RNA Preparation and Purification
New England Biolabs, Inc. NEBNext RNA Depletion Core Reagent Set with RNA Sample Purification Beads - 24 rxns
In RNA-seq, highly expressed transcripts with minimal biological interest, such as ribosomal RNA (rRNA) can dominate readouts and mask detection of more informative low-abundance transcripts. This challenge is amplified when working with sample types for which pre-designed RNA depletion kits are not available. The NEBNext RNA Depletion Core Reagent set provides a customized approach to deplete unwanted RNA from any organism, using probe sequences designed with the user-friendly NEBNext Custom RNA Depletion Design Tool. The efficient RNase-H-based workflow, and close tiling of probes designed using the online tool, enables effective depletion from both low- and high-quality RNA, with a broad range of input amounts. The kit is also available with RNAClean beads.
Bioworld bioZOL™ - A animal total RNA Isolation, 100 mL
bio-WORLD's bioZOLTM-A RNA Isolation Kit is a single phase solution designed specifically for the isolation of pure, intact RNA from animal tissue and cells in less than 90 minutes. Kit utilizes guanidine thiocyanate and β-mercaptoethanol, two of the most potent inhibitors of RNase, to effectively disrupt cellular structure, while inactivating RNase released from membrane-bound organelles. Total RNA is free of protein and DNA and can be used for cDNA synthesis, Northern blotting and in vitro translation. The procedure eliminates the need for lengthy ethanol precipitations and cesium chloride gradients.Haz Mat fee applies
New England Biolabs, Inc. NEBNext Magnesium RNA Fragmentation Module – 200 reactions
Fragmentation of RNA can be performed by enzymes, heat alone, or exposure to divalent cations at elevated temperature. This kit provides highly efficient and tunable RNA fragmentation by incubation with Magnesium ions at 94°C.
