General Cell Based Assays
Eurofins Discoverx PathHunter eXpress HTR2C U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0289E3CP3L)
The PathHunter HTR2C eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C and EA-tagged β-Arrestin-2. Activation of HTR2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress GHRHR CHO-K1 GPCR Assay, 2-Plate Kit. (95-0076E2CP2M)
The cAMP Hunter GHRHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GRF) based activation of GHRHR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GHRHR, a naturally Gs coupled GPCR. Upon activation of GHRHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress C3AR1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0814E3CP0M)
The PathHunter C3AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C3a) based activation of C3AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C3AR1 and EA-tagged β-Arrestin-2. Activation of C3AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress IL7R/CRLF2 Dimerization Assay, 2pl Kit. (93-1019E13CP25M)
The PathHunter IL7R/CRLF2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. TSLP) induced dimerization of IL7R/CRLF2 receptors. This product uses EFC technology. The cell line included overexpresses IL7R target protein fused to PK alongside CRLF2 target protein fused to EA. Upon ligand-induced dimerization of IL7R/CRLF2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human IL-9 (IL9R/IL2RG) Dimerization Cell-Based Assay Kit (HEK 293)
The PathHunter Human IL-9 (IL9R/IL2RG) Stable Cell Line Dimerization Assay (HEK 293) measures ligand-induced dimerization of IL9R/IL2RG. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human RXRα Nuclear Receptor Protein Interaction Cell-Based Assay Kit (U2OS)
The PathHunter Human RXRα Nuclear Receptor Stable Cell Line Protein Interaction Assay (U2OS) contains a stable clonal cell line used to measure activated RXRα nuclear hormone receptor interaction with SRCPs. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical RXR RetInoId X receptr100uL
An inhibitor of PI3K and PI3KY (IC50s 24.5 and 33.2 nM respectively) greater than 300- and 120-fold selective for PI3K and PI3KY respectively over PI3Ka and PI3KB induces cell death in L-540 KM-H2 and L-428 cells at 10 UM induces cell cycle arrest at the G0/G1 phase in the same cells reduces tumor growth and vasculature induces tumor necrosis and induces the M1 phenotype in TAMs in an L-540 mouse xenograft model
Indigo Biosciences Human Adrenoceptor Alpha 1D (ADRA1D) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 384-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Alpha 1D reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1D. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Molecular Devices Neurotransmitter Uptake Assay, Explorer, Homogeneous, 96/384-Well
Molecular Devices' Neurotransmitter Transporter Uptake Assay Explorer Kit provides a homogeneous, fluorescence-based method to measure dopamine, norepinephrine, and serotonin transporter (DAT, NET, SERT) uptake activity in live cells. The no-wash format supports kinetic and endpoint reads and is optimized for high-throughput screening and for use with transfected or endogenously expressing cells.
- Homogeneous no-wash assay format.
- Fluorescent substrate for DAT, NET, and SERT uptake.
- Five lyophilized reagent vials, each sufficient for one 96- or 384-well plate.
- Compatible with bottom-read fluorescence microplate readers and kinetic read modes.
- Store reagents at -20°C; dye solution stable up to eight hours at room temperature or up to five days when aliquoted and frozen.
Eurofins Discoverx cAMP Hunter™ Human GPR109A Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human GPR109A Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR109A (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human c-Ret-GFR alpha 2 Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human c-Ret-GFR alpha 2 Stable Cell Line Functional Kinase Assay (U2OS) measures c-Ret-GFRα2 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress HTR2C U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0289E3CP3M)
The PathHunter HTR2C eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C and EA-tagged β-Arrestin-2. Activation of HTR2C stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Mouse ADORA2B (A2B) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse ADORA2B (A2B) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure ADORA2B (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
TRIBIOSCIENCE INC Catalase Assay 100 Tests
Tribioscience catalase Assay Kit provides a rapid, simple, reproducible, and sensitive tool for catalase activity determination in plasma, serum, urine, and other samples. The catalase activity assay uses enzyme reactions to produce color and fluorescence with the probe. The color intensity of the reaction at 570 nm or fluorescence intensity at λex/em = 530/590 nm is directly proportional to catalase concentration in the sample. The fluorometric assay is more sensitive than colorimetric assay. APPLICATIONS Direct Assays - Catalase enzyme activity in serum, plasma, urine, and other bio-samples. KEY FEATURES Flexible - Suitable for colorimetric and fluorometric methods. Accurate - Use 25 µL samples, detection ranges are 1.95-31.25 U/mL in 96-well plate the assay. Simple and high-throughput - Two-steps procedure - just load-incubate-adding-incubate-read. Kits can be used as a robust method.