General Cell Based Assays
Meso Scale Discovery R-PLEX Human ALK ASY
The R-PLEX Human ALK Assay QuickPlex 5-PL Kit from Meso Scale Diagnostics is a multiplex immunoassay for measuring ALK in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection on the QuickPlex SQ 120 instrument The assay takes about 3 hours and needs 12 5 L of sample with a detection range of 19 to 10 000 pg/mL The kit includes reagents for calibration controls diluent detection antibodies read buffer and plates with capture antibodies
Eurofins Discoverx PathHunter Human MIP-3β (CCR7) β-Arrestin Cell-Based Assay Kit (HEK 293)
The PathHunter Human MIP-3β (CCR7) β-Arrestin Stable Cell Line Assay (HEK 293) contains a stable clonal cell line used to measure CCR7 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR30 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR30 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR30 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Mouse DRD5 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Mouse DRD5 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure mDRD5 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical 15-PGDH Assay Buffer (1X), 12 mL
15-PGDH Assay Buffer (1X) has been formulated and tested to work with Cayman’s Assay Kits. Detailed instructions for its use are contained in the respective Cayman Assay Kit booklet.
Meso Scale Discovery R-PLEX Human BMP-5 ASY
The R-PLEX Human BMP-5 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures BMP-5 levels in human serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator diluent and lyophilized BMP-5 calibrator The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 10 000 pg/mL It can be used to measure BMP-5 levels in biological samples for research applications
Eurofins Discoverx PathHunter eXpress IL2RB/IL2RG/IL2RA Dimerization Assay, 2pl Kit. (93-1003E3CP0M)
The PathHunter IL2RB/IL2RG/IL2RA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-2) induced dimerization of IL2RB/IL2RG/IL2RA receptors. This product uses EFC technology. The cell line included overexpresses IL2RB target protein fused to PK alongside IL2RG target protein fused to EA and untagged ILR2A. Upon ligand-induced dimerization of IL2RB/IL2RG/IL2RA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical cGAS MoncLNal ANTIBDYClo 300ug
Immunogen: Full length human recombinant protein • Host: Mouse, clone 5G10 • Species Reactivity: (+) Human cGAS • Application(s): WB, IF, and IP
Eurofins Discoverx PathHunter eXpress GPR4 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0369E2ACP1M)
The PathHunter GPR4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR4 and EA-tagged β-Arrestin-2. Activation of GPR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
FOCUS BIOMOLECULES Biochemical Reagents, LXG6403, Lysyl oxidase inhibitor, CAS 315705-04-5, 98% by TLC/NMR (Conforms)
Biochemical Reagents, LXG6403, Lysyl oxidase inhibitor, CAS 315705-04-5, 98% by TLC/NMR (Conforms), LXG6403 is a potent (IC50 1.3 uM) and selective (3.5x over LOXL2 and no inhibition of LOX1) lysyl oxidase (LOX) inhibitor. Active against LOX in multiple cell lines (IC50 less than5 uM against MDA-MB-231, HCC143, Hs-578-T, and HCC1937). LXG6403 enhanced chemosensitivity of triple negative breast cancer cells to anthracycline, doxorubicin, cisplatin, and paclitaxel. It inhibited collagen crosslinking and deposition, increased doxorubicin penetration in cells and organoids, inhibited FAK signaling, and potentiated ROS/DNA damage axis in combination with cisplatin. LXG6403 was able to overcome doxorubicin resistance in the TM01278 TNBC PDX model.
Eurofins Discoverx PathHunter eXpress CCR4 Total Internalization Assay, 2pl Kit. (93-0845E3CP27M)
The PathHunter CCR4 Total Internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CCR4 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged CCR4 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the CCR4 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human AB total ASY
The Meso Scale Discovery R-PLEX Human Abeta Total Assay is an electrochemiluminescent immunoassay for quantifying total Abeta in human serum plasma and CSF It uses MSD s SULFO-TAG labels and multi-array technology for sensitive reproducible Abeta measurement The assay quantifies from 1 22 to 1000 pg/mL without cross-reactivity with other amyloid proteins It requires 50 L of sample and is compatible with MSD s SECTOR Imager 6000 for high throughput The R-PLEX Abeta Total Assay is a precise tool for Alzheimer s disease research
Eurofins Discoverx PathHunter Human GPR142 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR142 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR142 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery U-PLEX Mouse IL-17C ASY
The Meso Scale Discovery U-PLEX Mouse IL-17C Assay Kit is a multiplex immunoassay that enables simultaneous quantification of mouse interleukin 17C (IL-17C) in cell culture supernatants serum plasma and other biological matrices This assay utilizes MSD s proprietary electrochemiluminescent detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements The kit includes a 96-well plate pre-coated with capture antibodies detection antibodies and calibrators and controls needed to generate a standard curve and quantify IL-17C concentrations This assay allows for rapid reproducible quantification of IL-17C from mouse samples
Cayman Chemical MitoLite™ NIR FX690, 500 test
A mitochondrial dye for live or fixed cells; ex/em = 660/692 nm; has been used to determine subcellular localization of photosensitizers modified by polyethylene glycol in HepG2 cells