DNA Extraction and Purification
Zymo Research Corporation EZ Nucleosomal DNA Prep Kit, 20 preps
Atlantis dsDNase is a double-strand DNA specific endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’- phosphate and 3’-hydroxyl termini. Atlantis dsDNase digestion yields very homogeneous populations of core nucleosomes and purification of the nucleosome-associated DNA is performed using Zymo Research’s proven Fast-Spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 45 minutes.
Zymo Research Corporation EZ-96 DNA Methlyation-Direct™ Kit (Shallow-Well) (2 x 96 Rxns)
The increased sensitivity of this kit makes it possible to amplify bisulfite converted DNA from as few as 10 cells or 50 pg DNA. Like the EZ DNA Methylation-Gold™ Kit, DNA denaturation and bisulfite conversion processes are combined into a single step. All kits streamline the three step process of bisulfite conversion of nonmethylated cytosine in DNA into uracil. In addition the methylation kits share innovative in-column desulphonation technology that eliminates otherwise cumbersome DNA precipitation steps while ensuring researchers consistent results every time. The kits have been designed to minimize template degradation, loss of DNA during treatment and clean-up, and to provide complete conversion of unmethylated cytosines. Recovered DNA is ideal for PCR amplification for downstream analyses including restriction endonuclease digestion, sequencing, microarrays, etc.
![Zymo Research Corporation ZymoBIOMICS DNA 96 MagBead Kit (2 x 96 preps)Includes: [D4306] ZymoBIOMICS 96 MagBead Kit x 1, [S6002-96-4] ZR BashingBead Lysis Racks (0.1mm & 0.5 mm) 2 pack x 1[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D4302-ZYMOBIOMICS-96-MAGBEAD-DNA-K.JPG-250.jpg)
Zymo Research Corporation ZymoBIOMICS DNA 96 MagBead Kit (2 x 96 preps)Includes: [D4306] ZymoBIOMICS 96 MagBead Kit x 1, [S6002-96-4] ZR BashingBead Lysis Racks (0.1mm & 0.5 mm) 2 pack x 1[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
New England Biolabs, Inc. NEBNext Library Quant Kit for Illumina – 500 reactions
The NEBNext Library Quant Kit components have been optimized to deliver significant improvements to qPCR-based library quantitation for Illumina sequencing. The kit contains primers which target the P5 and P7 Illumina adaptor sequences, and a set of four high-quality, pre-diluted DNA standards to enable reliable quantitation of diluted DNA libraries between 150–1000 bp.
Zymo Research Corporation Quick-DNA™ Viral 96 Kit (4 x 96 Preps)
Quick recovery of viral DNA from a wide range of sources using Zymo-Spin plate technology. Plate design allows DNA to be eluted at high concentrations into minimal volumes. Eluted DNA is suitable for PCR, Southern blotting, and restriction endonuclease digestion.
Norgen Biotek Corp Oligo Clean-Up/Concentrat Kit
This kit provides a rapid spin column procedure for the purification and concentration of up to 10 µg of oligonucleotides of various sizes. The kit is used for the purification of synthesized oligos, as well as the clean-up of oligos from various upstream enzymatic reactions such as ligation, Poly(A) tailing and end-labeling. Single or double stranded DNA or RNA oligos larger than 10 bp can be purified with this kit (not recommended for the removal of PCR primers). Oligos of up to 1000 bp have been tested with this kit.This kit purifies oligos from other reaction components including proteins, buffers and nucleotides without the use of phenol, chloroform, alcohol precipitation or urea PAGE extraction. The kit provides a high quality product with up to 90% recovery. Purified oligos can be used in a number of downstream applications such as end-point or quantitative reverse transcription PCR and RNAi studies.
Research Products International Corp EZ-96 DNA Methylation-Lightning MagPrep, 4 x 96 Preps
The EZ-96 DNA Methylation-Lightning MagPrep Kit is the fastest magnetic bead-based DNA bisulfite conversion kit for high-throughput and automated methylation analysis. No preparation is necessary with the ready-to-use conversion reagent - simply add this unique reagent to a DNA sample and incubate the reaction for about one hour. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. This magnetic bead-based bisulfite conversion kit produces the highest yields of bisulfite-converted DNA that is ideal for PCR, arrays, library preps, and Next-Generation sequencing.
High-throughput procedure for automated rapid and complete bisulfite conversion of DNA for methylation analysis.
Ready-to-use conversion reagent is added directly to DNA.
High-yield, converted DNA is ideal for PCR, Methylation Specific PCR (MSP), arrays, library preps, Next-Generation sequencing, etc.
INTACT GENOMICS INC RNA Cleanup Kit 100 Preps
The Intact Genomics IG RNA Cleanup Kit purifies up to 50 µg of RNA. Has a rapid and simplified workflow with a single wash buffer. Efficiently purify RNA ≥ 25 nt (a simple modification enables purification of RNA ≥ 15 nt;)Option to Elute in 20-50 µL for more concentrated RNA than the standard 100 µL; Standard 70-100% RNA recovery; Customizable buffer options for difficult reactions; Purified RNA is ready for use in a wide variety of downstream applications including: RT-PCR, RNA library prep for NGS, formation of RNP complexes for genome editing, microinjection, RNA labeling, and transfection.This kit is recommended for use following the IG sgRNA Synthesis Kits to remove proteins, salts and most unincorporated nucleotides. The Intact Genomics RNA Cleanup Kit has a simplified workflow and is ideal for cleanup and concentration of RNA after enzymatic treatments such as DNase I, Proteinase K, labeling, capping or in vitro transcription (IVT).
Epoch Life Science Inc GenCatch™ Anion Exchange DNA Maxi Columns (50/PK)
Ion exchange columns for transfection grade plasmid DNA purification. Anion-exchange technology. 1000 ug (1 mg) plasmid DNA binding capacity. Gravity flow. Compatible with third party buffer sets.
Zymo Research Corporation Genomic DNA Wash 1, 25 ml
Genomic DNA Wash 1 (25 ml). Sold separately or as part of a nucleic acid purification kit from Zymo Research.
Zymo Research Corporation ZymoPURE™ Wash 2 (Concentrate) (10 ml)
ZymoPURE™ Wash 2 Concentrate (10 ml). Assists in washing ZymoPURE™ columns for subsequent purification of nucleic acid.