Modifying Enzymes
New England Biolabs, Inc. HiFi Taq DNA Ligase – 50 reactions
An optimized blend of a thermostable DNA Ligase and a proprietary additive, HiFi Taq DNA Ligase efficiently seals nicks in DNA with unmatched high fidelity. The formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini of two adjacent oligonucleotides that are hybridized to a complementary target DNA is enhanced in the improved reaction buffer and mismatch ligation is dramatically reduced. The improved formulation allows higher resolution discrimination between ligation donors and acceptors, enabling precise detection of SNPs and other allele variants. HiFi Taq DNA Ligase is active at elevated temperatures (37-75C). Please note that HiFi Taq DNA Ligase is intended for use in molecular diagnostics applications that depend on high fidelity nick ligation. It is not a substitute for T4 DNA ligase and is not suitable for cloning applications or adapter ligation/NGS library prep.
Sigma Aldrich Fine Chemicals Biosciences Phosphoglucose Isomerase from baker's yeast (S. cerevisiae) Type III, ammonium sulfate suspension, >=400 units/mg protein (biuret) | 9001-41-6 | MFCD00131887 | 1KU
Phosphoglucose Isomerase from baker's yeast (S. cerevisiae) Type III, ammonium sulfate suspension, >=400 units/mg protein (biuret) | 9001-41-6 | MFCD00131887 | 1KU
Sigma Aldrich Fine Chemicals Biosciences Chitinase from Trichoderma viride lyophilized powder, >=600 units/g solid | MFCD00130771 | 25UN
Chitinase from Trichoderma viride lyophilized powder, >=600 units/g solid | MFCD00130771 | 25UN
Abcam Proteinase K Antigen Retrieval Solution, 4ML
Proteinase K antigen retrieval solution is a ready-to-use, single-component solution designed for proteolytic-induced antigen retrieval (PIER) in immunohistochemistry (IHC) and pre-treatment for in situ hybridization (ISH). - Cited in over 25 publications - Ready-to-Use – no preparation needed for immediate application - Incubate tissue section for 5-7 minutes at room temperature in IHC - Incubate tissue section for 15 minutes at 37°C in ISH
The product is subject to the following: Abcam Restricted Use Statement
LGC GENOMICS LLC (Lucigen) CircLigase ssDNA Ligase, 5,000 U @ 100 U/LA thermostable ligase that catalyses the intramolecular ligation (i.e. circularisation) of ssDNA and ssRNA templates.
CircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5-phosphate and a 3-hydroxyl group
New England Biolabs, Inc. Thermolabile Exonuclease I – 15000 units
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3' to 5' direction.
LGC GENOMICS LLC (Lucigen) Riboguard RNase Inhibitor, 2500 units @ 40 U/LRNA is precious. Protect it with this high quality, effective RNase inhibitor. Manufactured under ISO-13485.
RiboGuard™ RNase Inhibitor is a recombinant protein that is the best defense against common RNases, including RNase A, RNase B, and RNase C.
Sigma Aldrich Fine Chemicals Biosciences Protease from Aspergillus oryzae | 1395-21-7 | MFCD00132092 | 250mL
Protease from Aspergillus oryzae | 1395-21-7 | MFCD00132092 | 250mL
LGC GENOMICS LLC (Lucigen) Terminator 5'-Phosphate-Dependent Exonuclease, 40U @ 1 U/LA processive 5 to 3 riboexonuclease that specifically digests RNA with 5-monophosphate ends only.
Terminator™ 5-Phosphate-Dependent Exonuclease is a processive 53 exonuclease that digests RNA that has a 5-monophosphate end. It does not digest RNA that has a 5-triphosphate, 5-cap or 5-hydroxyl group.
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium album, 39450-01-6, MFCD00132129, 25 mg
Lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology, Synonym: Endopeptidase K. Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Sigma Aldrich Fine Chemicals Biosciences ISOBUTYRYL COENZYME A LITHIUM
NC3840085 ISOBUTYRYL COENZYME A LITHIUM
New England Biolabs, Inc. XRN-1 – 20 units
XRN-1 is highly processive 53 exoribonuclease, requiring 5 monophosphate. It also acts on 5' monophosphate ssDNA with greatly reduced efficiency.
Ec-Oxyrase Enzyme System, Sterile, 50Ml Bottle 1/Pk
Oxyrase Unit defined as amount of activity that reduces dissolved oxygen @ 37°C & pH 8.4 in a phosphate buffer with lactate substrate @ rate of 1% per second. 0.3 unit of EC-Oxyrase reduces dissolved oxygen @ saturation to levels measured in parts per billion (ppb) in about 4 minutes. Active over wide temperature range. Operates over wide pH range 6.8 to 9.4.
Sigma Aldrich Fine Chemicals Biosciences Beta-Glucuronidase from Helix pomatia | 9001-45-0 | MFCD00130629 | 1 million units
Beta-Glucuronidase from Helix pomatia | 9001-45-0 | MFCD00130629 | 1 million units