Bluo-Gal is a chromogenic substrate used to determine the presence or absence of a cloned DNA inserted in bacteria growing on agar plates. When hydrolyzed by beta-galactosidase, the resulting product will form a blue precipitate. The precipitate is insoluble in alcohol and xylenes, thus it is suitable for immunoblotting and immunocytochemical assays. Upon microscopic examination under polarized light, the precipitated crystals emit a strong signal consisting of yellow reflected light. Bluo-Gal can be used as an alternative of X-Gal. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.CAS Number: 97753-82-7Molecular Weight: 374.2 DaChemical Formula: C14H16BrNO6Solubility: Clear and colorless solution (Sol. 2%, DMF)Storage Temperature: -20CResearch or further manufacturing use only, not for food or drug use.