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Protein Purification Reagents and Kits

A variety of reagents, columns, kits, and associated products designed for high-yield purification of proteins.
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331 – 345 of 5,722 results
331

Cytiva HiScreen™ Capto™ S, Capto™ Q and Capto™ DEAE Prepacked IEX Columns

Designed for method optimization and parameter screening

Buffer 1M Acetic Acid, 1M NaOH, 8M Urea, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
Height (Metric) 100mm
Content And Storage 4° to 30°C
Diameter (English) Inner 0.3 in.
Bed Volume 4.7mL
Column Type Ion Exchange Column
Diameter (Metric) Inner 7.7mm
Quantity 1 x 4.7mL
Material Polypropylene
For Use With (Application) Modern large-scale manufacturers for fast, efficient and cost-effective capture and intermediate Protein purification, optimization and parameter screening
Max. Pressure 3 bar
Particle Size 45 to 165μm
Flow Rate <700 cm/hr.
Support Type Highly cross-linked Agarose with Dextran surface extender
Height (English) 3.93 in.
332

Cytiva HiTrap™ Con A 4B Columns

For separation and purification of glycoproteins, polysaccharides and glycolipids

Particle Size 45 to 165μm
Buffer Aqueous Buffer
Ligand Type Concanavalin A (13mg Con A/mL drained medium)
Height (Metric) 25mm
Content And Storage 4° to 8°C
pH Range 4 to 9
Product Type HiTrap Con A Sepharose 4B column
Column Type Ion Exchange Column
Material Polypropylene
For Use With (Application) Convenient separation and purification of Glycoproteins, Polysaccharides and Glycolipids
Support Type 4% Agarose
Max. Pressure 3 bar
Height (English) 0.98 in.
333

MilliporeSigma™ Novagen™ Ekapture™ Agarose

Intended for rapid affinity-based capture.

Type Agarose
Content And Storage 2°C to 8°C
334

MilliporeSigma™ Novagen™ S-protein Agarose
GSA_VA

Retains specifically S·Tag™ fusion proteins.

335

Cytiva GSTrap™ 4B Prepacked Columns: 16 x 25mm

For fast and simple purification of GST-tagged proteins

Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.62 in.
Bed Volume 5 mL
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
pH Range 4 to 13
Diameter (Metric) Inner 16 mm
Material Polypropylene
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function
Particle Size 45 to 165 μm
Ligand Type Glutathione
Flow Rate <20 mL/min.
Type Pre-packed Column
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Support Type 4% Highly Cross-linked Agarose
336

Cytiva GSTrap™ FF Columns: 7 x 25mm

Purifies GST-tagged proteins in one step

Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.27 in.
Bed Volume 1 mL
Description GSTrap FF columns are prepacked Glutathione Sepharose Fast Flow columns for fast, convenient, one-step purification of glutathione S-transferase (GST) tagged proteins.
pH Range 3 to 12
Diameter (Metric) Inner 7 mm
Material Polypropylene
For Use With (Application) For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract
Particle Size 90 μm
Ligand Type Glutathione and 10 carbon linker arm
Flow Rate <4 mL/min.
Type Pre-packed Column
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Support Type 4% Highly Cross-linked Agarose
337

Cytiva Capto™ Core 400

Designed for intermediate purification and polishing of viruses and other large biomolecules.

Buffer 1 M sodium hydroxide (NaOH), 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol
Height (Metric) 20 cm
Content And Storage 4°C to 30°C
pH Range 3 to 14
Final Product Type Chromatography Resin
Molecular Weight (g/mol) 400,000
Column Type Tricorn Column
Quantity Each
Expression System Virus
Particle Size 90 μm
Ligand Type Octylamine
Flow Rate 700 cm/hr.
Expression Competent Cell Retrovirus
Capacity 22 mg
Protein Form Virus
Product Type Agarose Chromatography Resin
dimMatrix Multimodal Resin
338

Cytiva™ Protein Select™ Resin

An affinity chromatography resin for purifying recombinant proteins using the self-cleaving Cytiva™ Protein Select™ tag.

339

Thermo Scientific™ CaptureSelect™ HRP Anti-AAVX Conjugate
GREENER_CHOICE

Thermo Scientific™ CaptureSelect™ HRP Anti-AAVX Conjugate includes a 14-kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds adeno-associated virus particles of multiple AAV serotypes.

Type Conjugate
Content And Storage Store in freezer (-5°C to -30°C).
Form Liquid
Concentration 0.5 mg/mL
For Use With (Application) Quantitation of multiple AAV capsids
Product Line CaptureSelect
340

Thermo Scientific™ Dynabeads™ CaptureSelect™ AAV9 Magnetic Beads
GREENER_CHOICE

Thermo Scientific™ Dynabeads™ CaptureSelect™ AAV9 Magnetic Beads are high-capacity, high-throughput magnetic affinity particles for the purification of naturally occurring and synthetic adeno-associated virus serotype 9 (AAV9) used for gene therapy applications.

Particle Size 4.5 μm
Ligand Type CaptureSelect™ AAV9 Affinity Ligand
Target AAV9 (Adeno-associated serotype 9)
Matrix Non-porous monodisperse superparamagnetic beads
Formulation 4 to 6% (v/v) Slurry
Concentration 30 mg beads/mL slurry, 1 mg contains approximately 1.3 x 107 beads
For Use With (Application) Magnetic isolation of AAV9 capsids
Product Line CaptureSelect
341

Thermo Scientific™ POROS™ Caprylate Mixed-Mode Cation Exchange Chromatography Resin

Thermo Scientific™ POROS™ Caprylate Mixed-Mode Chromatography Resin is designed to remove moderate to high levels of aggregates during downstream processing of therapeutic antibodies in flow-through mode.

Particle Size 50 μm
Shipping Condition Ambient, cold
Stationary Phase Caprylic Acid
Type Bulk Resin
Target Monoclonal antibodies
Matrix Poly(styrene-co-divinylbenzene)
Product Line POROS
342

Thermo Scientific™ CaptureSelect™ Biotin Anti-CD9 Conjugate
GREENER_CHOICE

Thermo Scientific™ CaptureSelect™ Biotin Anti-CD9 Conjugate consists of a 12–15 kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds with high affinity and selectivity to the tetraspanin marker CD9.

Shipping Condition Dry Ice
Ligand Type Recombinant single-domain antibody fragment (VHH ligand)
Target Tetraspanin CD9
Content And Storage Store in freezer (–5 to –30°C).
Reactivity Anti-tetraspanin CD9
Final Product Type Biotin Conjugate
Form Frozen Liquid
Formulation Phosphate-buffered saline (pH 7.4)
Label or Dye Biotin
Concentration 1 mg/mL
Product Line CaptureSelect
343

Cytiva Glutathione Sepharose™ High Performance Media

For high resolution purification of Glutathione S transferase (GST) tagged proteins

Particle Size 34 μm
Ligand Type Glutathione
Flow Rate <600 cm/hr.
Type Chromatography Media
Content And Storage 4°C to 30°C, 20% Ethanol
Matrix Highly crossed-linked agarose, 6%
Description Glutathione Sepharose High Performance is an affinity resin recommended for purifying glutathione S-transferase (GST)-tagged proteins when high resolution is the priority.
pH Range 3 to 12
Binding Capacity >Approx. 10 mg GST-tagged protein/mL resin
Support Type 6% Highly Cross-linked Agarose
344

Cytiva GSTrap™ 4B Prepacked Columns: 7 x 25mm

Use for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins. Cytiva Life Sciences™ GSTrap™ 4B Prepacked Columns: 7 x 25mm are prepacked with Glutathione Sepharose™ 4B

Ligand Type Glutathione
Flow Rate <4 mL/min.
Type Pre-packed Column
Content And Storage 4°C to 30°C, 20% Ethanol
Diameter (English) Inner 0.27 in.
Bed Volume 1 mL
Description GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
pH Range 4 to 13
Diameter (Metric) Inner 7 mm
Material Polypropylene
For Use With (Application) GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function.
Support Type 4% Highly Cross-linked Agarose
345

Cytiva MBPTrap™ HP Prepacked Columns
GSA_VA

Yield highly pure MBP-tagged recombinant proteins eluted in concentrated form and small volumes. Cytiva Life Sciences™ MBPTrap™ HP Prepacked Columns are prepacked with Dextrin Sepharose™ High Performance.

Buffer Aqueous Buffer
Height (Metric) 25mm
Content And Storage 2° to 8°C
Bed Volume 1mL
pH Range >7
Column Type Ion Exchange Column
Material Polypropylene
For Use With (Application) Purification of MBP tagged Proteins
Max. Pressure 3 bar
Particle Size 34μm
Ligand Type Dextrin
Product Type Dextrin Sepharose HP prepacked column
Support Type 6% Highly Cross-linked Agarose
Height (English) 0.98 in.