Substrates
Apexbio Technology LLC D-Luciferin(Synonyms: Firefly Luciferin, Luciferin, Beetle Luciferin, (S)-2-(6'-Hydroxybenzothiazolyl)-Δ2-thiazoline-4-carboxylic acid), 200mg, CAS: 2591-17-5.
D-Luciferin (CAS 2591-17-5) is a membrane-permeable bioluminescent substrate for firefly luciferase characterized by a Km of approximately 2 M In the presence of ATP luciferin undergoes oxidation and decarboxylation catalyzed by luciferase emitting measurable photons This bioluminescence enables quantification of intracellular ATP levels both in vitro and in vivo In biomedical research D-Luciferin is commonly applied for monitoring promoter-driven luciferase gene expression and as a non-invasive imaging probe in bioluminescence imaging (BLI) studies to assess tumor burden pharmacodynamics and cellular ATP content
Cayman Chemical PKC Pseudosubstrate InhIbIto
A synthetic peptide that corresponds to amino acid residues of the pseudosubstrate domain of PKCθ
Sigma Aldrich Fine Chemicals Biosciences 2-Nitrophenyl Beta-D-Glucopyranoside | 2816-24-2 | MFCD00065048 | 250mg
2-Nitrophenyl Beta-D-Glucopyranoside | Mol Wt: 301.25 | 2816-24-2 | MFCD00065048 | 250mg
VAZYME INTERNATIONAL LLC Add&Read Phospho-ERK1/2 (Thr202/Tyr204) Kit, 500 tests
This kit is designed for the detection of phosphorylated ERK protein content in cells. It utilizes the Add&Read TR-FRET no-wash ELISA methodology, offering a streamlined two-step workflow that significantly saves time and labor compared to traditional ELISA methods.
- Based on TR-FRET methodology (Time-Resolved Fluorescence Resonance Energy Transfer).
- Employs a two-step workflow.
- Saves over 80 percent of time and labor compared to traditional ELISA methods.
- Utilizes a no-wash ELISA assay type.
- Designed for the detection of phosphorylated ERK protein content in cells.
- Provided in a 500-test size.
Medchemexpress LLC Boc-LRR-AMC | 700.83 g/mol | C33H52N10O7 | 10MG
Boc-LRR-AMC (Boc-Leu-Arg-Arg-AMC) is a fluorogenic substrate which can be utilized for detecting the trypsin-like activity of the 26S proteasome or the 20S proteasome core[1]
Medchemexpress LLC Boc-val-pro-arg-AMC hydrochloride | 70375-24-5 | MFCD00058181 | 99.4% | 664.19 | C31H46ClN7O7 | 5 MG
Boc-Val-Pro-Arg-AMC hydrochloride is a sensitive fluorogenic peptide substrate used to measure trypsin-like serine protease activity. Proteolytic cleavage releases the fluorescent AMC (7-amino-4-methylcoumarin) leaving group, enabling both continuous and end-point fluorometric assays. Intended for research use only.
Echelon Biosciences Research Labs BIOTIN LABELED PI(4,5)P2 10ug
Biotin Phosphatidylinositol 4,5-bisphosphate is a water soluble analog of PI(4,5)P2 (PIP2) labeled with biotin at the sn-1 position.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 4,5-bisphosphate (PIP2) has been shown to play a central role in a variety of cellular functions. Amongst its many functions, PIP2 is a substrate for Phospholipase C-coupled G-protein pathways involved in intracellular calcium release in a number of tissues. It is also a substrate for class I phosphoinositide 3-kinase (PI3-K) forming PI(3,4,5)P3. This item is non returnable
Advansta Inc WesternBright Sirius Chemiluminescent Detection Kit 200ml
WesternBright Sirius is the most sensitive HRP substrate available from Advansta for chemiluminescent Western blotting. With attomole sensitivity and a long-lasting signal, WesternBright Sirius allows you to detect bands not visualized with other substrates. High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands.
![Research Products International Corp MUG [4-Methylumbelliferyl-B-D-glucuronide Trihydrate], 100 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M65900-0.1.JPG-250.jpg)
Research Products International Corp MUG [4-Methylumbelliferyl-B-D-glucuronide Trihydrate], 100 Milligrams
Fluorescent substrate for β-D-Glucuronidase (GUS) encoded by the gusA gene isolated from E. coli. Fluorescence assay allows quantitation of GUS activity in protein extracts at peak excitation of 365 nm (UV) and a peak emission of 455 nm (blue).
Cayman Chemical D-LucIferIn 100mg
A chemiluminescent substrate of firefly luciferase which produces light upon oxidative decarboxylation in the presence of ATP
Enzo Life Sciences OMNIMMP RED fluorogenic substrate (100µg)
Highly-quenched, ultra-bright fluorogenic substrate for most MMPs. 6’-TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties. The OMNIMMP RED substrate offers key advantages over other MMP substrates. Purity: ≥90% (HPLC). Solubility: Soluble in DMSO (50mM). Long Term Storage: -20°C.
Enzo Life Sciences Ac-IETD-AFC (5 mg)
Caspase and granzyme substrate. Fluorogenic substrate for caspase-8/caspase-3 processing enzyme and granzyme B. Also a substrate for caspase-10. The IETD sequence is based on caspase-3 proenzyme cleavage site. Alternative Name: Caspase 8 substrate (fluorogenic), Granzyme B substrate (fluorogenic). Formula: C31H38F3N5O12. MW: 729.7. Purity: ≥98% (HPLC). Sequence: Ac-Ile-Glu-Thr-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin). Appearance: White to off-white powder. Solubility: Soluble in DMSO. Long Term Storage: -20°C. Handling: Keep cool and dry.
Sino Biological Peptide Substrates: Chktide
The Chktide peptide sequence (KKKVSRSGLYRSPSMPENLNRPR) is based on the human CDC25C protein isoform A (amino acid 205-225).
Medchemexpress LLC D-luciferin potassium | 115144-35-9 | MFCD00044928 | 99.97% | 318.41 | C11H7KN2O3S2 | 100MG
D-luciferin potassium is the water-soluble potassium salt of D-luciferin, the natural substrate for firefly luciferase. It is provided as a solid for preparation of aqueous solutions used in both in vitro and in vivo bioluminescent assays and imaging applications.
- Water-soluble potassium salt of D-luciferin.
- Serves as substrate for firefly luciferase in bioluminescent assays.
- High purity suitable for research applications (reported 99.97%).
- Applicable to both in vitro assays and in vivo imaging workflows.
- Soluble in aqueous buffers for easy preparation of working solutions.
- Molecular weight 318.41; chemical formula C11H7KN2O3S2.