Protein Purification
STEMCELL Technologies EasySep™ Direct Human B Cell Isolation Kit, For processing: 100 mL whole blood processed
Isolate highly purified B cells directly from human whole blood without the need for density gradient centrifugation, RBC sedimentation or lysis. This platform provides fast and fully immunomagnetic negative selection.This cell isolation kit targets non-B cells for removal with EasySep™ Direct RapidSpheres™ and antibodies recognizing specific surface antigens. Unwanted cells are separated from desired cells using an EasySep™ magnet, and untouched target cells are then pipetted or poured into a new tube. Isolated cells can be used directly in downstream applications such as flow cytometry, culture or DNA/RNA extraction.
CUBE BIOTECH PureCube Fe-NTA MagBeads; 5 ml
PureCube Fe-NTA MagBeads were developed for the enrichment of phosphorylated proteins the affinity matrix is based on the spherical magnetic agarose beads, consisting og 6% cross-linked agarose. The material is highly porous to allow optimal protein interaction. Cross-linked agarose is also physically very stable, making it suitable for purification processes without deformation or destruction. The magnetic beads are very homogeneous in size with a medium particle diameter of 30um, yielding a high degree of reproducibility between individual purificaiton runs.
Bio Rad Laboratories ProteoMiner Protein Enrichment Small-Capacity Kit, 10 preps, Combinatorial peptide ligand library, 500 μL bead slurry per column, 20 μL settled bead volume
ProteoMiner Protein Enrichment Small-Capacity Kit, 10 preps, Combinatorial peptide ligand library, 500 μL bead slurry per column, 20 μL settled bead volume
Biotium GloMelt™ Thermal Shift Protein Stability Kit, with ROX, 2000 assays
GloMelt™ dye can be used to detect protein unfolding or measure thermal stability by performing a thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.
STEMCELL Technologies EasySep™ Direct Human Monocyte Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Monocyte Isolation Kit isolates functional, highly purified CD14+ monocytes directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture and other downstream applications.
Supply Solutions cOmplete™, EDTA-free Protease Inhibitor Cocktail, 20 tablets
Roche - Complete, EDTA-free, 20 tablets; Tablets provided in glass vials; vial of 20 tablets
STEMCELL Technologies EasySep™ Human Naïve CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Naïve CD4+ T Cell Isolation Kit is designed to isolate naive CD4+ T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes and dextran-coated magnetic particles. CD45RO+ cells are targeted for removal with a biotinylated anti-CD45RO antibody, and a bispecific Tetrameric Antibody Complex that recognizes biotin and dextran. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For even faster cell isolations, we recommend the new EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (17555) (https://www.stemcell.com/products/easysep-human-naive-cd4-t-cell-isolation-kit-ii.html) which isolates cells in just 11 minutes.
Zymo Research Corporation Zymo-Spin II-PX Columns (50 Pack)
Available as a refill for the ZymoPURE Plasmid Miniprep Kit. The versatile Zymo-Spin II-PX column can be used in microcentrifuges or on vacuum manifolds for the purification of plasmid DNA. The Zymo-Spin II-PX features durable polypropylene construction and a unique silica-based matrix that allows for purification of up to 100 µg of plasmid DNA in ≥ 25 µl eluate when used in combination with ZymoPURE Plasmid Prep buffers. Capacity is 800 µl.
Sigma Aldrich Fine Chemicals Biosciences Triton(TM) X-100 laboratory grade | 9002-93-1 | MFCD00128254 | 1L
Triton(TM) X-100 laboratory grade | 9002-93-1 | MFCD00128254 | 1L
STEMCELL Technologies EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II is designed to isolate CD4+CD25+ T cells from single-cell suspensions of splenocytes or other tissues by negative followed by positive selection. CD4+ T cells are first enriched by negative selection using the EasySep™ Mouse CD4+ T Cell Isolation Cocktail. CD25+ cells are then positively selected from the pre-enriched cells using EasySep™ Mouse CD25 Regulatory T Cell Positive Selection Cocktail.
Sigma Aldrich Fine Chemicals Biosciences RNase A from bovine pancre
Bovine pancreatic RNase A is a member of the RNase A protein superfamily. It is one of the most characterized proteins and is a kidney shaped basic protein. This protein is composed of 124 amino acids. In its native form RNase A exists as a homodimer.
Sigma Aldrich Fine Chemicals Biosciences Choline Oxidase from Arthr
The codA gene encodes the choline oxidase enzyme.