Gene Reporter Systems
Promega KDR Kinase Enzyme System
Recombinant human KDR (amino acids 789-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. KDR (or kinase insert domain receptor) is a growth factor receptor tyrosine kinase originally isolated from human endothelial cells.
Promega GTPase-Glo Assay, 10,000 assays
The GTPase-Glo Assay assesses the activities of GTPases, GAPs and GEFs, which are components of the GTPase cycle, by detecting the amount of GTP remaining after GTP hydrolysis in a GTPase reaction.
Promega Dual-Luciferase™ Reporter (DLR™) Assay System Passive Lysis 5X Buffer
Promotes rapid lysis of cultured mammalian cells without the need to scrape adherent cells or freeze-thaw. Lytic reagent of choice when processing cells for quantitation of firefly and Renilla luciferase activities using the Dual-Luciferase(R) Assay.
Promega FcgammaRIIa-H ADCP Reporter Bioassay, Core Kit 5X, 1 each
The FcgammaRIIa-H ADCP Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the potency and stability of antibodies and other biologics that specifically bind and activate FcgammaRIIa.
Promega EnduRen™ Live Cell Substrate
An automation-friendly, highly sensitive substrate for Renilla luciferase-based gene reporter and BRET applications.
Promega FAK Kinase Enzyme System
Recombinant human FAK was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. FAK is involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix.
Promega Kinase-Glo™ Max Luminescent Kinase Assay System
The Kinase-Glo™ Luminescent Kinase Assays are homogeneous non-radioactive systems for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction.
Promega Luciferase Assay System Components
For use with Promega Luciferase Assay Systems (see cat. no. PRE1500)
Promega NanoBRET TE HDAC DNA Bundle, 1 each
NanoBRET Target Engagement Assay Components are for use with all NanoBRET Target Engagement Assays.
Promega UDP-Glo Glycosyltransferase Assay + UDP-Galactose, 200 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega ADP-Glo™ Max ADP Detection Assay Kit
The ADP-Glo™ Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction.
Promega PKCι Kinase Enzyme System
Recombinant full-length human PKC iota was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C iota is a member of the protein kinase C family of serine-threonine kinases.
Promega JAK3 Kinase Enzyme System
Recombinant human JAK3 (amino acids 781-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. JAK3 is a member of the JAK family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction.
Promega Ultra Pure UDP-Glucose, 100mM, 250microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega Intracellular TE Nano-Glo Substrate/Inhibitor, 1,000 assays
The Intracellular TE Nano-Glo Substrate/Inhibitor is a supplement for NanoBRET™ Target Engagement Assays.