3; increases after acute lung injury in mouse lung tissue
ABclonal Technology Endonuclease IV
Endonuclease IV derived from E. coli recognizes the depurine/depyrimidine (AP) site on the double- stranded DNA molecule, and cleaves the first phosphodiester bond at the 5 ?end of the AP site to form 3-OH and 5dRP . In addition, Endonuclease IV has 3?diesterase activity, which releases phosphoglyceraldehyde, intact deoxyribose 5 -phosphate, and phosphoric acid from the 3 ?end of DNA. The best substrate for this enzyme is AP double-stranded DNA, but it is also active against AP single-stranded DNA.
Biotium CD45 / LCA (Leucocyte Marker)(Bra55), CF488A conjugate, 0.1mg/mL
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Apexbio Technology LLC PreScission Protease (PSP), 100U (1U/uL). Cas: MFCD: NA. Specifically recognize and cleave the Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro peptide in fusion proteins.
PreScission Protease is a fusion protein of human rhinovirus 3C protease and glutothione S trasferase (GST). PreScisson protease can specifically recognize the Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro sequence and cleaves between the Gln and Gly residues. Concentration: 2U/L Other sizes are also available. Please inqury us for quote.
ABclonal Technology RNase A (Powder)
Ribonuclease A (RNase A), molecular weight 13.7 kDa, is an endonuclease that specifically acts on the 3 end of the pyrimidine residue on RNA, cleaving phosphodiester bonds formed with adjacent nucleotides, and the final product of the reaction is pyrimidine 3 phosphate and an oligonucleotide with pyrimidine 3 phosphate at the end. RNase A does not work on DNA.This product is sourced from bovine pancreas and is supplied in the form of white lyophilized powder with enzyme activity ≥ 50 Kunitz units/mg protein. Its recommended working concentration is 1-100 μg/mL, which will vary depending on specific applications.
ABclonal Technology T7 DNA Ligase
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It can catalyze the formation of a phosphodiester bond between adjacent 5 phosphate and 3 hydroxyl termini in double-stranded DNA. T7 DNA ligase can catalyze ligation of cohesive ends and repair nicks, but not blunt end ligation. This makes a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined .