Restriction Enzymes
New England Biolabs, Inc. PluTI – 500 units
A restriction endonuclease that recognizes the sequence GGCGC^C
New England Biolabs, Inc. KpnI-HF – 20000 units
A high fidelity restriction endonuclease that recognizes the sequence G_GTAC^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. XRN-1 – 100 units
XRN-1 is highly processive 53 exoribonuclease, requiring 5 monophosphate. It also acts on 5' monophosphate ssDNA with greatly reduced efficiency.
New England Biolabs, Inc. SpeI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence A^CTAGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
INTACT GENOMICS INC PEmax Enzyme 80 µg
Intact Genomics (IG) PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-Murine Moloney Leukemia Virus-Reverse Transcriptase (MMLV-RT) fusion protein3-4 containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR prime-editing3-4. The physical purity of this enzyme is ≥85% as assessed by SDS-PAGE with Coomassie blue staining.Product Includes: PEmax enzyme 5x PEmax Nicking Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA, pH 6.5 @ 25 °CStore all components at -20°C.
New England Biolabs, Inc. BstZ17I-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence GTA^TAC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BsrFI-v2 – 1000 units
A restriction endonuclease that recognizes the sequence R^CCGG_Y.
New England Biolabs, Inc. BbsI HF - 1000 units
A high fidelity restriction endonuclease that recognizes the sequence GAAGAC(N)2. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind HF restriction enzymes are fully active under a broader range of conditions minimizing off-target products while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BsmBI-v2 – 1000 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN and is optimized for Golden Gate Assembly.
- Time-Saver qualified for digestion in 5-15 minutes
- Used in Golden Gate Assembly
- Type IIS Res recognize asymmetric DNA sequences; cleave outside of their recognition site
- Supplied with 1 vial of Gel Loading Dye, Purple (6X)
New England Biolabs, Inc. Nb.BsrDI – 1000 units
A restriction endonuclease that recognizes the sequence GCAATG_NN.
New England Biolabs, Inc. T7 Endonuclease I – 1250 units
T7 Endonuclease I recognizes and cleaves non-perfectly matched DNA, cruciform DNA structures, Holliday structures or junctions, heteroduplex DNA and more slowly, nicked double-stranded DNA. The cleavage site is at the first, second or third phosphodiester bond that is 5' to the mismatch. The protein is the product of T7 gene 3.
New England Biolabs, Inc. ScaI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence AGT^ACT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
New England Biolabs, Inc. BsaI-HF®v2 – 5000 units
BsaI-HFv2 is a Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNA cutting by BsaI. Works well for any protocol requiring DNA cutting by BsaI. Recommended enzyme for any purpose requiring digestion at the recognition sequence ...5-GGTCTC(N1)/(N5)-3...
- Engineered for improved performance
- Used in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer
- Type IIS REs recognize asymmetric DNA sequences; cleave outside their recognition sequence
- 100% activity in rCutSmart Buffer
- Time-Saver qualified for digestion in 5-15 minutes