Protein Purification Reagents and Kits
STEMCELL Technologies RoboSep™ Mouse Memory CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Memory CD4+ T Cell Isolation Kit is designed to isolate pan-memory CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-memory CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD45R, CD45RB, CD49b, CD105 and TCRγ/δ) and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.Learn more about our next-generation EasySep™ mouse cell isolation kits (/easysep-15-minute-kits), featuring RapidSphere™ technology.
STEMCELL Technologies EasySep™ HLA B Cell Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ HLA B Cell Enrichment Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-B cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet and without the use of columns. Desired cells are poured off into a new tube and are ready for serology or flow cytometry crossmatch assays.
STEMCELL Technologies EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Non-Human Primate CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from fresh peripheral blood mononuclear cells by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This kit has been verified for use with rhesus and cynomolgus macaques.
STEMCELL Technologies RoboSep™ Human CD8+ T Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human CD8+ T Cell Enrichment Kit is designed to isolate CD8+ T cells from fresh or previously frozen peripheral blood mononuclear cells or ammonium chloride-lysed leukapheresis sample by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD8+ T cells and glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For even faster cell isolations, we recommend the new EasySep™ Human CD8+ T Cell Isolation Kit (17953) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-CD8-T-Cell-Isolation-Kit.aspx) which isolates cells in just 8 minutes.
STEMCELL Technologies RoboSep™ HLA B Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ HLA B Cell Enrichment Kit is designed to isolate B cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-B cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet and without the use of columns. Desired cells are poured off into a new tube and are ready for serology or flow cytometry crossmatch assays.
STEMCELL Technologies RoboSep™ Human Plasmacytoid DC Enrichment Kit with Filter Tips, For processing: 2 x 10^9 cells
The EasySep™ Human Plasmacytoid DC Enrichment Kit is designed to isolate plasmacytoid dendritic cells (pDC) from fresh peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-pDCs and dextran-coated magnetic particles. The labeled cells are separated using the EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies RoboSep™ HLA T Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ HLA T Cell Enrichment Kit is designed to isolate T cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-T cells and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube and are ready for serology or flow cytometry crossmatch assays.
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eMolecules Glutathione Disulfide-13C4,15N2 Ammonium Salt | Toronto Research Chemicals |618.580 | C20H32N6O12S2N[C@@H](CCC(=O)N[C@@H](CSSC[C@H](NC(=O)CC[C@H](N)C(O)=O)C(=O)[15NH][13CH2][13C](O)=O)C(=O)[15NH][13CH2][13C](O)=O)C(O)=O | 0.25mg | 483410836
Glutathione Disulfide-13C4,15N2 Ammonium Salt | Toronto Research Chemicals |618.580 | C20H32N6O12S2N[C@@H](CCC(=O)N[C@@H](CSSC[C@H](NC(=O)CC[C@H](N)C(O)=O)C(=O)[15NH][13CH2][13C](O)=O)C(=O)[15NH][13CH2][13C](O)=O)C(O)=O | 0.25mg | 483410836
Sigma Aldrich Fine Chemicals Biosciences Acetylcholinesterase Activ
The Acetylcholinesterase Activity Assay Kit (Colorimetric) provides a simple quick and direct procedure for measuring Acetylcholinesterase levels in a variety of samples such as serum plasma tissues or cell lysates / cell extracts. This assay is based on the Ellman method in which thiocholine produced by Acetylcholinesterase reacts with DTNB 5 5A¢-dithiobis (2-nitrobenzoic acid) to form a colorimetric product (412 nm) proportional to the Acetylcholinesterase activity present. This kit does not require weighing or mixing of multiple reagents. The Acetylcholinesterase activity is measured without the use of a calibration curve. The Acetylcholinesterase Positive Control in the kit can be used to screen for Acetylcholinesterase inhibitors. The detection range 10-600 units/liter of Acetylcholinesterase activity.The detection time 5-10 minutesAcetylcholinesterase ( AChE) is a serine hydrolase found at neuromuscular junctions and cholinergic brain synapses. AChE hydrolyzes choline es
BIOHIPPO AAV-EF1a-DO-EYFP-WPRE-hGH-polyA
Recombinant adeno associated virus expressing EYFP under the EF1a promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
BIOHIPPO AAV-CMV-FLEX-NLS-hSaCas9-NLS-3XHA-bGH-polyA-U6-SasgRNA(scramble)
Recombinant adeno associated virus expressing hSaCas9 SasgRNA (scramble) 3XHA under the CMV U6 promoter with WPRE and hGH polyA elements. The Cre dependent configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
Abcam Mitochondria/Cytosol Fractionation Kit, 25 Tests
Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells. - Cited in over 110 publications - Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction - Simple and easy procedure for fractionation - Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays
The product is subject to the following: Abcam Restricted Use Statement
Genscript Corporation GITR/TNFRSF18Biotin His A
GITR (glucocorticoid-induced tumor necrosis factor receptor) also known as AITR and TNFRSF18 is a 40 kDa transmembrane glycoprotein that functions in immune regulation GIRT is a receptor for TNFSF18 Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death Mediated NF-kappa-B activation via the TRAF2/NIK pathway