General Cell Based Assays
ELABSCIENCE Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method) (E-BC-K261-M) 96 tests
Triglycerides (TG) can be hydrolyzed by lipoprotein lipase into glycerol and free fatty acids. Glycerol produces glycerol-3-phosphate and ADP under the catalysis of glycerol kinase (GK). Glycerol-3-phosphate produces hydrogen peroxide under the action of glycerol phosphate oxidase (GPO). In the presence of trinder’s reagent, hydrogen peroxide is catalyzed by peroxidase to produce quinones which is proportional to the content of TG.
Norgen Biotek Corp SERUM PROTEIN DEPLETION KIT
ProteoSpin™ Abundant Serum Protein Depletion Kitapid and efficient removal of abundant proteins from serum and plasma samplesProcess 10 samples in 30 minutesAllows for visualization of low abundance proteinsConvenient and affordable spin column protocolGeneric protocol allows for depletion from human and other animal samplesPurification is based on spin column chromatography that uses Norgen’s proprietary Silicon Carbide resin separation matrix
Eurofins Discoverx HitHunter cAMP Assay for Biologics, 2,500/10,000 dp (96-well/384-well). (90-0075LM25)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Biologic drugs with a protocol optimized for use in a 96-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 25 plates (96-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
TRIBIOSCIENCE INC Total Antioxidant Capacity (TAC)- CUPRAC Colorimetric Assay, 200 Tests
TribioScience’s Total Antioxidant Capacity Colorimetric Assay provides a quick, efficient, and sensitive method to measure TAC from cell lysate, plasma serum, urine, tissue homogenates, and food extracts. This assay is based on the reduction of Cu2+ to Cu+ by antioxidants like Trolox. The resulting Cu+ specifically forms a colored complex with a colorimetric probe. The color intensity at 490nm is proportional to TAC in the sample.
Sigma Aldrich Fine Chemicals Biosciences Glycerol Assay Kit sufficient for 200 colorimetric or fluorometric tests | 1KT
Glycerol Assay Kit sufficient for 200 colorimetric or fluorometric tests | 1KT
Enzo Life Sciences PROTEOSTAT Aggresome detection kit (1x100tests)
Robust, quantitative detection of aggresomes relevant to the study of neurodegenerative disease, liver disease and toxicology. Aggresomes are inclusion bodies of aggregated, misfolded proteins that form when the ubiquitin-proteasome protein-degradation machinery is overwhelmed. Typically, aggresomes form in response to cellular stress and provide a cytoprotective mechanism by isolating misfolded proteins, leading ultimately to their clearance by autophagy. Protein aggregate formation is furthermore a hallmark of a variety of human diseases, such as Alzheimer's, Parkinson's, Amyotrophic lateral sclerosis or alcoholic liver disease.The PROTEOSTAT.PROTEOSTAT. . Applications: Flow Cytometry. Fluorescence microscopy. Fluorescent detection. Contents: PROTEOSTATAggresome detection reagent . Hoechst 33342 Nuclear stain. Proteasome Inhibitor (MG-132). 10X Assay Buffer
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 500 Assay Points
HTRF Human & Mouse Phospho-SMAD2 (Ser465/467) Detection Kit, 500 Assay Points
Cell Biolabs Inc Total Cholesterol Assay Kit (Colorimetric), 192 assays
Cell Biolabs’ Total Cholesterol Assay Kit is a simple colorimetric assay that measures the amount of total cholesterol present in plasma, serum, tissue homogenates, or cell lysates in a 96-well microtiter plate format. The assay will detect total cholesterol (cholesteryl esters plus free cholesterol) in the presence of cholesterol esterase or only free cholesterol in the absence of the esterase enzyme. Each kit provides sufficient reagents to perform up to 192 assays, including blanks, cholesterol standards and unknown samples. Sample cholesterol concentrations are determined by comparison with a known cholesterol standard. Cholesteryl esters can be quantified by subtracting the free cholesterol values from the total cholesterol value.
Enzo Life Sciences DPPIV/CD26 Assay kit for biological samples (1x96wells)
Because DPPIV/CD26 protein levels may not accurately reflect the levels of active DPPIV enzyme, it is useful to measure DPPIV activity rather than performing DPPIV immunoassay. The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered .½-volume NBS white microplate (Prod. No. BML-KI571)
Abcam Protease Activity Assay Kit (Fluorometric - Red)
Protease Activity Assay Kit (Fluorometric - Red)
The product is subject to the following: Abcam Restricted Use Statement
Sigma Aldrich Fine Chemicals Biosciences Ammonia Assay Kit sufficient for 100 assays | 1KT
Ammonia Assay Kit sufficient for 100 assays | 1KT