RNA Preparation and Purification
Norgen Biotek Corp RNA/Protein Purification Plus Kit, Spin column method, 30-35 μg RNA yield (10 mg tissue), 100-150 μg protein yield (10 mg tissue), 50 preps
The RNA/Protein Purification Plus Kit provides a rapid and efficient spin column-based method for the sequential isolation and purification of total RNA and proteins from biological samples without hazardous reagents. This kit enables researchers to conduct analyses of both the transcriptome and proteome from difficult-to-obtain samples, ensuring high-quality recovery of macromolecules.
- Rapid protocol with hands-on time under 30 minutes
- Efficient isolation of total RNA, including all sizes like mRNA and miRNA
- Compatible with various sample types: animal cells, tissues, blood, bacteria, yeast, fungi, and plants
- Includes dedicated gDNA removal technology for DNA-free RNA
- Yield of 30-35 μg RNA and 100-150 μg protein from 10 mg hamster liver
- Suitable for diverse downstream applications in RNA and protein analysis
- Shelf life of 1 year after shipment
- 50 preps included in the kit
Research Products International Corp Direct-zol RNA MicroPrep w/Zymo-Spin IC Columns, Capped, 50 Preps
The Direct-zol RNA MicroPrep Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per prep) of high-quality RNA directly from samples in TRI Reagent or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol method assures unbiased recovery of small RNAs including miRNA. The procedure is easy. Simply apply a prepared sample in TRI Reagent directly to the Zymo-Spin Column and then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis.
Epoch Life Science Inc GenCatch™ Plant RNA Purification System (50 preps) Uniquely formulated buffers, shearing tubes, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify total RNA from plant (and fungal) samples. Comes with shearing tubes for simple and fast homogenization and filtration of tissue. Total RNA with a molecular size greater than 200 nucleotides (which excludes smaller RNA such as 5S and 5.8S RNA, and tRNA) can be isolated. Silica membrane technology. Up to 100 ug yield. 50 ul elution volume. 100 mg sample size. Additional buffer provided for handling plant tissue that contains sticky secondary metabolites. RNA isolated can be used for a variety of molecular manipulations such as Northern blotting, RT-PCR, PolyA+ RNA selection, cDNA synthesis, Primer extension, in vitro translation, etc. No phenol/chloroform extraction nor ethanol precipitation required. Used by labs around the world. 18 month shelf life.
Biochain Institute Inc Universal RNA - Mouse Normal Tissues, 2x100 ug/PK
BioChain's Universal RNA is the total RNA prepared with diversified tissues to ensure the broadest coverage of genes. It can be used as controls for microarray study. The Universal RNA is made on an industrial scale to minimize the lot-to-lot variation.
Biochain Institute Inc Total RNA - Monkey (Rhesus) Normal Tissue: Liver, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Biochain Institute Inc Total RNA - Monkey (Cynomolgus) Normal Tissue: Liver, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Research Products International Corp RNA Clean & Concentrator-5 w/Zymo-Spin IC Columns, Capped, 200 Preps
The RNA Clean & Concentrator kits are RNA clean up kits that provide a simple and reliable method for the rapid preparation of high-quality RT-PCR-ready, DNA-free (R1013, R1014) RNA. This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt). The procedure is easy: Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA. The RNA can be eluted from the Zymo-Spin IC Column in as little as ≥ 6 µl of RNase-free water. The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.
NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
Norgen Biotek Corp Plasma/Serum RNA Purification Maxi Kit
This kit provides a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating and exosomal RNA using a two column method. This kit can purify RNA from fresh or frozen serum or plasma samples prepared from blood collected on either EDTA or Citrate, from volumes ranging from 2 mL to 5 mL. Plasma samples prepared from blood collected on heparin should not be used.The first column will handle the large volume input of bodily fluids that is followed by a concentration on a mini column for a final elution of 50 µL to 100 µL. All components for the purification and concentration are provided in one convenient and fast kit for the easy processing of large input volumes of bodily fluids.The purified plasma/serum RNA is fully compatible with all downstream applications including NGS, and more.
Zymo Research Corporation Quick-RNA Fungal/Bacterial MicroPrep™ Kit (50 Preps)
Ultra-high density ZR BashingBeads™ are included for sample homogenization coupled with a robust buffer system, delivering total RNA (including small/microRNAs). The Zymo-Spin™ IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin™ IC Column efficiently binds total RNA.RNA is eluted in as little as 6 l and is suitable for subsequent procedures including RT-qPCR.