DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Human Tumor Cell Line: Hela, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Phenomenex Inc 4g MgSO4, 1g NaCL, 1g Na3Cit2H2O, 0.5g Na2Cit1.5
MAS-Q Extraction Packets MS-NMS5050-1-S
Zymo Research Corporation DNA/RNA Shield Collection Tube (50 pack)
DNA/RNA Shield Lysis and Collection tubes are designed for the streamlined collection, transport, and processing of biological specimen. They are pre-filled with DNA/RNA Shield and a lysis matrix tailored for tough-to-lyse- sample inputs from tissue to microbial samples. Collected samples do not require additional handling for processing and are ready for homogenization.
Active Motif UDP-Azide-Glucose
UDP-Azide-Glucose is used in conjunction with Beta-glucosyltransferase enzyme (Catalog No. 55012) to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation for analysis. For more complete information, please visit the Active Motif website.
Norgen Biotek Corp FFPE RNA/DNA PURIFICATION KIT
Norgen’s FFPE RNA/DNA Purification Plus Kit provides a rapid method for the sequential isolation and purification of total RNA (including microRNA) and genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. RNA and DNA are sequentially purified into separate fractions using this kit. The kit provides conditions that allow for the partial reversing of the formalin modifications, resulting in high quality and yield of RNA/DNA. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as the degree of fragmentation of the RNA will increase over time. RNA is purified without using phenol or chloroform. Purified RNA is of the highest integrity and can be used in a number of downstream applications including real time PCR. Purified genomic DNA is also of the highest quality, and can be used in sequencing.
Norgen Biotek Corp Fungi/Yeast Genomic DNA Isolation Kit
Norgen's Fungi/Yeast Genomic DNA Isolation kit provides rapid spin column purification of genomic DNA from viable yeast cells, fungal spores or mycelium, and Gram-positive and Gram-negative bacteria. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and bead tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. Protocol time is typically less than 30 minutes. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.
Research Products International Corp Genomic DNA Clean & Concentrator Kit-25, 100 Preps
The Genomic DNA Clean & Concentrator-25 (DCC) is for quick (5 minute) recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Bufferto a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. The product is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, (wga)DNA, etc.) from anyenzymatic reaction or impure preparation (e.g., Proteinase K digestion). No messy precipitations!
LGC GENOMICS LLC (Lucigen) QuickExtract Plant DNA Extraction SolutionRapid and efficient extraction of PCR-ready genomic DNA from plant leaf samples.
QuickExtract Plant DNA Extraction SolutionRapid and efficient extraction of PCR-ready genomic DNA from plant leaf samplesFast: PCR-ready DNA in about 8 minutes for most samplesSimple: No bead-beating, freezing, or grinding of plant leaf materialIncreased Yields: No centrifugation or spin columns used which reduce yieldsCompatible: Extracted DNA is compatible with both real-time and end-point PCRSafe: Non-toxic reagents used throughout the procedure
Biotium AccuGreen™ Broad Range dsDNA Quantitation Kit (100 assays)
The AccuGreen™ Broad Range dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL. The assay has a green fluorescence with an excitation/emission of 500/530 nm (with DNA). This trial size kit has sufficient reagent for 100 assays.
Macherey-Nagel NucleoSpin 96 Blood QuickPure, Efficient DNA Purification, Silica-Membrane Technology, 96-Well Format
The NucleoSpin 96 Blood QuickPure kit offers an efficient solution for rapid DNA purification from various blood samples, utilizing advanced silica-membrane technology. This kit minimizes hands-on time while ensuring high-purity DNA suitable for sensitive applications like PCR. With a capacity of 384 preps, it is designed for streamlined processes and high yields, setting it apart from other products.
Qiagen, Inc. QIAGEN PLASMID PLUS MAXI KIT (
QIAGEN Plasmid Plus Maxi Kit (25)
Beckman Coulter GENFIND V3 REAGENT KIT - 384 P
- High-throughput genomic DNA isolation kit
- Utilizes SPRI paramagnetic bead-based technology
- Efficient recovery of high-quality DNA
- Compatible with various sample types including whole blood
- Supports multiple anticoagulants including citrate and EDTA
- 384 preps for 200 µL samples, 192 preps for 400 µL samples
- High quality, intact genomic DNA output
- Applicable for PCR, genotyping, and Next-Generation Sequencing
- Manual and automated workflow options available
- Compatible with Beckman Coulter Biomek liquid handlers
- Liquid format for ease of use
- High throughput capability
- Research use only
- Minimizes hands-on time and pipetting errors
- High DNA recovery rate with reduced PCR inhibitors
