General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress GCGR CHO-K1 GPCR Assay, 10-Plate Kit. (95-0042E2CP2L)
The cAMP Hunter GCGR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. glucagon) based activation of GCGR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GCGR, a naturally Gs coupled GPCR. Upon activation of GCGR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress DRD5 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0094E2CP2L)
The cAMP Hunter DRD5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dopamine) based activation of DRD5 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type DRD5, a naturally Gs coupled GPCR. Upon activation of DRD5, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences fatty Acid Uptake Kit suff
fatty acids are critical for many biological processes including energy metabolism and the synthesis of structural cellular components. The facilitated uptake of fatty acids presumably through fatty acid transport proteins is thought to be regulated at multiple levels. The dysregulation of fatty acid uptake may contribute to diseases such as obesity type 2 diabetes and hepatic steatosis.
Sigma Aldrich Fine Chemicals Biosciences D Lactate Assay Kit suffic
Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is therefore a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple direct and automation-ready procedures for measuring lactate concentration are very desirable.
Meso Scale Discovery R-PLEX Human hCG alpha ASY
The R-PLEX Human HCG Alpha Assay Kit from Meso Scale Diagnostics is an electrochemiluminescent immunoassay for measuring human chorionic gonadotropin alpha subunit (hCG ) in various biological matrices It uses MSD s SULFO-TAG labels and multi-array plate technology for sensitive detection The kit includes a 96-well plate with capture antibody detection antibody with ruthenium label calibrators and diluent Sensitivity is 0 05 mIU/mL with an upper limit of 1000 mIU/mL suitable for monitoring hCG levels and pregnancy testing
Meso Scale Discovery U-PLEX Human ApoA1 ASY
The Meso Scale Discovery U-PLEX Human ApoA1 Assay Kit is a multiplex immunoassay that measures Apolipoprotein A1 (ApoA1) levels in human serum plasma and cell culture supernatant samples This assay utilizes electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision The kit includes a 96-well plate coated with capture antibody detection antibody Read Buffer Calibrator Diluent and lyophilized U-PLEX Calibrator This quantitative immunoassay allows users to accurately determine ApoA1 concentrations within 90 minutes using as little as 25 L of sample
Abcam PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase)
PEPCK Assay Kit (Phosphoenolpyruvate Carboxykinase)
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Senescence Cells Histochem
The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for b-galactosidase activity at pH 6. b-galactosidase activity is detectable in senescent cells but not in quiescent immortal or tumor cells.
Abcam Adenosine 5'-monophosphate (AMP) disodium he x ahydrate, AMPK activator, 25G
MW 391.19 g/mol. Nucleotide involved in the reactions of cellular energy transfers. Acts as an activator of AMP-activated protein kinase (AMPK). Used as a substrate for enzymes such as AMP-thymidine kinase, AMP deaminase and 5'-nucleotidase.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress HTR2C (VGV) U2OS β-Arrestin GPCR Assay, 10pl Kit. (93-0932E3CP19L)
The PathHunter HTR2C(VGV) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C(VGV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VGV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VGV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NMBR CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0925E2CP2L)
The PathHunter NMBR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuromedin B) based activation of NMBR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged NMBR and EA-tagged β-Arrestin-2. Activation of NMBR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress KISS1R U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0927E3CP5L)
The PathHunter KISS1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. kisspeptin) based activation of KISS1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged KISS1R and EA-tagged β-Arrestin-2. Activation of KISS1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM4 Total Internalization Assay, 2pl Kit. (93-0902E3CP0M)
The PathHunter CHRM4 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CHRM4 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CHRM4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CHRM4 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.