The Monod Bio LuxSit™ Pro kits enable scientists to easily build custom luminescence assays. Each kit includes (1) reaction buffer, (2) substrate, and (3) substrate reconstitution buffer. Plasmids encoding LuxSit™ Pro are sold separately.The LuxSit™ Pro family represents the world’s smallest and highest-performance de novo luciferases, available as full-length reporters or split complementation systems for detecting protein–protein interactions. The 13.5 kDa, ATP-independent enzyme is the first de novo luciferase designed using advanced machine learning, offering exceptional solubility, thermostability, and expression in both bacterial and mammalian cells. When combined with the LuxSit™ Pro Assay Buffer and Substrate, it produces bright, glow-type luminescence—several times more intense than Firefly or Renilla luciferases.Technical resources: monod.bio/access-luxsit-pro