Modifying Enzymes
New England Biolabs, Inc. T4 DNA Ligase – 20,000 units – 2,000,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
New England Biolabs, Inc. Lambda Exonuclease – 1000 units
Lambda Exonuclease is a processive enzyme that catalyzes 5' to 3' mononucleotide removal from dsDNA with a preferred substrate of a 5' phosphate. Ideal for conversion of linear double-stranded DNA to single-stranded DNA via preferred activity on 5'-phosphorylated ends.
Worthington Biochemical Corporation CATALASE 10ML
Catalase, Filtered, Minimum Activity: 40000U/mg Protein, Supplied as an aqueous solution of 2X crystallized catalase filtered through a 0.22u pore size Membrane, Store at 2 deg. to 8 deg.C, 10mL
INTACT GENOMICS INC Taq DNA Ligase, 2,000 Units (50 µl)
Taq DNA Ligase provides high specificity for ligation-based PCR, SNP detection, and molecular diagnostics. This enzyme catalyzes phosphodiester bond formation in duplex DNA, using NAD+. It enables precise, high-temperature ligation (45°C to 70°C), suitable for applications requiring accuracy, including single-base substitution detection.
- High specificity for PCR, SNP detection, and molecular diagnostics.
- Enables precise, high-temperature DNA ligation (45°C to 70°C).
- Catalyzes phosphodiester bond formation in duplex DNA with NAD+ cofactor.
- Detects single-base substitutions; protein purity ≥99%.
- Free from detectable RNase and DNA endonuclease activities.
- Ideal for allele-specific gene detection (LDR, LCR) and mutagenesis.
- Includes Taq DNA Ligase and 10X Taq DNA Ligase Buffer.
Bioworld Alkaline Phosphatase in 50% Glycerol, 5000 Unit(s)
Alkaline Phosphatase in 50% GlycerolSpecifications:Source: Calf Intestine.Supplied in 50% GlycerolActivity: 3000 - 4000 U/mg proteinApplication: as an indicator enzyme when conjugted to proteins such as antibodies, avidin etc. Suitable also for western blot analysis.Unit definition: the amount of enzyme catalyzing the liberation of 1 μmole of p-nitrophenyl phosphate per minute at 37° C, pH 9.8 in diethanolamine buffer, 405nm.If assayed at 25°C in glycine buffer, pH 9.6, then, 1 glycine unit= 3DEA units
Bioworld Beta-Glactosidase, 5000 Unit(s)
Beta-Galactosidase Enzyme found in lac+ bacterial cells that catalyzes the cleavage of -linked terminal galactose-containing substratesLyophilized powderSource: E. coliConjugation gradeActivity (37°C):800-1000U/mg proteinUnit definition: One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH7.3 at 37°C, 410nm.Aggregation (HPLC): <3.0%Application: Used as an indicator enzyme conjugated to proteins such as antibodies.
Worthington Biochemical Corporation Ribonuclease A, Recombinant, Protease & DNase Free, AF, 10 ku, Code- RRA1
Ribonuclease A, Recombinant, Protease & DNase Free, AF, 10 ku. Source: Komagataella phaffii (previously P. pastoris)Recombinant bovine pancreatic Ribonuclease A produced in Komagataella phaffii. Code- RRA1
Cayman Chemical Decnoyl-Coenzyme Ahydrate 1mg
A thioester of decanoic acid and CoA; inhibits the activity of mitochondrial CS and GDH isolated from rat brain (IC50s = 437 and 420 µM, respectively); inhibits FadR from binding its promoter in E. coli (Ki = 2 µM); produced during isomerase-dependent β-oxidation of oleic acid in isolated rat heart mitochondria
Worthington Biochemical Corporation DNASE I DPFE 5MG
Deoxyribonuclease I, Chromatographically purified, A lyophilized powder containing glycine as a stabilizer, Contains /=2000 Kunitz U/mg dry
Medchemexpress LLC Methanone, 2-benzofuranyl(4-nitrophenyl)- | 93944-58-2 | 99.49% | 267.24 | 5 MG
α-Amylase-IN-3 (Compound 4) is an inhibitor for α-Amylase, AChE, and BChE, with IC50s of 18.04 μM, 21.04 μM, and 22.2 μM, respectively. It exhibits antioxidant activity and can be used in research related to diabetes and oxidative stress-associated diseases. This product is provided as a white to off-white solid.
- Inhibitor for α-Amylase, AChE, and BChE
- Exhibits antioxidant activity
- Used in research for diabetes
- Used in research for oxidative stress-associated diseases
- White to off-white solid appearance
Bioss KD-Validated Topoisomerase II
DNA Topoisomerase II Alpha Primary Antibody Unconjugated Public Immunogen Range 362-400/400 Host Species Rabbit Target Species Human Mouse Rat Clonality Monoclonal Applications WB FCM IC Concentration Lot dependent Size 50 ul Storage Buffer Supplied in PBS (pH 7 4) containing 50% glycerol and 0 02% sodium azide
Pfaltz & Bauer Glucose oxidase with catalase from Aspergillus Niger 100 KU/gram minimum 1G
Glucose oxidase with catalase from Aspergillus Niger 100 KU/gram minimum 1G
Medchemexpress LLC Bacterial α-Amylase | 9000-85-5 | ≥5000 U/g solid | 10 G
Bacterial α-Amylase from MedChemExpress is an enzyme that catalyzes the hydrolysis of internal α-1,4-glycosidic linkages in starch, breaking it down into smaller units like glucose, maltose, and maltotriose. It is commonly employed in various biochemical studies.
- Hydrolyzes α-1,4-glycosidic linkages in starch
- Produces low molecular weight products (glucose, maltose, maltotriose)
- Suitable for biochemical studies
- Wide temperature-activity optima (25°C to approximately 100°C)
- Broad pH optima (1 to 11.5)
ABclonal Technology T4 RNA Ligase 1
T4 RNA ligase 1 catalyzes the formation of a 3 to 5 phosphodiester bond by joining the 5 phosphate end of a nucleotide to the 4 hydroxyl end, accompanied by the hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA, DNA, and dinucleoside pyrophosphates.
Cayman Chemical Oleoyl-L-carnItInchlorIde 25mg
A long-chain acylcarnitine and GlyT2 inhibitor (IC50 = 340 nM); selective for GlyT2 over GlyT1 (IC50 = >10,000 nM); plasma levels are increased in patients with chronic kidney disease or end-stage renal disease receiving incident hemodialysis and these are associated with cardiovascular mortality