Modifying Enzymes
T7 Exonuclease, 2500 U, G-Biosciences
Gene 6 protein of bacteriophage T7 is a dsDNA 5' to 3' exonuclease which also exhibits flap endonuclease activity.
End-Repair dA-tailing Mix, 24 reactions, G-Biosciences
The End-Repair dA-tailing Mix converts damaged or incompatible 5'/3′ ends to 3' A tailed and 5′-phosphorylated DNA.
T7 DNA Ligase, 75000 U, G-Biosciences
T7 DNA Ligase is an ATP-dependent enzyme from bacteriophage T7 that catalyzes phosphodiester bond formation between 5′ phosphate and 3′ hydroxyl termini of duplex DNA.
T4 RNA ligase I, 5000 U, G-Biosciences
T4 RNA Ligase I (gp63) catalyzes the ATP-dependent ligation of single-stranded nucleic acids by joining a 5′ phosphate to a 3′ hydroxyl. It is most efficient at ligating the 3′-OH of RNA to the 5′-phosphate of RNA or DNA in single-stranded contexts.
G-Biosciences T4 UvsX DNA Recombinase
T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes.
LGC GENOMICS LLC (Lucigen) Ampligase DNA Ligase with Buffer, 750 U @ 5 U/LHighly thermostable DNA ligase for applications where ligation at high temperature is beneficial or required.
Ampligase Thermostable DNA Ligase catalyzes NAD-dependent ligation of adjacent 3-hydroxylated and 5-phosphorylated termini in duplex DNA structures that are stable at high temperatures
New England Biolabs, Inc. EcoRI-HF – 10000 units
A high fidelity restriction endonuclease that recognizes the sequence G^AATT_C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Proteinase K, Molecular Biology Grade – 2 x 1 ml vials
Proteinase K is a subtilisin-related serine protease that hydrolyzes a variety of peptide bonds and is frequently used to cleanup enzymatic reactions or cell lysates.
Innovative Cell Technologies ACCUTASE CELL DETACHMENT REAGN
Accutase; Sterile; 100mL Enzyme cell detachment medium; Composition: Proprietary formulation of enzymes, water, phosphate buffered saline, phenol red; Stability: Stable under normal temperatures and pressures
Sigma Aldrich Fine Chemicals Biosciences DNASE I GRADE II, FROM BOVINE
DNase I grade II, from bovine pancreas 100mg
LGC GENOMICS LLC (Lucigen) CircLigase II ssDNA Ligase, 5,000 U @ 100 U/LAn ATP-independent, non-catalytic thermostable ligase that catalyses the intramolecular ligation (i.e. circularisation) of ssDNA and ssRNA templates.
CircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5-phosphate and a 3-hydroxyl group
LGC GENOMICS LLC (Lucigen) Proteinase K, 2 mLDigest proteins during DNA and RNA purification or inactivate enzymes. To be used with the MasterPure Kits.
This reagent is designed for use with the MasterPure™ kit protocols only.
Worthington Biochemical Corporation DNASE 5 VIAL/BOX
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).