Substrates
Cayman Chemical DocosahexaenoIc ACD CYP450 O
A mixture that contains (±)7(8)-EpDPA, (±)10(11)-EpDPA, (±)13(14)-EpDPA, (±)16(17)-EpDPA, (±)19(20)-EpDPA, (±)7(8)-DiHDPA, (±)10(11)-DiHDPA, (±)13(14)-DiHDPA, (±)16(17)-DiHDPA, and (±)19(20)-DiHDPA
Sino Biological Peptide Substrates: Modified AKT Substrate
The Modified AKT Substrate peptide sequence (modified-CKRPRAASFAE) is based on the N-terminus of GSK3.
Sino Biological Lipid Substrates: PI(4,5)P2:PS
The substrate solution used for assaying lipid kinases.
Enzo Life Sciences Ac-WEHD-AMC (5mg)
Fluorogenic substrate for caspase-1 (Km=4µM), -4 (Km=31µM);and -5 (Km=15µM). This fluorogenic caspase-1 substrate with an optimal tetrapeptide sequence is cleaved 50-fold more efficiently than Ac-YVAD-AMC (ALX-260-024). Alternative name: Caspase 1 substrate (fluorogenic), Caspase 5 substrate (fluorogenic), Caspase 4 substrate (fluorogenic). Formula: C38H40N8O11. MW: 784.8. Purity: ≥98% (HPLC). Formulation: Lyophilized. Solubility: Soluble in DMSO (100mM, 75.5mg/ml). Dilute with distilled water or buffer. Long Term Storage: -20°C.
Neogen Diagnostics K-Gold PNPP Substrate, 500 mL, Microwell, AP, Colorimetric
K-Gold Substrate is a one-bottle stabilized chromogenic substrate for use with alkaline phosphatase based immunoassays. K-Gold Substrate utilizes the substrate p-nitrophenyl-phosphate (PNPP), which reacts with alkaline phosphatase to produce the chromophore p-nitrophenol. NEOGENs ready-to-use K-Gold formula does not require mixing of additional ingredients or stabilizing agents, making it an ideal formula for kit manufacturers. Stable for a minimum of 30-months when stored at 2-8C.
Medchemexpress LLC Ac-Trp-Leu-Ala-AMC | 1104011-59-7 | 99.8% | 587.7 g/mol | C32H37N5O6 | 10MG
Ac-WLA-AMC (Ac-Trp-Leu-Ala-AMC; CAS 1104011-59-7) is an acetylated tripeptidyl fluorogenic substrate for measuring the chymotrypsin-like activity of the 20S proteasome β5 (β5c) subunit. Enzymatic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal used in proteasome activity assays and inhibitor screening.
- Measures chymotrypsin-like proteasome β5 activity.
- Releases 7-amino-4-methylcoumarin (AMC) for sensitive fluorescence detection.
- Compatible with kinetic and end-point fluorescence assays.
- Supplied as a solid peptide substrate suitable for biochemical assays.
- High purity (99.8%) appropriate for research use.
Abcam Carbo x yl-DCFDA (5(6)-Carbo x y-2',7'-dichlorofluorescein diacetate), Fluorogenic esterase substrate, 1G
MW 529.28 Da, Purity >85%. Amine-reactive, fluorogenic, non-selective esterase substrate. Hydrolyses to DFCA (ab145439) fluorophore intracellularly. Labels intact cells. 2,7-Dichlorofluorescein (ab146332) derivative and DCFDA N-succinimidyl ester (ab145286) precursor.
The product is subject to the following: Abcam Restricted Use Statement
AnaSpec 520 MMP FRET Substrate I, Sensitive FRET Assay Tool, High Purity ≥95%, 0.1 mg
The 520 MMP FRET Substrate I is a sensitive fluorogenic peptide specially designed for detecting Matrix Metalloproteinases (MMPs). Its unique FRET mechanism allows rapid enzymatic activity measurements, particularly for MMP-13. This substrate excels in research areas like cancer and apoptosis, offering a reliable way to quantify protease activity with clear fluorescent readouts, differentiate enzymatic activity, and ensure reliable results for scientific studies.
Enzo Life Sciences OMNICATHEPSIN fluorogenic substrate (10mg). CAS: 65147-22-0
Fluorogenic substrate cleaved by plasma kallikrein and kallikrein 8, but not plasmin; by papain; and by most cathepsins (cathepsins B, C/DPP-I, F, K/O2, L, L2/V, O, S, and X/Z). Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Alternative name: Z-FR-AMC, Z-Phe-Arg-AMC. Purity: ≥99%. Long Term Storage: -20°C.
Abcam Carbo x yumbelliferyl ß-D-glucuronide (CUGlcU), 10MG
MW 382.27 Da, Purity >95%. Water soluble fluorogenic GUS substrate similar to MUGlcU. Useful for measuring cloned β-glucuronidase activity in plant lysate, homogenate, leaf disk or whole plant assays.
The product is subject to the following: Abcam Restricted Use Statement
Abcam 5-CFDA (5-Carbo x yfluorescein Diacetate), Fluorogenic non-selective esterase substrate, 25MG
MW 460.4 Da, Purity >95%. Hydrophobic, cell-permeable fluorogenic non-selective esterase substrate. Becomes hydrolyzed to hydrophilic strongly fluorescent 5-Carboxyfluorescein (ab145293) intracellularly. Measures intracellular pH. 5-CFDA N-succinimidyl ester (ab145292) precursor.
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Her2/CD340 protein, canine (HEK293, His) | >95.0% | 100 UG
HER2/CD340 Protein, Canine (HEK293, His) is a recombinant, partially truncated canine ErbB2 extracellular domain produced in HEK293 mammalian cells and supplied as a C-terminal His-tagged, lyophilized protein for research applications. It is suitable for biochemical and cell-based assays, including binding and functional activity measurements.
- Recombinant partial extracellular domain (T23-T652) expressed in HEK293 cells.
- C-terminal His tag for purification and detection.
- Supplied lyophilized for extended stability.
- Purity greater than 95% as determined by reducing SDS-PAGE.
- Low endotoxin level (<1 EU/μg), suitable for sensitive assays.
- Store at -20°C; after reconstitution stable 4°C for 1 week or -20°C for long-term storage.
- Demonstrated biological activity with ED50 0.4594 μg/mL in the presence of 0.6 μg/mL trastuzumab.
Enzo Life Sciences FLUOR DE LYS-Green substrate (50µl)
The FLUOR DE LYS-Green substrate offers a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has two steps. First, the FLUOR DE LYS-Green Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS Developer (BML-KI105) produces a fluorophore. The FLUOR DE LYS-Green substrate has a higher wavelength excitation and emission than the original FLUOR DE LYS substrate (BML-KI104). This avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue range. Formulation: 50mM solution in DMSO. Purity: ≥95% (HPLC). Long Term Storage: -80°C.
Medchemexpress LLC D-luciferin potassium | 115144-35-9 | 100.0% | 318.41 | C11H7KN2O3S2 | 250MG
D-Luciferin potassium is the potassium salt of D-luciferin, the natural substrate for firefly luciferase. In the presence of ATP and appropriate cofactors it undergoes enzymatic oxidation to produce rapid, proportional chemiluminescent light, making it suitable for reporter gene assays, cell viability measurements, and sensitive ATP detection. Supplied as a high-purity solid and available in multiple pack sizes for research use.
- High-purity potassium salt for reliable luminescent assays.
- Produces rapid, proportional chemiluminescent signal.
- Compatible with luciferase-based reporter and ATP assays.
- Supplied as a solid, stable form for storage and handling.
- Available in a range of pack sizes to suit experimental needs.
Medchemexpress LLC HER2/CD340 Human B 100ug | 100ug
The HER2/CD340 protein is a dynamic tyrosine kinase that is essential in the neuregulin receptor complex and regulates microtubule dynamics Upon activation it triggers the MEMO1-RHOA-DIAPH1 pathway inhibits GSK3B and promotes the association of APC and CLASP2 on the cell membrane to achieve microtubule stabilization HER2/CD340 Protein Human (Biotinylated Domain 4 HEK293 His-Avi) is the recombinant human-derived HER2/CD340 protein expressed by HEK293 with C-His-Avi labeled tag