General Cell Based Assays
Eurofins Discoverx PathHunter eXpress AGTR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0312E2CP0M)
The PathHunter AGTR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. angiotensin II) based activation of AGTR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTR1 and EA-tagged β-Arrestin-2. Activation of AGTR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR141 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0385E2ACP1M)
The PathHunter GPR141 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR141 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR141 and EA-tagged β-Arrestin-2. Activation of GPR141 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GALR1 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0228E2CP0M)
The PathHunter GALR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. galanin) based activation of GALR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GALR1 and EA-tagged β-Arrestin-2. Activation of GALR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PPYR1 (NPY4R) CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0233E2CP0M)
The PathHunter PPYR1 (NPY4R) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. pancreatic polypeptide) based activation of PPYR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PPYR1 and EA-tagged β-Arrestin-2. Activation of PPYR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress NMU1R U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0232E3CP0L)
The PathHunter nMU1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuromedin U) based activation of nMU1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged nMU1R and EA-tagged β-Arrestin-2. Activation of nMU1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR3 CHO-K1 β-Arrestin GPCR Assays, 2-Plate Kit. (93-0271E2CP2M)
The PathHunter CXCR3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CXCR3 and EA-tagged β-Arrestin-2. Activation of CXCR3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress ADRA1B CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0274E2CP0L)
The PathHunter ADRA1B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged ADRA1B and EA-tagged β-Arrestin-2. Activation of ADRA1B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Sigma Aldrich Fine Chemicals Biosciences D-2-HYDROXYGLUTARATE (D2HG) KT
D-2-Hydroxyglutarate (D2HG) Assay Kit
Eurofins Discoverx PathHunter eXpress CCRL2 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0333E2ACP1M)
The PathHunter CCRL2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19) based activation of CCRL2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCRL2 and EA-tagged β-Arrestin-2. Activation of CCRL2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc CELL MIGRATION ASSAY
The CytoSelect 24-Well Cell Migration Assay Kit contains twelve 8 um polycarbonate membrane inserts in a 24-well plate, which migratory cells are able to pass through. Quantify cells using the provided cell stain solution.
Cell Biolabs Inc Cellular Senescence Detection Kit (SA-Beta-Gal Staining), 50 assays
Senescent cells show common biochemical markers such as expression of an acidic senescence-associated ß-galactosidase (SA-ß-Gal) activity. While senescence has been characterized primarily in cultured cells, there is also evidence that it occurs in vivo. Cells expressing markers of senescence such as SA-ß-Gal have been identified in normal tissues. Cell Biolabs' Cellular Senescence Assay Kit provides an easy-to-use and efficient method to determine cellular senescence. SA-β-galactosidase catalyzes the hydrolysis of X-gal, which produces a blue color. Each kit provides sufficient quantities to perform up to 50 assays in 35 mm wells.
Eurofins Discoverx cAMP Hunter eXpress CCR7 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0070E2CP2L)
The cAMP Hunter CCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19) based activation of CCR7 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CCR7, a naturally Gi/Go coupled GPCR. Upon activation of CCR7, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.