Gene Reporter Systems
Promega LYN B Kinase Enzyme System
Recombinant full-length human LYN B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. LYN is a 56kDa tyrosine kinase that is similar to mouse T-lymphocyte-specific tyrosine kinase p56lck, v-yes, v-fgr and v-src.
Promega SGK1 Kinase Enzyme System
Recombinant human SGK1 (amino acids 60-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SGK1 is a member of the serum- and glucocorticoid-induced protein kinase family.
Promega c-MER Kinase Enzyme System
Recombinant human c-MER (amino acids 578-872) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. c-MER is a tyrosine kinase and a member of the MER/AXL/TYRO3 receptor kinase family.
Promega PDGFRα Kinase Enzyme System
Recombinant human PDGFR alpha (amino acids 550-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. PDGFR alpha is a member of the PDGFR family of membrane receptors with intrinsic tyrosine kinase activity.
Promega FAK Kinase Enzyme System
Recombinant human FAK was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. FAK is involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix.
Promega Succinate-Glo JmjC Demethylase/Hydroxylase Assay, 1,000 assays
Succinate-Glo JmjC Demethylase/Hydroxylase Assay is a bioluminescent assay for detecting the activity of JumonjiC (JmjC) histone demethylases hydroxylases.
Promega MET Kinase Enzyme System
Recombinant human MET (amino acids 956-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MET is a proto-oncogene that encodes a transmembrane growth factor receptor.
Promega PKCι Kinase Enzyme System
Recombinant full-length human PKC iota was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Protein kinase C iota is a member of the protein kinase C family of serine-threonine kinases.
Promega ADP-Glo™ Kinase Assay + p70S6K Kinase Enzyme System
Recombinant full-length human p70S6K was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues.
Promega AXL Kinase Enzyme System
Recombinant human AXL (amino acids 473-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. AXL is a member of the receptor tyrosine kinase family, which has oncogenic potential and is implicated in human myeloid leukemia.
Promega Luciferase Assay System Components
For use with Promega Luciferase Assay Systems (see cat. no. PRE1500)
Abnova™ Secreted Alkaline Phosphatase Reporter Gene Assay Kit (Luminescence)
Used for the sensitive quantitation of SEAP in conditioned cell culture medium from transfected cells and functional study
Abnova™ Luciferase Reporter Gene Assay Kit
Bioluminescent assay for promoter regulated luciferase expression
Promega PCR Optimization Kit, 1 each
PCR Optimization Kit is a portfolio of preformulated high-quality buffers, 25mM MgCl2 and GoTaq MDx Hot Start Polymerase. Perform a series of short experiments to quickly survey which combination will yield optimal PCR performance specific for your assay.
Promega Dual-Luciferase™ Reporter (DLR™) Assay System Passive Lysis 5X Buffer
Promotes rapid lysis of cultured mammalian cells without the need to scrape adherent cells or freeze-thaw. Lytic reagent of choice when processing cells for quantitation of firefly and Renilla luciferase activities using the Dual-Luciferase(R) Assay.