DNA Extraction and Purification
Zymo Research Corporation Femto™ Bacterial DNA Quantification Kit
Bacterial DNA can be reliably quantified in a background of non-bacterial DNA such as fungal, animal, and plant DNA, etc. This is essential for downstream applications that require accurate DNA input including quantifying bacteria DNA template for Next-generation sequencing library preparation and metagenomic analysis amongst others. With the Femto™ BacterialDNA Quantification Kit, one can dependably quantify as little as 20 fg DNA from 1 l purified from biological liquids, bacterial cultures, or environmental DNA samples.
New England Biolabs, Inc. NEBNext dsDNA Fragmentase – 250 reactions
NEBNext dsDNA Fragmentase is an enzyme-based reagent that shears DNA to produce fragments of the desired sizes in a time-dependent manner, for next generation sequencing library preparation protocols Note: NEBNext dsDNA Fragmentase is not part of the NEBNext Ultra II FS (Fragmentation System) reagents.
Omega Biotek Inc Ultra-Pure Plasmid DNA 96 Kit, 4 x 96 preps
Ultra-Pure Plasmid DNA 96 Kit combines the power of Mag-Bind technology with the innovative ETR technology (Endo-toxin removal) to deliver high-quality endotoxin-free plasmid DNA in high throughput format.
Norgen Biotek Corp Bacterial Genomic DNA Isolation Kit
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, Sequencing and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Norgen Biotek Corp CleanAll DNA/RNA Clean-Up and Concentration Micro Kit
Norgen's CleanAll DNA/RNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications. The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. The kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Endotoxins can be removed from previously purified RNA solutions down to 0.1 EU/µg DNA or less. The kit can be used to clean up DNA from digestions, ligations, PCR reactions, DNA modification reactions and staining.Purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA.Purify all sizes of DNA, from small PCR products, native or linearized plasmids, oligonucleotides, to genomic DNA.
New England Biolabs, Inc. Monarch® Spin Plasmid Miniprep Kit - 250 preps
The Monarch Spin Plasmid Miniprep kit, a newer version of our original Monarch Miniprep kit, offers a unique method for plasmid preparation and purification, enabling extraction of highly concentrated plasmid DNA from various bacterial strains, with yields of up to 20 ug of high-quality DNA. This kit features upgraded spin columns precision-engineered to uniquely allow for low elution, in as little as 30 ul, for highly concentrated DNA in downstream application such as sequencing, restriction digestion, transformation, transfection, and more. A colored buffer system helps indicate completion of key lysis and neutralization steps for an easy to follow workflow and shorter, faster protocols allow for reduced hands-on time and less spin time. Compared to leading suppliers, these kits use significantly less plastic through uniquely designed columns and bottles with plastic reduction while still maintaining structural integrity for centrifugation or vacuum manifolds and other steps
Epigentek Inc Epigenase Universal SIRT Activity/Inhibition Assay Kit (Colorimetric) (96 assays)
The Epigenase Universal SIRT Activity/Inhibition Assay Kit (Colorimetric) is a complete set of optimized buffers and reagents for measuring the activity/inhibition of total SIRT enzymes using nuclear extracts or purified SIRT isoforms (SIRTs 1-7) from a broad range of species such as mammals, plants, fungi, and bacteria, in a variety of forms including, but not limited to cultured cells and fresh and frozen tissues. Detection of inhibition or activation of SIRTs is important in elucidating mechanisms of epigenetic regulation of gene activation and silencing and may benefit diagnostics and therapeutics of cancer or neurological diseases.
Epigentek Inc Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Colorimetric) (96 assays)
For measuring enzymatic activity or inhibition levels of TET
Chemgenes Corporation Medium Drying Trap, 10 PK, suitable for oligonucleotide synthesis, 50-100 mL acetonitrile solution, Reduces moisture <50 ppm, Fully activated
Medium Drying Trap, 10 PK, suitable for oligonucleotide synthesis, 50-100 mL acetonitrile solution, Reduces moisture <50 ppm, Fully activated
Plasmid MegaPrep kit, 16 samples, yield up to 5 mg, endotoxin level <0.1 EU/μg, supercoiled DNA, elution volume 18 mL, A260/A280 purity >1.8, A260/A230 purity >2.0, compatible with Biotage PhyPrep system
Plasmid MegaPrep kit, 16 samples, yield up to 5 mg, endotoxin level <0.1 EU/μg, supercoiled DNA, elution volume 18 mL, A260/A280 purity >1.8, A260/A230 purity >2.0, compatible with Biotage PhyPrep system
Research Products International Corp DNA Binding Buffer, 100 Milliliter
DNA Binding Buffer is designed for use in the DNA Clean & Concentrator family of products. This buffer facilities efficient binding and recovery of DNA from enzymatic reactions and impure DNA samples.
Biochain Institute Inc Genomic DNA - Human Tumor Tissue: Colon Tumor, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Spleen, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.