General Cell Based Assays
Revvity Health Sciences Inc HTRF pAb Anti-Mouse IgG XL665-Conjugate, 5,000 Assay Points
HTRF pAb Anti-Mouse IgG XL665-Conjugate, 5,000 Assay Points
Revvity Health Sciences Inc HTRF Streptavidin XLent-Conjugate, 5,000 Assay Points
HTRF Streptavidin XLent-Conjugate, 5,000 Assay Points
Revvity Health Sciences Inc HTRF IP-One Gq Detection Kit, Standard, 1 Vial
HTRF IP-One Gq Detection Kit, Standard, 1 Vial
Eurofins Discoverx cAMP Hunter eXpress ADRB3 DLD1 GPCR Assay, 2-Plate Kit. (95-0182E13CP25M)
The cAMP Hunter ADRB3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB3 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB3, a naturally Gs coupled GPCR. Upon activation of ADRB3, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Mouse HCRTR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse HCRTR1 β-Arrestin Stable Cell Pool Assay (CHO-K1) contains a stable engineered cell pool used to measure mHCRTR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell pool vials, all required culture reagents for maintaining the cells in continuous culture, and detection reagents for running the assay.
Eurofins Discoverx PathHunter eXpress GPR109A CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0301E2CP2M)
The PathHunter GPR109A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. nicotinic acid) based activation of GPR109A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR109A and EA-tagged β-Arrestin-2. Activation of GPR109A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Mouse IL-31 ASY
The Meso Scale Discovery U-PLEX Mouse IL-31 Assay Kit quantifies mouse interleukin 31 (IL-31) in serum plasma and other samples This electrochemiluminescent immunoassay uses MSD s SULFO-TAG labels and multi-array technology for sensitive detection of IL-31 at sub-picogram levels It includes a 96-well plate with capture antibody detection antibody with SULFO-TAG calibrators and diluent The assay runs on MSD s SECTOR Imager providing results in 3 5 hours ensuring reliable quantification of mouse IL-31
Meso Scale Discovery R-PLEX Human IRS-1 ASY
The R-PLEX Human IRS-1 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures human IRS-1 in cell and tissue lysates It utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay has high specificity and low cross-reactivity It includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker reagents and calibrator The kit can test up to 80 samples in duplicate and has a testing time of 2 5 hours It is for research use only
Eurofins Discoverx PathHunter eXpress EDNRA CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0257E2CP0M)
The PathHunter EDNRA eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-1) based activation of EDNRA GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDNRA and EA-tagged β-Arrestin-2. Activation of EDNRA stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Detection Kit, 550 (100 plates), 40,000 dp (384-well). (93-0001L)
The PathHunter Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cell lines or with custom cell lines made using ProLabel/PK expression vectors. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 100 plates (96/384 well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0001l.
AURORA BIOLABS LLC Cov-19 NP Rabbit Binding Assay, 384 rxn
SARS-CoV-2 Nucleocapsid protein (NP) is one of the core components of SARS-CoV-2 virus. It forms a complex with viral genomic RNA in a helical symmetrical structure and plays a key role in the process of virus replication and assembly. Since NP is abundantly expressed during infection, it can be used as an important diagnostic marker for COVID-19 and also can be used as a potential drug target or developing vaccines. Terbium-labeled anti-Tag1 antibody serves as fluorescence donor, that binds to the His-Tagged NP. If a test rabbit antibody binds to NP, fluorescence- labeled anti-rabbit antibody (fluorescence acceptor) will be brought in close proximity with the fluorescence donor. Excitation of Terbium (340 nm) generates fluorescence resonance energy transfer (FRET) to the fluorescence-labeled acceptor, which consequently fluoresces at 665 nm (figure below). Thus, the test antibody binding to NP can be quantitively measured by calculation of the fluorescent ratio of 665 nm/620 nm.
Eurofins Discoverx PathHunter eXpress INSRb Functional Assay, 10-Plate Kit. (93-0466E3CP16L)
The PathHunter eXpress INSRb Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated INSRb dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged INSRb and an EA-tagged SH2 domain protein. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human Total eNOS Detection Kit, 500 Assay Points
The Total eNOS kit is designed to monitor the expression level of cellular eNOS (endothelial nitric oxide synthase), and can be used as a normalization assay for the Phospho-eNOS (Ser1177) kit.
Eurofins Discoverx cAMP Hunter™ Human RXFP4 Gi Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human RXFP4 Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure RXFP4 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TORC3 (CRTC3) Nuclear Translocation Cell-Based Assay Kit (U2OS)
The PathHunter Human TORC3 (CRTC3) Stable Cell Line Nuclear Translocation Assay (U2OS) contains a stable clonal cell line used to measure CRTC/CREB pathway activation via nuclear translocation of TORC3. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.