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Sigma Aldrich Amano Lipase From Pseudomonas Fluorescens
MilliporeSigma Sigma Organics products encompass a wide range of quality reagents, solvents, catalysts, and building blocks for organic synthesis. From benchtop discovery to process development and scale-up, Sigma Organics solutions are built to meet the needs of synthetic chemists.
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| Boiling Point | specific activity: ≥20,000 U/g, pH 8.0, 55 C (Optimum pH and temperature); specific activity: ≥20,000 U/g |
|---|---|
| CAS | 9001-62-1 |
| MDL Number | MFCD02685888 |
| Health Hazard 1 | Non-Hazardous |
| RTECS Number | TO9776500 |
| Recommended Storage | Room Temperature |
| EINECS Number | 232-619-9 |
Promega SP6 RNA Polymerase
DNA-dependent RNA polymerase for in vitro transcription.
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Promega RQ1 RNase-Free DNase
RQ1 (RNA Qualified) RNase-Free DNase is a DNase I that degrades both double-stranded and single-stranded DNA.
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Promega Factor Xa Protease
Factor Xa Protease preferentially cleaves after the arginine residue in the amino acid sequence Ile-Glu-Gly-Arg.
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Promega Terminal Deoxynucleotidyl Transferase
TdT catalyzes the addition of mononucleotides to the terminal 3'-OH of a DNA initiator accompanied by the release of inorganic phosphate.
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| Components | 5X Reaction Buffer: 500mM Cacodylate Buffer (pH 6.8 at 25°C), 5mM CoCl2, 0.5mM DTT |
|---|---|
| Content And Storage | -30°C to -10°C |
| Form | Liquid |
| Product Type | Terminal Deoxynucleotidyl Transferase |
| Storage Buffer | 50mM Potassium Phosphate (pH 6.4), 100mM NaCl, 1mM beta-mercaptoethanol, 0.1% Tween™ 20, 50% Glycerol |
| Color | Colorless |
| pH | 6.4 |
| Concentration | 30 U/μL |
| For Use With (Application) | Tailing reactions to add complementary Homopolymer tails to DNA vectors and cDNA, 3' end-labeling, TUNEL Assays |
| Source | Recombinant E. coli strain |
Promega 4-CORE™ Buffer Pack
Consists of one vial each of Restriction Enzyme Buffers A, B, C and D.
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Promega Proteinase K
Serine protease that exhibits a very broad cleavage specificity. Useful in many molecular biology applications.
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Thermo Scientific™ Exonuclease VII
A strict single-strand directed enzyme with 5'→3' and 3'→5' exonuclease activities.
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Promega Modifying Enzymes: Single-Stranded DNA Binding Protein
Binds with high affinity in a cooperative manner to single-stranded DNA but does not bind well to double-stranded DNA
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Promega T3 RNA Polymerase
DNA-dependent RNA polymerase for in vitro transcription.
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| Components | 100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl |
|---|---|
| Content And Storage | -30°C to -10°C |
| Form | Liquid |
| Product Type | T3 RNA Polymerase |
| Storage Buffer | 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol |
| Color | Colorless |
| pH | 7.7 |
| Purity | >90% |
| For Use With (Application) | Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA |
| Source | Recombinant E. coli strain |
Promega S1 Nuclease
Endonuclease specific for single-stranded RNA or DNA.
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Promega Ribonuclease H
RNase H is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA.
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| Content And Storage | -30°C to -10°C |
|---|---|
| Form | Liquid |
| Product Type | Ribonuclease H |
| Storage Buffer | 20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 0.2mg/mL BSA, 50% Glycerol |
| Color | Colorless |
| pH | 7.8 |
| Concentration | 0.5 to 2 U/μL |
| For Use With (Application) | Removal of the RNA strand prior to second strand cDNA synthesis, analysis of in-vitro polyadenylation reaction products, microarrays |
| Source | Recombinant E. coli strain |
Promega T7 RNA Polymerase
DNA-dependent RNA polymerase for in vitro transcription.
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| Components | 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl |
|---|---|
| Content And Storage | -30°C to -10°C |
| Inhibitors | Recombinant RNasin™ Ribonuclease |
| Form | Liquid |
| Product Type | T7 RNA Polymerase |
| Storage Buffer | 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol |
| Color | Colorless |
| pH | 7.7 |
| Purity | >90% |
| For Use With (Application) | Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA |
| Source | Recombinant E. coli strain |
Promega Streptavidin Alkaline Phosphatase
Used for the detection of biotinylated molecules.
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Promega T4 DNA Ligase Buffer Pack
For ligation of DNA ends.
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