General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CXCR6 Total Internalization Assay, 2pl Kit. (93-0886E3CP0M)
The PathHunter CXCR6 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR6 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CXCR6 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CXCR6 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR35 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0942E2CP2L)
The PathHunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR35 and EA-tagged β-Arrestin-2. Activation of GPR35 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress PTHR1 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0118E2CP2M)
The cAMP Hunter PTHR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. parathyroid hormone) based activation of PTHR1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR1, a naturally Gs coupled GPCR. Upon activation of PTHR1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGER4 HEK 293 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0304E1CP0M)
The PathHunter PTGER4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER4 and EA-tagged β-Arrestin-2. Activation of PTGER4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress mOPRK1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0480E2MCP0L)
The PathHunter mOPRK1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dynorphin A) based activation of mOPRK1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mOPRK1 and EA-tagged β-Arrestin-2. Activation of mOPRK1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Culture Kit-112, (92-3112G)
AssayComplete Cell Culture Kit-112 is an optimized media kit intended for use in culturing and propagating Eurofins DiscoverX cell lines. This kit contains the base medium, relevant serum, and pen/strep required to produce a full cell culture medium for optimal cell culture results. This product is provided as a 3-component kit which will produce approximately 550 mL of finished media. Please refer to cell line specific datasheet to determine the recommended cell culture kit. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress AVPR1A U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0587E3CP0L)
The PathHunter AVPR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. vasopressin) based activation of AVPR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AVPR1A and EA-tagged β-Arrestin-2. Activation of AVPR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CCR4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0193E2CP0M)
The PathHunter CCR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL22/MDC) based activation of CCR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR4 and EA-tagged β-Arrestin-2. Activation of CCR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CHRM4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0349E2CP0M)
The PathHunter CHRM4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM4 and EA-tagged β-Arrestin-2. Activation of CHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Adalimumab Bioassay Kit, 2-Plate. (93-0538B15-00131)
The PathHunter Adalimumab Bioassay Kit provides a robust, highly sensitive, and easy-to-use functional cell-based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, cell plating reagent, control agonist, detection reagents, and assay plates. The ready-to-use cryopreserved cells have been manufactured for single-use to ensure assay reproducibility, and faster implementation from characterization to lot release. This assay has been optimized and qualified with Humira (not supplied in the bioassay kit). Humira is a registered trademark of AbbVie, Inc. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress GLP1R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0300E2CP0L)
The PathHunter GLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of GLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRM1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0213E2CP0L)
The PathHunter OPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. met 5-enkephalin) based activation of OPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRM1 and EA-tagged β-Arrestin-2. Activation of OPRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR30 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0373E2ACP2M)
The PathHunter GPR30 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR30 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR30 and EA-tagged β-Arrestin-2. Activation of GPR30 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter eXpress OPRM1 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0107E2CP2L)
The cAMP Hunter OPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. met-enkephalin, morphine) based activation of OPRM1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OPRM1, a naturally Gi/Go coupled GPCR. Upon activation of OPRM1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.