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A wide variety of assay products for identifying and analyzing of proteins and their functions. Products utilize various detection methods and sample types and can be used for applications including medical, cellular, molecular, and protein research.
-Galactosidase or -D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains each constituting 1023 amino acids. It is very specific for D-galactose and requires K or Na and Mg2 to be fully active. -Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with -d-galactopyranosides.
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Bradford Protein Assay Kit Bradford method utilizes Coomassie Brillant Blue G-250 dye binding to an unknown protein and forming a complex which can be detected spectophotometrically at 595nm. Method of choice for protein quantitation. Specifications:Designed to quantitate 1 to 10µg/ml protein but can be scaled up to quantitate 10 to 100µg/ml simply by increasing the volume of the dye generating a standard curve in the 10 to 100µg/ml range.Components: Bradford Reagent 1L, NaCl 0.15M 200ml, BSA Standard (0.5 mg/ml) 2x1.5ml.
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Folin & Ciocalteu′s phenol reagent is most commonly used in the Lowry method for determining protein concentration.[7][6] It has also been used for the quantification of total phenolics.[12] In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750nm) or shoulder (660nm) are used to quantitate protein concentrations between 1-100 mg/ml while absorbance at 550nm is used to quantitate higher protein concentrations.
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Human/animal serum and plasma are the best sources of samples for biomarker identification. However, the presence of albumin in large amounts interferes with many assays and functional proteomic analysis. Serum albumin accounts for 50-70% of the total serum protein that causes loss of resolution for 1D and 2D electrophoresis. Effective removal of albumin using our reagent can increase sensitivity of the assay for many folds. Unlike a resin-based depletion kit (that only deals with microliter amounts of a sample), Minute albumin depletion reagent can handle a few microliters and up to several milliliters of samples. Over 90% of albumin can be removed in less than 5 minutes.
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