Restriction Enzymes
New England Biolabs, Inc. Esp3I – 1500 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN_....
Cayman Chemical BAM-22P8-22humnmouse10mg
A neuropeptide and MRGPRX1 agonist; induces calcium mobilization in HEK293S cells expressing human MRGPRX1 or SNSR3 (EC50s = 14 and 28 nM, respectively); inhibits voltage-induced calcium currents in isolated rat neurons expressing human MRGPRX1 (EC50 = 0.6 µM); intrathecal administration decreases the tail-flick latency in pertussis toxin-stimulated rats and increases the tail-flick latency in U-73122-stimulated rats, indicating changes in pain sensitivity, at 30 nmol/animal; increases scratching behavior in wild-type mice and enhances bile-duct ligation-induced scratching behavior in a mouse model of cholestasis
New England Biolabs, Inc. NheI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence G^CTAGC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. XRN-1 – 100 units
XRN-1 is highly processive 53 exoribonuclease, requiring 5 monophosphate. It also acts on 5' monophosphate ssDNA with greatly reduced efficiency.
ABclonal Technology SacII
5...C C G C/ G G...3 3...G G/ C G C C...5 Isoschizomers: Cfr42I KspI Sfr303I SgrBI
New England Biolabs, Inc. FatI – 50 units
A restriction endonuclease that recognizes the sequence ^CATG_.
MilliporeSigma GSK-3BETA RECOM ENZYME
GSK3 beta active Serine/Threonine Phosphorylation 5mcg 125mcg 250mcg Protein Phosphorylation Assay: 5-40ng PURITY:>95% by SDS-PAGE STABILITY: 6 months at - 20degreeC PROPERTIES: Specific activity guaranteed between 932 and 1269U/mg