Miscellaneous Molecular Biology Reagents
Enzo Life Sciences FLUOR DE LYS developer I concentrate (300 µl)
An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS substrate. FLUOR DE LYS Developer is an essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS substrate. An appropriate FLUOR DE LYS family substrate, comprising an acetylated lysine side chain, is first incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes it so that, in a second step, treatment with the FLUOR DE LYS Developer produces a fluorophore. Typically, an ‘inhibitor stop’ will be included in the 1x Developer dilution (e.g. Trichostatin A) for class I and II HDACs, nicotinamide for sirtuins). Shipping: Shipped on Blue Ice. Long Term Storage: -80°C
eMolecules ETHYLENEBIS(OXYETHYLENENIT-25G
AstaTech | ETHYLENEBIS(OXYETHYLENENITRILO)TETRAACETIC ACID | 25g | H10668 | Purity: 98% | 67-42-5 | MFCD00004291 | MW: 380.35
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Rowley Biochemical Institute Inc PMA-PTA ACID SOLUTION
Phosphomolybdic-Phosphotungstic Acid Solution - 1 Pint - Part Number SO-919-PT - Also Aailable in 8oz Quart and Gallon from Rowley Biochemical
Sigma Aldrich Fine Chemicals Biosciences Trypsin Sequencing Grade from bovine pancreas |
Trypsin Sequencing Grade from bovine pancreas |
Bulldog Bio Inc CellCover Biomolecule Stabilizing Solution, 196 x 10ml
For parallel storage of proteins, RNA, and DNA in their cellular contextCellCover was developed for fast “one step” stabilization of biomolecules. Its non-toxic formulation instantaneously protects and preserves RNA and protein expression patterns in human and animal cell types. This includes cells found in solid tissues, such as tumors, and cultured cell clusters, such as organoids and spheroids. Labs can then analyze the treated cell’s DNA, RNA and proteins days or weeks later with almost no detectable change in levels, patterns or modifications.The rapid fixation of RNA and proteins is very important for expression patterns to remain unchanged. With other techniques, such rapid fixation is often at the expense of other factors such as molecule crosslinking by PFA (paraformaldehyde) or cell lysis by high-salt reagents that stabilize RNA. Not so with CellCover, which instantly fixes and stabilizes cells in their true state — maintaining RNA and protein expression.
Sigma Aldrich Fine Chemicals Biosciences DEBLOCKING REAGENT 3% TRICHLOR
Deblocking Reagent 3% Trichloroacetic Acid in Dichloromethane, 4L
HELLO BIO INC COMPOUND 21 DREADD AGONIST 21
Compound 21 (DREADD agonist 21) dihydrochloride (water soluble)