Protein Purification Reagents and Kits
Active Motif miRNA Target IP Kit
Active Motif's miRNA Target IP Kit was designed to identify the physical interactions of a miRNA with endogenous mRNA transcripts. It utilizes protein G-coupled magnetic beads and a pan-Ago antibody to efficiently immunoprecipitate miRNA/mRNA complexes that have associated with Ago1, Ago2 or Ago3 proteins as part of a RISC complex. Following their immunoprecipitation, the mRNA targets are purified and then amplified by qRT-PCR using gene-specific primers. This makes it possible to validate the specific mRNA transcripts that are binding targets of that miRNA. It is also possible to profile the immunoprecipitated mRNA molecules using conventional expression microarrays or RNA-Seq.
American Research Products Inc Rat p53 tumor protein p53 TP5
Rat p53/tumor protein p53/TP53 ELISA Kit from CUSABIO Detection Range 12 5 pg/mL-800 pg/mL Sensitivity 3 12 pg/mL Sample serum plasma tissue homogenates Method Sandwich
Abcam BVT 948, Protein tyrosine phosphatase (PTP) inhibitor, 50MG
MW 241.24 Da, Purity >99%. Non-competitive, irreversible protein tyrosine phosphatase (PTP) inhibitor (IC₅₀ values are 0.9 (PTP1B), 1.7 (TCPTP), 0.09 (SHP-2), 1.5 (LAR) and 0.7 (YopH) μM). Active in vivo and cell-permeable.
The product is subject to the following: Abcam Restricted Use Statement
Zymo Research Corporation Methylated-DNA IP Kit, 10 Rxns.
The Methylated-DNA IP Kit enriches 5-methylcytosine (5-mC) containing DNA from any pool of fragmented genomic DNA for use in genome-wide methylation analysis.The kit includes a highly specific anti-5-methylcytosine monoclonal antibody for the “capture” and separation of methylated DNA from non-methylated DNA in only a fewhours. Typically, over a hundred-fold enrichment of methylated DNA vs. non-methylated DNA can be achieved with the use of this kit. Recovered DNA is suitable for many downstream applications to analyze genome-wide DNA methylation including: PCR, qPCR, whole-genome amplification, MeDIP-Seq, etc.
Cayman Chemical MK2 InhIbItr III 1mg
A potent, cell-permeable inhibitor of MK2 (IC50 = 8.5 nM); less potently blocks MK3 and MK5 (IC50s = 210 and 81 nM, respectively) and is weak or inactive against several other kinases, including other p38 MAP kinase targets; prevents LPS-induced synthesis of TNF-α in human monocyte-like U937 cells (IC50 = 4.4 µM)
Abcam Immunoprecipitation kit, 25 Tests
Abcam's Immunoprecipitation Kit (ab206996) can be used to perform immunoprecipitation (IP) and Co-IP for functional studies of immunoprecipitated proteins/complexes and SDS-PAGE or western blot analysis of immunoprecipitated proteins and complexes.
The product is subject to the following: Abcam Restricted Use Statement
ABclonal Technology MAPKAPK-2/MK2 Rabbit pAb
This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.
Active Motif ChIP-IT Express Enzymatic Shearing Kit (included in 53009)
The first step in successful chromatin immunoprecipitation is shearing the chromatin into 200-1000 bp fragments. This has traditionally been performed by subjecting the isolated chromatin to different pulses of sonication. Sonication is an effective method for shearing DNA, and is the most widely-used method. In some circumstances, however, it can be difficult to optimize due to complications arising from emulsification and overheating. Because of this, Active Motif has developed a robust and user-friendly method to shear chromatin by enzymatic treatment. The Enzymatic Shearing Kit uses a proprietary Enzymatic Shearing Cocktail that quickly shears DNA into 200-1000 bp fragments. Because enzymatic shearing is solely time and temperature dependent, the problems associated with sonication are eliminated and ChIP results are improved.
LGC GENOMICS LLC (Lucigen) MPC Protein Precipitation Solution, 500 mLA simple solution and method for the removal of proteins from cell lysates prior to nucleic acid purification. To be used with the MasterPure Kits
This reagent is designed for use with the MasterPure™ kit protocols only.
Cayman Chemical MK2 InhIbItr IV 10mg
A highly selective, non-ATP competitive inhibitor of p38/mitogen-activated protein kinase-activated protein kinase 2 (IC50 = 0.11 μM); inhibits TNF-α (IC50 = 4.4 μM) and IL-6 (IC50 = 5.2 μM) secretion from the human THP-1 acute monocytic leukemia cell line
ABclonal Technology PARP1 Rabbit pAb
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
Cayman Chemical Methylated LysIn PolycLNal
Immunogen: Methylated KLH • Host: Rabbit • Species reactivity: (+) Species independent • Applications: ELISA, ICC, IF, IP, WB • Lysine can be methylated once, twice, or three times by lysine methyltransferases. The transfer of methyl groups from SAM to histones is catalyzed by histone methyltransferases.
Cayman Chemical NavenIFlex Cel MR RED 100RXN
A DUSP26 inhibitor (IC50 11.62 UM) induces IMR-32 neuroblastoma cell apoptosis (IC50 4.13 UM)
Abcam Mycophenolate mofetil, MPDH inhibitor, 250MG
MW 433.5 Da, Purity >98%. Selective, non-competitive and reversible IMPDH inhibitor (IC₅₀ = 3 μM). Immunosuppressant agent. Inhibits guanosine synthesis. Shows antiparasitic effects and suppresses T cells and B cells in vivo. Orally active.
The product is subject to the following: Abcam Restricted Use Statement
Abcam Cyclic Pifithrin-alpha hydrobromide, pifithrin-alpha analog, 50MG
MW 349.3 Da, Purity >98%. Stable, cell-permeable analog of pifithrin-α (ab120478). Inhibits p53-mediated apoptosis and p53-dependent gene transcription. Prevents dexamethasone-induced cell death in murine thymocytes (EC₅₀ = 2.01 μM). Potent STAT6 transcriptional inhibitor. Active in vivo and in vitro. See similar compound
The product is subject to the following: Abcam Restricted Use Statement