Substrates
Cayman Chemical Ac-DNLD-AMC 5mg
A fluorogenic caspase-3 substrate; upon enzymatic cleavage by caspase-3, AMC is released and its fluorescence can be used to quantify caspase-3 activity; AMC ex/em = 340-360/440-460 nm, respectively
Medchemexpress LLC Gly-Pro-AMC hydrobromide | 115035-46-6 | 98.68% | 410.26 | 50 MG
Gly-Pro-AMC hydrobromide is a fluorescent dye that can be used as a specific fluorescent substrate for detecting Dipeptidyl peptidase IV (DPP-IV) activity. It exhibits fluorescence properties with an excitation wavelength of 350 nm and fluorescence emission spectrum detection at 450 nm.
- Specific fluorescent substrate for detecting DPP-IV activity
- Fluorescence properties with excitation wavelength of 350 nm
- Fluorescence emission spectrum detection at 450 nm
- Can be used at 50 μM for 20 min at 37 °C
Medchemexpress LLC D-Luciferin 6 -methy 10mM 1mL | 24404-90-8 | 294.35 g/mol | C12H10N2O3S2 | 1 ML
D-Luciferin 6'-methyl ether is a synthetic luciferin derivative used as a firefly luciferase inhibitor and biochemical reagent for bioluminescence assays. It is supplied as a solid or as a pre-made 10 mM solution in DMSO for convenient use in inhibitor studies and assay development.
- Available as solid (multiple mg sizes) or 10 mM solution in DMSO, 1 mL.
- High purity (99.55%) suitable for biochemical assays.
- Molecular weight 294.35 g/mol; formula C12H10N2O3S2.
- Used to study luciferase activity and inhibition in bioluminescent assays.
- Soluble in DMSO; follow supplier storage recommendations.
CPC Scientific Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-psi-(COO)Ala-Ser-Lys(DABCYL)-NH2 (trifluoroacetate salt) 1MG
A HCV protease substrate that incoporates an ester bond between residues P1 and P1. Due to ready transesterification of the scissile bond to the acyl-enzyme intermediate, this substrate shows very high kcat/Km values, enabling detection of activity with subnanomolar nonstructural protein 3 (NS3 protease) concentrations. It is widely used for the continuous assay of NS3 protease activity. Substrate cleavage is proportional to the enzyme concentration with a detection limit for NS3 between 1 nM and 250 pM. Upon cleavage of this substrate, fluorescence can be monitored at Abs/Em = 355/500 nm. As an internally quenced fluorogenic substrate, it can be used to monitor HCV NS3 protease activity.SEQUENCE: Ac-Asp-Glu-Asp(EDANS)-Glu-Glu-Abu-psi-(COO)Ala-Ser-Lys(DABCYL)-NH2 (trifluoroacetate salt)ONE-LETTER SEQUENCE: Ac-DED(EDANS)-EE-Abu-psi-(COO)AS-K(DABCYL)-NH2MOLECULAR FORMULA: C68H89N15O25S1MOLECULAR WEIGHT: 1548.6STORAGE CONDITIONS: -20 5 CCAS REGISTRY NUMBER: SYNONYMS: Ac-
Medchemexpress LLC Ac-lys-AMC (Hexanamide) | 156661-42-6 | 98.9% | 1 ML
Ac-Lys-AMC (Hexanamide), also known as MAL, is a fluorescent substrate utilized for assaying histone deacetylases (HDACs). It is intended strictly for research purposes and is not sold to patients.
- Fluorescent substrate.
- Designed for the assay of histone deacetylase HDACs.
- Purity of 98.93%.
- Appears as a white to off-white solid.
- Soluble in DMSO at 100 mg/mL in vitro, requiring sonication.
- In vivo protocols offer clear solutions with solubility of ≥ 2.5 mg/mL in various co-solvent systems.
- Powder stable for 3 years at -20°C and 2 years at 4°C.
- In solvent, stable for 6 months at -80°C and 1 month at -20°C.
Sigma Aldrich Fine Chemicals Biosciences D Luciferin sodium salt ly50MG
D-Luciferin is mainly produced in bioluminescent organisms. It can simply circulate across the blood testis barrier blood brain barrier and the blood placenta barrier. This small molecule is sensitive to light and oxygen. D-Luciferin is also sensitive to moisture in its powder form.
Enzo Life Sciences Z-YVAD-AFC (1mg). CAS: 201608-13-1
Caspase-1 substrate. Fluorogenic substrate for caspase-1. AFC has an excitation maximum of 400nm and an emission maximum of 505nm. This product is similar to Ac-YVAD-AMC, ALX-260-024, but the AFC fluorophore has a greater Stokes' shift upon cleavage and the N-terminal is blocked by a benzyloxycarbonyl moiety, which increases cell permeability. Alternative name: Caspase 1 substrate (fluorogenic). Formula: C39H40N5O11F3. MW: 811.8. Purity: ≥95% (HPLC). Single spot (TLC). Solubility: Soluble in DMSO, DMF or methanol; insoluble in water. Long Term Storage: -20°C. Use/Stability: DMF or DMSO stock solution highly stable at -20°C. Handling: Protect from light.
Cayman Chemical Gly-Gly-AMC 5mg
A fluorogenic peptide substrate; used to assess the activity of bacterial proteases from P. aeruginosa and S. aureus; enzyme activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm
Enzo Life Sciences Z-VVR-AMC (10mg). CAS: 124485-41-2
Sensitive fluorogenic substrate for cathepsin S and cathepsin L. Also cleaved by cathepsin B, cathepsin V, kallikrein 8, and factor VIIa/TF but not cathepsin K nor kallikrein 2. Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450. This substrate is useful for inhibitor screening and kinetic analysis. Also available: fluorogenic calibration standard AMC (BML-KI144). Alternative name: Cathepsin S & L substrate (fluorogenic). Purity: ≥99% (TLC). Solubility: Soluble in DMSO. Long Term Storage: -20°C.
Medchemexpress LLC Boc-Lys(Ac)-AMC | 233691-67-3 | 98.6% | 445.51 | 25 MG
Boc-Lys(Ac)-AMC is a cell-permeable fluorometric HDAC substrate with excitation/emission wavelengths of 355 nm/460 nm.
- Cell-permeable fluorometric HDAC substrate
- Excitation/Emission = 355 nm/460 nm
Cayman Chemical mP-3 FluorogenIc Substrate 1mg
A fluorogenic substrate for MMP-3; Mca is released upon cleavage by MMP-3 and its fluorescence can be used to quantify MMP-3 activity
Medchemexpress LLC HY-52112 100mg Medchemexpress, BCDA CAS:3252-36-6 Purity:>98%
Medchemexpress, HY-52112 100mg BCDA CAS:3252-36-6 BCDA (5-bromo-4-chloroindoxyl acetate) is a chromogenic substrate of esterase used to potently detect the activity of esterase. Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Abcam Nitrotetrazolium blue chloride (NBT), Chromogenic substrate, 250MG
MW 817.64 g/mol. Chromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Boc-Lys(Ac)-AMC | 233691-67-3 | MFCD02683946 | 98.59% | 445.51 | 50 MG
Boc-Lys(Ac)-AMC is a cell-permeable fluorometric substrate for histone deacetylases (HDACs). Following deacetylation by an HDAC, Boc-Lys-AMC is cleaved by trypsin, releasing 7-amino-4-methylcoumarin (AMC), whose fluorescence (Ex/Em = 355 nm/460 nm) can be used to quantify HDAC activity.
- Cell-permeable fluorometric HDAC substrate
- Quantifies histone deacetylase activity
- Fluorescence excitation/emission at 355 nm/460 nm
- For research use only
Medchemexpress LLC Recombinant canine HER2 (CD340) protein, C-terminal His-tag | >95.0% | 10 UG
Recombinant canine HER2 (CD340) protein representing the extracellular region (T23-T652), produced in HEK293 mammalian cells and supplied with a C-terminal His tag. The product is provided lyophilized for reconstitution and is intended for use in biochemical assays, antibody characterization, immunoassays, and binding studies requiring the canine ERBB2 ectodomain.
- Expression in HEK293 mammalian cells for proper folding and glycosylation.
- C-terminal His tag for purification and detection.
- Includes amino acid range T23-T652 for extracellular domain studies.
- High purity (>95.0%) as determined by reducing SDS-PAGE.
- Available in small pack sizes including 10 μg for low-volume experiments.
- Reconstitution recommended to ≥100 μg/mL; store at -20°C for long-term stability.