Modifying Enzymes
New England Biolabs, Inc. T4 Polynucleotide Kinase – 500 units
T4 PNK catalyzes the transfer and exchange of P from the gamma; position of ATP to the 5 -hydroxyl terminus of polynucleotides (double-and single-stranded DNA and RNA) and nucleoside 3-monophosphates. Polynucleotide Kinase also catalyzes the removal of 3-phosphoryl groups from 3-phosphoryl polynucleotides, deoxynucleoside 3-monophosphates and deoxynucleoside 3-diphosphates.
- 5' phosphorylation of DNA/RNA for subsequent ligation
- End labeling DNA or RNA for probes and DNA sequencing
- Removal of 3' phosphoryl groups
New England Biolabs, Inc. T4 RNA Ligase 1 (ssRNA Ligase) – 5000 units
T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3' - 5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
Worthington Biochemical Corporation HYALURONIDASE PURIFIED 5KUHYA
Chromatographically purified. A dialyzed, lyophilized powder.
New England Biolabs, Inc. Exonuclease I (E.coli) – 3000 units
Exonuclease I is a DNA-specific endonuclease that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction. Note: See also Exo-CIP Rapid PCR Cleanup Kit.
New England Biolabs, Inc. T4 DNA Ligase – 100,000 units – 400,000 units/ml
The industry standard for performance and quality. T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA.
- Catalyzes the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA
- Joins blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA
- T4 DNA Ligase variants: Salt-T4, Hi-T4, Instant Sticky-end Ligase MM, Blunt/TA Ligase MM
New England Biolabs, Inc. T4 RNA Ligase 1 (ssRNA Ligase) – 1000 units
T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3' - 5' phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
New England Biolabs, Inc. T7 RNA Polymerase – 25000 units
Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters. The 99 KD enzyme catalyzes in vitro RNA synthesis from a cloned DNA sequence under the T7 promoters. RNA produced using the T7 RNA Polymerase is suitable for many applications in research and biotechnology. Reaction Conditions 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and DNA template containing the T7 RNA Polymerase promoter. Incubate at 37°C.
New England Biolabs, Inc. T4 RNA Ligase 2, truncated – 10000 units
T4 RNA Ligase 2' truncated (T4 Rnl2'truncated) specifically ligates the pre-adenylated 5' end of DNA or RNA to the 3 end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. T4 Rnl2'truncated is expressed from a plasmid in E. coli which encodes the first 2'9 amino acids of the full length T4 RNA Ligase 2' Unlike the full length ligase, T4 Rnl2'truncated is unable to adenylate the 5' end of the substrate, and as a result it cannot ligate the phosphorylated 5' end of RNA or DNA to the 3' end of RNA . This enzyme, also known as Rnl2'(1-2'9) has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers).
Sigma Aldrich Fine Chemicals Biosciences Benzonase(R) endonuclease EMPROVE(R) Expert | 9025-65-4 | MFCD00131010 |
Benzonase(R) endonuclease EMPROVE(R) Expert | 9025-65-4 | MFCD00131010 |
Sigma Aldrich Fine Chemicals Biosciences Phosphatase, Alkaline-Agarose from calf intestine ammonium sulfate suspension | MFCD00131852 | 100UN
Phosphatase, Alkaline-Agarose from calf intestine ammonium sulfate suspension | MFCD00131852 | 100UN
New England Biolabs, Inc. BspQI – 2500 units
A restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_.
Sigma Aldrich Fine Chemicals Biosciences Pectinase from Aspergillus aculeatus | 50 mL
Pectinase is an enzyme from Aspergillus aculeatus that is used in plant protoplast preparation to digest cell wall prior to organelle isolation. It has been used to conduct partial saccharification of sugars. Pectinases are used to study their role in the invasion of plant tissues by phytopathogens, the spoilage of produce and various food processing and plant biotechnology applications. Pectinase, product P2611, has been used to treat leaf discs from fifth wild-type and sitiens leaves.
Sigma Aldrich Fine Chemicals Biosciences Hyaluronidase from bovine testes | 37326-33-3 | MFCD00131351 | 5 g
Hyaluronidase from bovine testes | Mol Wt: ~55 kDa | 37326-33-3 | MFCD00131351 | 5 g
Zymo Research Corporation RNASE A, 30 mg
RNase A specifically cleaves at the 3’-side of pyrimidine (uracil or cytosine) phosphate bonds. The enzyme does not hydrolyze DNA, because DNA lacks 2’-OH groups essential for the formation of cyclic intermediates. The enzyme can also be used to hydrolyze RNA from protein samples. It is compatible for use in RNase protection assays, to remove unspecifically bound RNA,in the analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and in the purification of DNA.