Protein Purification Reagents and Kits
Hampton Research CRYSTAL SCREEN NO08 185ML
HR2-910-08/Crystal Screen #08 (A8), 185 ml - 0.2 M Sodium citrate tribasic dihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 30% v/v 2-Propanol. Please note items from this supplier are non-returnable.
Hampton Research GRAS Screen 6, 1 mL, Deep Well block format
GRAS Screen 5 and 6 were developed by Hampton Research for the crystallization of proteins, including monoclonal antibodies.
STEMCELL Technologies RoboSep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD15 Positive Selection Kit is designed to isolate highly purified CD15+ cells from fresh whole blood by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD15 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD15 antigen is expressed on granulocytes and monocytes.This product replaces the EasySep™ Human Whole Blood CD15 Positive Selection Kit (Catalog #18681) and EasySep™ HLA Whole Blood CD15 Positive Selection Kit (Catalog #18681HLA), for even faster cell isolations.
STEMCELL Technologies EasySep™ "Do-It-Yourself" Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ "Do-It-Yourself" Selection Kit II is designed to select any cell type of interest with your own mouse IgG1 monoclonal antibody. Cells that express the surface antigen that is recognized by the mouse IgG1 monoclonal antibody are targeted for magnetic separation using EasySep™ Dextran RapidSpheres™ and the EasySep™ Magnet.This product replaces the EasySep™ "Do-It-Yourself" Selection Kit (Catalog #18098).
Hampton Research HR2-906-14/StockOptions Bis-Tris #14 pH 6.8, 185 ml
HR2-906-14/StockOptions Bis-Tris #14 pH 6.8, 185 ml. Please note items from this supplier are non-returnable.
Waters Corp Waters BioResolve CX pH Concentrate, 10X Buffer, pH 5.0 to 10.2 Gradient
This 10X buffer concentrate is optimized for creating a pH gradient for monoclonal antibody (mAb) charge variant separations. It is designed for use with BioResolve SCX columns.
- 10X buffer concentrate
- Optimized for creating a pH 5.0 to pH 10.2 gradient
- Designed for use in monoclonal antibody (mAb) charge variant separations
- Developed alongside BioResolve SCX columns
STEMCELL Technologies RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail, For processing: 200 mL bone marrow processed
The RosetteSep™ Human Bone Marrow Progenitor Cell Pre-Enrichment Cocktail is designed to pre-enrich progenitor cells by removing lineage-specific cells from whole bone marrow by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD3, CD11b, CD14, CD16, CD19, CD56, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The pre-enriched progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium.
STEMCELL Technologies RoboSep™ HLA Whole Blood T Cell Enrichment Kit with Filter Tips, For processing: 200 mL whole blood processed
The Complete Processing Kit for whole blood uses EasySep™ HLA T Cell Enrichment Kit to isolate human T cells from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with tetrameric antibody complexes recognizing non-T cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Direct Human PBMC Isolation Kit RoboSep™, For processing: 100 mL whole blood processed
The EasySep™ Direct Human PBMC Isolation Kit is designed to isolate highly purified peripheral blood mononuclear cells (PBMCs) from fresh whole blood, buffy coat, bone marrow, cord blood, or leukapheresis products by negative selection. Red blood cells (RBCs), platelets, and unwanted cells are targeted for removal with antibody complexes and magnetic particles and separated using an EasySep™ magnet. Untouched PBMCs are simply collected into a new tube and are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
STEMCELL Technologies RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, For processing: 5000 mL cord blood processed
The RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit is designed to pre-enrich hematopoietic progenitor cells from cord blood by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD3, CD11b, CD19 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium. Cells of interest can then be positively selected from the pre-enriched cell population using EasySep™ (e.g. EasySep™ Human CD34 Positive Selection).
STEMCELL Technologies EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit, For processing: 60 mL buffy coat or whole blood processed
The EasySep™ HLA Chimerism Whole Blood CD3 Positive Selection Kit is designed to isolate CD3+ cells from fresh whole blood or buffy coat by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD3 and dextran-coated magnetic particles. The cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD3 antigen is expressed on T cells and NKT cells.This product replaces the EasySep™ Human Whole Blood CD3 Positive Selection Kit (Catalog #18081) and EasySep™ HLA Whole Blood CD3 Positive Selection Kit (Catalog #18081HLA), for even faster cell isolations.
AdipoGen FTO human rec His
Protein. Human FTO aa 2-505 is fused at the N-terminus to a His-tag. Source E. coli. Endotoxin content <1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl. Purity >90% SDS-PAGE. FTO Fat mass-and obesity-associated gene is the responsible gene for mouse ‘fused toes’ mutation. An association between FTO genotype and type 2 diabetes has been confirmed. The presence of the FTO rs9939609 A-allele was found to be positively correlated with other symptoms of the metabolic syndrome, including higher fasting insulin, glucose, triglycerides, and lower HDL-cholesterol.
AdipoGen CTRP5 human rec His
Protein. Human CTRP5 aa 16-243 is fused at the N-terminus to a His-tag. Source E. coli. Endotoxin content <1EU/µg purified protein LAL test Lonza. Lyophilized from a 0.2µm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl. Purity >90% SDS-PAGE. CTRP5 C1qTNF-related protein 5 C1QTNF5 belongs to a highly conserved family of adiponectin paralogs. CTRP5 mediates activation of AMP-activated protein kinase AMPK in muscle and liver cells, thereby regulating glucose and lipid metabolism. Serum levels of CTRP5 are significantly higher in obese/diabetic animal models compared to normal controls. Furthermore, CTRP5 may be a putative biomarker for mitochondrial dysfunction. Defects in C1QTNF5 are a cause of late-onset retinal degeneration LORD.
Macherey-Nagel NucleoBond Xtra Maxi EF, 10 preps
NucleoBond Xtra Maxi EF (10) 10 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A