Antibody Panels and Kits
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MilliporeSigma™ Upstate ChIPAb+ Trimethyl-Histone H4 (Lys20) - ChIP Validated Antibody and Primer Set
Trimethyl histone H4 (Lys20) antibody against synthetic peptide corresponding to amino acids 18-22 of histone H4 trimethylated on lysine 20
MilliporeSigma™ Upstate™ p53α, Mouse monoclonal, ChIP Validated Antibody and Primer Set
Mouse Monoclonal Antibody
| Content And Storage | −20°C for one year from date of shipment |
|---|---|
| Host Species | Mouse |
| Applications | ChIP Assay,Western Blot |
| Form | Purified |
| Gene Accession No. | P04637 |
| Isotype | IgG |
| Includes | This ChIPAb+ p53 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers. |
| Antigen | p53α |
| Regulatory Status | RUO |
| Gene Symbols | P53, TP53 |
| Purification Method | Protein A purified |
| Gene ID (Entrez) | NP_000537 |
| Formulation | Anti-p53 (mouse monoclonal IgG). One vial containing 25μg of of protein A purified mouse IgG2a in 25μL of storage buffer (PBS, pH 7.4, containing 0.05% sodium azide and 1mg/mL BSA). Normal Mouse IgG. One vial containing 25μg of normal mouse IgG in 25μL volume. ChIP Primers p21. One vial containing 75μL of 5μM of each primer specific for a region of the human p21 (WAF1/CIP1/CDKN1A) promoter. FOR: GTG GCT CTG ATT GGC TTT CTG; REV: CTG AAA ACA GGC AGC CCA AG |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Test Specificity | Recognizes p53 in wild type and mutant forms of human origin. |
MilliporeSigma™ Upstate ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set
Dimethyl-histone H3 (Lys9) purified antibody is made against synthetic peptide (dimethylated at Lys9) corresponding to amino acids 1-18 of histone H3
| Content And Storage | -20°C for one year from date of shipment; Upon first thaw and prior to removing the cap centrifuge the vial and gently mix the solution; Aliquot into microcentrifuge tubes and store at −20°C; Avoid repeated freeze/thaw cycles |
|---|---|
| Host Species | Mouse |
| Applications | ChIP Assay,Western Blot |
| Form | Purified |
| Gene Accession No. | Q16695 |
| Isotype | IgG2b κ |
| Includes | This ChIPAb+ Monomethyl-Histone H3 (Lys4) -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers. |
| Antigen | Monomethyl-Histone H3 (Lys4)α |
| Regulatory Status | RUO |
| Gene Symbols | HIST3H3; H3FT; H3/t; H3t; MGC126888; H3/g; H3.4; H3T; MGC126886 |
| Purification Method | Protein A purified |
| Gene ID (Entrez) | NM_003493 |
| Formulation | Anti-Monomethyl-HisPtone H3 (Lys4) (mouse monoclonal), One vial containing 50μg of protein G purified antibody in 50μL PBS containing 0.05% sodium azide. Normal Mouse IgG. Two vials containing 25μg purified Mouse IgG in 25μL storage buffer containing 0.1% sodium azide. ChIP Primers, human GAPDH coding region, . One vial containing 75μL of 5μM each primer specific for human GAPDH coding region. FOR: GGC TCC CAC CTT TCT CAT CC; REV: GGC CAT CCA CAG TCT TCT GG |
| Immunogen | Peptide corresponding to Histone H3 containing the sequence [ART(me1-K)QTARKSTGC] on which Lys4 is acetylated. |
| Classification | Monoclonal |
| Primary or Secondary | Primary |
| Test Specificity | This antibody detects Monomethyl-Histone H3 methylated at Lys4. |
| Content And Storage | −20°C for one year |
|---|---|
| Host Species | Rabbit |
| Applications | ChIP Assay,Western Blot |
| Form | Purified |
| Gene Accession No. | Q16695 |
| Includes | Trimethyl-Histone H3 (Lys27) ChIP validated Antibody and Primer set including the ChIP-grade antibody and the specific control PCR primers used for chromatin immunoprecipitation of H3K27Me3. |
| Antigen | Trimethyl-Histone H3 (Lys27) |
| Regulatory Status | RUO |
| Gene Symbols | H3F3A, MGC87783, H3.3A, MGC87782, H3F3, H3.3B, H3F3B |
| Gene ID (Entrez) | NP_003484 |
| Formulation | Anti-trimethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100μg protein A purified IgG in 100μL buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide. Normal Rabbit IgG. One vial containing 125μg purified Rabbit IgG in 125μL storage buffer containing 0.1% sodium azide. ChIP Primers human alpha-Satellite. One vial containing 75μL of 5μM of each control primer specific for human alpha-Satellite. FOR: CTG CAC TAC CTG AAG AGG AC; REV: GAT GGT TCA ACA CTC TTA CA |
| Immunogen | KLH-conjugated,synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human Histone H3. |
| Classification | Polyclonal |
| Primary or Secondary | Primary |
| Test Specificity | trimethyl-Histone H3 (Lys27) |
MilliporeSigma™ Upstate ChIPAb+ REST - ChIP Validated Antibody and Primer Set
REST purified antibody against GST fusion protein corresponding to amino acids 801-1097 of human REST
MilliporeSigma™ RIPAb+™ PABPC1 RIP Validated Antibody and Primer Set
This RIPAb+ PABPC1 -RIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers.
MilliporeSigma™ Upstate™ Acetyl-Histone H4, Rabbbit Polyclonal, ChIP Validated Antibody and Primer Set
Rabbit Polyclonal Antibody
| Content And Storage | −20°C from date of receipt |
|---|---|
| Host Species | Rabbit |
| Applications | Multiplex,Functional Assay,Immunoassay,Western Blot |
| Form | Serum |
| Gene Accession No. | P62805 |
| Includes | This ChIPAb+ Acetyl-Histone H4 -ChIP Validated Antibody and Primer Set conveniently includes the antibody and the specific control PCR primers. |
| Antigen | Acetyl-Histone H4 |
| Regulatory Status | RUO |
| Gene ID (Entrez) | NP_778224 |
| Formulation | Anti-Acetyl-Histone H4 (rabbit polyclonal IgG). One vial containing 100μL of antiserum containing 0.05% sodium azide. Normal Rabbit Serum. One vial containing 100μL antiserum containing 0.05% sodium azide. Control Primers. One vial containing 75μL of 5μM of each primer specific for for human GAPDH. |
| Immunogen | The acetyl-histone H4 antiserum is made against a peptide corresponding to amino acids 2-19 of Tetrahymena histone H4. |
| Classification | Polyclonal |
| Primary or Secondary | Primary |
| Test Specificity | Recognizes acetylated histone H4 of approximately 10kDa. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly crossreacts with acetylated histone H2B from HeLa cells. May cross-react with other acetylated proteins. |
| Content And Storage | Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | ELISA,Western Blot,Immunocytochemistry,Immunohistochemistry (Paraffin),Flow Cytometry,Immunofluorescence |
| Form | Liquid |
| Isotype | IgG1 |
| Concentration | Antibody 1 mg/mL |
| Antigen | PD1 |
| Regulatory Status | RUO |
| Purification Method | Antibodies are supplied as protein A purified IgG1. |
| Product Type | Detection Sets |
| Formulation | PBS containing 0.02% sodium azide. |
| Classification | Monoclonal |
| Immunogen | Mouse monoclonal PD-1 antibodies were raised against the extracellular domain of human PD-1. |
| Primary or Secondary | Primary |
| Content And Storage | Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | ELISA,Western Blot,Immunocytochemistry,Immunohistochemistry (Paraffin),Flow Cytometry,Immunofluorescence |
| Form | Liquid |
| Isotype | IgG1,IgG2b |
| Concentration | Antibody 1 mg/mL |
| Antigen | CD80 |
| Regulatory Status | RUO |
| Purification Method | Antibodies are supplied as protein A purified IgG1 and IgG2b. |
| Product Type | Detection Sets |
| Formulation | PBS containing 0.02% sodium azide. |
| Classification | Monoclonal |
| Immunogen | CD80 antibodies were raised against the extracellular domain of human CD80. |
| Primary or Secondary | Primary |
| Content And Storage | LAG3 antibodies should be stored in working aliquots at -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
|---|---|
| Target Species | Human |
| Host Species | Llama |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunohistochemistry (Paraffin),Immunofluorescence,Flow Cytometry |
| Form | Liquid |
| Isotype | Not Applicable |
| Concentration | Antibody 1 mg/mL |
| Antigen | LAG3 |
| Regulatory Status | RUO |
| Purification Method | LAG3 Antibodies are affinity chromatography purified via Nickel column. Antibodies are supplied as a His-tagged purified protein. They also contains a myc-tag for detection. |
| Product Type | Detection Sets |
| Formulation | LAG3 Antibodies are supplied in PBS. |
| Classification | Monoclonal |
| Immunogen | LAG3 antibodies were raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3. |
| Primary or Secondary | Primary |
| Content And Storage | Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | ELISA,Western Blot,Immunocytochemistry,Immunohistochemistry (Paraffin),Flow Cytometry,Immunofluorescence |
| Form | Liquid |
| Isotype | IgG1,IgG2a |
| Concentration | Antibody 1 mg/mL |
| Antigen | LAG3 |
| Regulatory Status | RUO |
| Purification Method | Antibodies are supplied as protein A purified IgG1 and IgG2a. |
| Product Type | Detection Sets |
| Formulation | PBS containing 0.02% sodium azide. |
| Classification | Monoclonal |
| Immunogen | LAG3 antibodies were raised against the extracellular domain of human LAG3. |
| Primary or Secondary | Primary |
| Content And Storage | Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | ELISA,Western Blot,Immunocytochemistry,Immunohistochemistry (Paraffin),Flow Cytometry,Immunofluorescence |
| Form | Liquid |
| Isotype | IgG1 |
| Concentration | Antibody 1 mg/mL |
| Antigen | PDL1 |
| Regulatory Status | RUO |
| Purification Method | Antibodies are supplied as protein A purified IgG1. |
| Product Type | Detection Sets |
| Formulation | PBS containing 0.02% sodium azide. |
| Classification | Monoclonal |
| Immunogen | PD-L1 antibodies were raised against the extracellular domain of human PD-L1. |
| Primary or Secondary | Primary |
| Content And Storage | Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | ELISA,Western Blot,Immunocytochemistry,Immunohistochemistry (Paraffin),Flow Cytometry,Immunofluorescence |
| Form | Liquid |
| Isotype | IgG1 |
| Concentration | Antibody 1 mg/mL |
| Antigen | TMIGD2 |
| Regulatory Status | RUO |
| Purification Method | Antibodies are supplied as protein A purified IgG1. |
| Product Type | Detection Sets |
| Formulation | PBS containing 0.02% sodium azide. |
| Classification | Monoclonal |
| Immunogen | TMIGD2 |
| Primary or Secondary | Primary |
| Content And Storage | Antibodies can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
|---|---|
| Target Species | Human |
| Host Species | Mouse |
| Conjugate | Unconjugated |
| Applications | ELISA,Western Blot,Immunocytochemistry,Immunohistochemistry (Paraffin),Flow Cytometry,Immunofluorescence |
| Form | Liquid |
| Isotype | IgG1 |
| Concentration | Antibody 1 mg/mL |
| Antigen | CTLA4 |
| Regulatory Status | RUO |
| Purification Method | Antibodies are supplied as protein A purified IgG1. |
| Product Type | Detection Sets |
| Formulation | PBS containing 0.02% sodium azide. |
| Classification | Monoclonal |
| Immunogen | Mouse monoclonal CTLA-4 antibodies were raised against the extracellular domain of human CTLA-4. |
| Primary or Secondary | Primary |