General Cell Based Assays
Amsbio LLC Oris Universal Cell Migration Assembly Kit - FLEX
Oris™ Universal Cell Migration Assembly Kit - FLEX
Amsbio LLC Oris Cell Migration Assay - Collagen /Coated
Oris™ Cell Migration Assay - Collagen I Coated
Eurofins Discoverx PathHunter eXpress IL1RL1/IL1RAP Dimerization Assay, 2pl Kit. (93-1067E3CP5M)
The PathHunter IL1RL1/IL1RAP Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-33) induced dimerization of IL1RL1/IL1RAP receptors. This product uses EFC technology. The cell line included overexpresses IL1RL1 target protein fused to PK alongside IL1RAP target protein fused to EA. Upon ligand-induced dimerization of IL1RL1/IL1RAP, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Signaling Technology Phospho-AMPK-alpha Thr172 A9S3O Mouse mAb BSA and Azide Free 100 ug
Phospho-AMPK-alpha Thr172 A9S3O Mouse mAb BSA and Azide Free 100 ug
Epigentek Inc EPIQUIK KIT
EpiQuik Hydroxymethylated DNAImmunoprecipitation (hMeDIP)Kit (24 reactions)
Cayman Chemical GGT asy Buffer 25mL
GGT assay buffer has been tested and formulated to work exclusively with Cayman’s γ-Glutamyl Transferase Activity Assay Kit (Item No. 702210).
Revvity Health Sciences Inc AlphaPlex 545 (Terbium) Human IL-17 Detection Kit, 100 assay points
AlphaPlex 545 (Terbium) Human IL-17 Detection Kit, 100 assay points
Dojindo Molecular Technologies Inc GSSG/GSH Quantification Kit (200 TESTS) is high reproducibility for detecting GSH, GSSG and total glutathione individually. Easy and simple procedure, takes 1-2 hours.
Glutathione is a tripeptide present in the body, involved in antioxidation, drug metabolism, as well as enzyme substrates of glutathione peroxidase, glutathione S-transferase, and thiol transferase, etc. Glutathione is usually present as reduced form (GSH), but GSH is converted into its oxidized form (GSSG) by stimulation such as oxidative stress. Therefore, the ratio of GSH and GSSG has been noted as an index of oxidative stress. The GSSG/GSH Quantification kit contains the Masking Reagent of GSH. The GSH can be deactivated in the sample by simply adding the Masking Reagent. Therefore, the GSSG is detected by measuring the absorption (λmax = 412 nm) of DTNB (5,5 Edithiobis (2-nitrobenzoic acid) ) using the enzymatic recycling system. Also, GSH can be determined by the quantity by subtracting GSSG from the total amount of glutathione. The kit is limited to quantifying GSH/GSSG concentration from 0.5 μmol/l to 50 μmol/l and GSSG concentration from 0.5 μmol/l to 25 μmol/l.
Dojindo Molecular Technologies Inc NAD/NADH Assay Kit-WST (100TESTS), enables quantification of the amount of total NAD+/NADH, NAD+ and NADH in cells and measurement of their ratio.
Recently, it has become clear that Sirtuin is linked to longevity and plays a role in NAD+ level regulation. Also Sirtuin has been recognized as a marker necessary to understanding biological states, such as obesity & diabetes, as well as cellular differentiation. Cell lysate from a cell culture can be easily prepared via Deproteinzation using the extraction buffer and filtration tubes found within this kit. Intracellular NADH levels can be quantified by heat treatment of cell lysate. Additionally, intracellular NAD+ levels can be determined by subtracting NADH levels from independently measured total NAD+/NADH levels. NAD/NADH Assay Kit-WST enables quantification of the amount of total NAD+/NADH, NADH and NAD+ in cells and measurement of their ratio. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
Revvity Health Sciences Inc HTRF Histamine Dynamic Detection Kit, 500 Assay Points
HTRF Histamine Dynamic Detection Kit, 500 Assay Points
Cayman Chemical GlycolysIs asy Cofactr 250uL
Source Active recombinant MERS-CoV C-terminal His-tagged spike glycoprotein S1 subunit expressed in HEK293 cells Amino Acids 18-725 Tag C-terminal His MW 79.9 kDa
Cayman Chemical Cel-Based asy DMF 2.5mL
An acid-sensitive fluorescent probe upon protonation it displays a shift in emission maxima from 410 nm to 510 nm upon excitation at 157 nm has been commonly used in photolithography
Cayman Chemical GSH MES Buffer2X 1ea
A DPP-9 inhibitor (IC50 0.0034 UM) selective for DDP-9 over DPP-8 DPP-4 DPP-2 FAP and prolyl endopeptidase (IC50s 0.6 >10 >5 10 and >10 UM respectively) induces LDH release from THP-1 and HL-60 leukemia cells at 10 UM