Modifying Enzymes
G-Biosciences T4 UvsX DNA Recombinase
T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes.
End-Repair dA-tailing Mix, 24 reactions, G-Biosciences
The End-Repair dA-tailing Mix converts damaged or incompatible 5'/3′ ends to 3' A tailed and 5′-phosphorylated DNA.
T4 RNA ligase I, 5000 U, G-Biosciences
T4 RNA Ligase I (gp63) catalyzes the ATP-dependent ligation of single-stranded nucleic acids by joining a 5′ phosphate to a 3′ hydroxyl. It is most efficient at ligating the 3′-OH of RNA to the 5′-phosphate of RNA or DNA in single-stranded contexts.
T7 DNA Ligase, 75000 U, G-Biosciences
T7 DNA Ligase is an ATP-dependent enzyme from bacteriophage T7 that catalyzes phosphodiester bond formation between 5′ phosphate and 3′ hydroxyl termini of duplex DNA.
T7 Exonuclease, 2500 U, G-Biosciences
Gene 6 protein of bacteriophage T7 is a dsDNA 5' to 3' exonuclease which also exhibits flap endonuclease activity.
New England Biolabs, Inc. EcoRI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
FUJIFILM BIOSCIENCES INC Lysyl Endopeptidase, Mass Spectrometry Grade (Lys-C), 5 x20UG
*Orders for this supplier are non-cancellable/non-returnable once they are processed.* Lysyl Endopeptidase, Mass Spectrometry Grade. For Proteome Research. 20 ug x 5 vials.
Innovative Cell Technologies ACCUTASE CELL DETACHMENT REAGN
Accutase; Sterile; 100mL Enzyme cell detachment medium; Composition: Proprietary formulation of enzymes, water, phosphate buffered saline, phenol red; Stability: Stable under normal temperatures and pressures
New England Biolabs, Inc. Shrimp Alkaline Phosphatase (rSAP) – 500 units
Shrimp Alkaline Phosphatase (rSAP) is a heat labile alkaline phosphatase purified from a recombinant source. rSAP is identical to the native enzyme and contains no affinity tags or other modifications. rSAP nonspecifically catalyzes the dephosphorylation of 5 and 3 ends of DNA and RNA phosphomonoesters. Also, rSAP hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). rSAP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5 protruding, 5 recessed and blunt ends. rSAP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. rSAP is completely and irreversibly inactivated by heating at 65C for 5 minutes, thereby making removal of rSAP prior to ligation or end-labeling unnecessary.
LGC GENOMICS LLC (Lucigen) Plasmid-Safe ATP-Dependent DNase, 1000 U @ 10 U/LDigest unwanted contaminating linear double-stranded DNA, without harming circular double-stranded DNA such as plasmids and fosmids.
Plasmid-Safe™ ATP-Dependent DNase selectively removes contaminating bacterial chromosomal DNA from plasmid, cosmid, fosmid, and BAC clones or vector preparations.
Worthington Biochemical Corporation DNASE 1 100MG
Deoxyribonuclease I, 100 MG, Purified precrystalline DNase. A lyophilized powder. Store at 2-8degreeC., Source: Bovine Pancreas, Minimum Activity: =2,000 Kunitz units per mg dry weight
New England Biolabs, Inc. Rapid™ PNGase F – 50 reactions
Rapid PNGase F enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.
- Complete deglycosylation of antibodies and immunoglobulin fusion proteins in 10 minutes
- Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
- All reaction components are compatible with HPLC and mass spectrometry analysis
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability ensured for up to 12 months
LGC GENOMICS LLC (Lucigen) Proteinase K, 2 mLDigest proteins during DNA and RNA purification or inactivate enzymes. To be used with the MasterPure Kits.
This reagent is designed for use with the MasterPure™ kit protocols only.
Sigma Aldrich Fine Chemicals Biosciences DNASE I GRADE II, FROM BOVINE
DNase I grade II, from bovine pancreas 100mg