The LuxSit™ s-Pro PPI Kit is a versatile two-component split reporter for studying protein–protein interactions (PPI) and developing custom biosensors. Each kit includes proprietary reagents: (1) reaction buffer, (2) substrate, and (3) substrate reconstitution buffer. Plasmids encoding LuxSit™ s-Pro are sold separately.The Monod Bio LuxSit™ Pro family represents the world’s smallest and highest-performance de novo luciferases. Available as full-length reporters or split complementation systems, the 13.5 kDa ATP-independent enzyme is the first de novo luciferase created using advanced machine-learning design. LuxSit™ Pro is monomeric, highly soluble, thermostable, and expressible in both bacterial and mammalian cells. When paired with the LuxSit™ Pro Assay Buffer and Substrate, it delivers glow-type luminescence several times brighter than Firefly or Renilla luciferases.Learn more at monod.bio