Gene Reporter Systems
Promega CHK1 Kinase Enzyme System
Recombinant full-length human CHK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK1 was originally identified in fission yeast to play a role in activation of the DNA damage checkpoint in the G2 phase of the cell cycle.
Promega CDK1/CyclinA2 Kinase Enzyme System
Recombinant full-length human CDK1 and CyclinA2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins. CDK1 is essential for completion of START, controlling event in the cell cycle required to initiate mitosis.
Promega p70S6K Kinase Enzyme System
Recombinant full-length human p70S6K was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues.
Promega cAMP-Glo™ Max Assay System
The cAMP-Glo™ Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels.
Promega Beta-Glo™ Assay System
The Beta-Glo™ Assay System is a homogeneous, luminescence-based method for quantitating beta-galactosidase expression in mammalian cells.
Promega Ultra Pure UDP-GlcNAc, 100mM, 250microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega PDGFRβ Kinase Enzyme System
Recombinant human PDGFR beta (amino acids 557-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR beta is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
Promega UDP-Glo Glycosyltransferase Assay + UDP-Glucose, 200 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega EPHA1 Kinase Enzyme System
Recombinant mouse EPHA1 (amino acids 569-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EPHA1 is a member of the Eph family of receptor tyrosine kinases that have been implicated in mediating developmental events.
Promega Luciferase Control RNA
Luciferase Control RNA is an uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail that produces functional luciferase when translated.
Promega UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA), 200 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega AMPK (A1/B1/G1) Kinase Enzyme System
Recombinant full-length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags. AMPK is an important energy-sensing enzyme that monitors cellular energy status.
Promega Tracer Dilution Buffer, 50ml
NanoBRET Target Engagement Assay Components are for use with all NanoBRET Target Engagement Assays.
Promega UDP-Glo Glycosyltransferase Assay + UDP-GlcNAc, 400 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega LCK Kinase Enzyme System
Recombinant full-length human LCK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LCK (p56lck) is a member of the src family of nonreceptor tyrosine kinases.