RNA Preparation and Purification
New England Biolabs, Inc. NEBNext Low-bias Small RNA Library Prep Kit - 24 reactions
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 ng - 1,000 ng) or enriched small (0.05 ng - 5 ng) RNA. The kit's reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries. This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample. For optimal flexibility in multiplexing, the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5), which must be purchased separately; 480 indices are available.
- Generates libraries with the lowest bias among commercially available kits
- Fastest workflow (~3.5 hours) for single-day libraries
- Multiplexable with up to 480 UDIs
- Simplified, gel-free workflow
- 18-month shelf life
New England Biolabs, Inc. NEBNext Low-bias Small RNA Library Prep Kit - 96 reactions
NEBNext Low-bias Small RNA Library Prep Kit reliably generates high-quality NGS libraries from total (0.5 ng - 1,000 ng) or enriched small (0.05 ng - 5 ng) RNA. The kit's reagents and workflow together minimize the amount of bias exhibited by the resulting libriaries. This means that the libraries are more accurately reflective of the various small RNA species and their relative proportions in the sample. For optimal flexibility in multiplexing, the NEBNext Low-bias Small RNA Library Prep Kit is designed to work with the NEBNext LV Unique Dual Index Primers (Sets 1-5), which must be purchased separately; 480 indices are available.
- Generates libraries with the lowest bias among commercially available kits
- Fastest workflow (~3.5 hours) for single-day libraries
- Multiplexable with up to 480 UDIs
- Simplified, gel-free workflow
- 18-month shelf life
Zymo Research Corporation ZymoBIOMICS™ RNA Mini Kit (50 preps)
The ZymoBIOMICS RNA Miniprep Kit is designed for purifying RNA from a wide array of sample inputs that is ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae). The procedure uses Zymo-Spin Column technology that results in high-quality total RNA (including small RNAs 17-200 nt) that is free of PCR inhibitors and is ready for RT-PCR, hybridization, sequencing, etc. DNase I included.
Macherey-Nagel NucleoSpin RNA Plus XS, 50 preps
NucleoSpin RNA Plus XS (50) 50 preps for the isolation of total RNA - NucleoSpin RNA Plus XS Columns, NucleoSpin gDNA Removal XS Columns, Collection Tubes, buffers
Omega Biotek Inc NGS Workflow PCR Clean Up Mag Bind Total Pure NGS
The Mag-Bind TotalPure NGS kit efficiently purifies PCR products for next-generation sequencing, removing impurities with high recovery rates. It includes all necessary components for processing 5-100μL PCR volumes and is compatible with major sequencing platforms.
New England Biolabs, Inc. 3'-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog – 1 umol
Blocking of the 3' -hydroxyl of m7G with 3' -0-Me assures that the capped transcripts are homogeneous. The 3' - hydroxyl of the non-methylated G is the only 3' - hydroxyl available for initiation. The 5' terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level. For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation, and a decrease in the formation of the initiation complex of mRNAs for protein synthesis. Certain prokaryotic mRNAs containing a 5 terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system. Also a cap requirement has been observed for splicing eukaryotic substrate RNAs. A method using E. coli RNA Polymerase primed with m7G(5' )ppp(5' )G or m7G(5' )ppp(5' )A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas.
Macherey-Nagel NucleoSpin RNA Clean-up XS, 50 preps
NucleoSpin RNA Clean-up XS (50) 50 preps for the clean-up of total RNA - NucleoSpin RNA XS Columns, Collection Tubes, buffers
Epoch Life Science Inc GenCatch™ Viral RNA Miniprep Kit (250 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify viral nucleic acids from cell-free samples such as plasma, serum, body fluid, urine, and cell culture supernatant. This system utilizes guanidine thiocyanate and 2-mercaptoethanol for their capabilities to inactivate RNase and denature proteins. Purified viral RNA is suitable for applications such as RT-PCR, Northern and RNase protection assays. This system is ideal for isolation of RNA from pathogenic viruses such as hepatitis C virus (HCV) for RT-PCR screening of clinical samples.
Epoch Life Science Inc GenCatch™ Viral RNA Miniprep Kit (50 preps) Uniquely formulated buffers, reinforced columns with collection tubes, along with protocols.
Provides a simple, fast and cost-effective method to purify viral nucleic acids from cell-free samples such as plasma, serum, body fluid, urine, and cell culture supernatant. This system utilizes guanidine thiocyanate and 2-mercaptoethanol for their capabilities to inactivate RNase and denature proteins. Purified viral RNA is suitable for applications such as RT-PCR, Northern and RNase protection assays. This system is ideal for isolation of RNA from pathogenic viruses such as hepatitis C virus (HCV) for RT-PCR screening of clinical samples.
Sigma Aldrich Fine Chemicals Biosciences SeqPlex RNA Amplification
The SeqPlex RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (ffPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow.
Apexbio Technology LLC HyperScribe™ All in One mRNA Synthesis Kit (ARCA, T7, poly(A))(Synonyms: HyperScribe™ mRNA Kit, HyperScribe™ ARCA Kit, T7 ARCA-poly(A) mRNA Kit, All-in-One mRNA Synthesis Kit), 25 rxns.
HyperScribe All in One mRNA Synthesis Kit (ARCA T7 poly(A)) is designed for rapid in vitro generation of capped and polyadenylated mRNA transcripts Utilizing T7 RNA polymerase this system incorporates Anti-Reverse Cap Analog (ARCA) a Cap0 analog co-transcriptionally at the 5 terminus to confer mRNA stability and enhance translational efficiency After template DNA removal via DNase I treatment Poly(A) polymerase adds a poly(A) tail at the 3 -end further stabilizing the transcript and increasing translation initiation efficiency This kit is employed in research involving translation systems antisense RNA RNA interference RNA vaccines structural RNA studies and ribonuclease assays
LAMDA BIOTECH INC Column-Pure RNA Cleaning Conc
A spin column-based system designed for RNA purification and concentration from various sources. Suitable for cleaning RNA samples from in vitro transcription reactions and DNase treatments.