Substrates
Neogen Diagnostics K-Blue Aqueous TMB Substrate, 1 L, Microwell, HRP, Colorimetric,solvent-free
K-Blue Aqueous is a revolutionary 100% solvent-free, non-volatile TMB substrate. It contains 3,3,5,5 tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2) in a one-bottle format with long-term stability and high activity. K-Blue Aqueous develops a deep blue color in the presence of peroxidase labelled conjugate and is ideal for end users working under strict regulatory requirements. The formula is compliant with Annex XVII for REACH and contains no hazardous substances at or above published reporting levels. K-Blue Aqueous has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate. Stable for a minimum of 36-months when stored at 2-8C.
Neogen Diagnostics Enhanced K-Blue TMB Substrate, 1 L, Microwell, HRP, Colorimetric
Enhanced K-Blue is a one bottle stabilized chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3,5,5 tetramethylbenzidine (TMB) and hydrogen peroxide (H2O2). Enhanced K-Blue develops a deep blue color in the presence of peroxidase labelled conjugate. Enhanced K-Blue has been optimized for use with microwell applications, especially ELISA. This substrate is not applicable for use with assays requiring a precipitating substrate. Stable for a minimum of 48 months when stored at 2-8C.
Biotium Chromogenic beta-galactosidase Substrate sampler kit
For researchers wishing to investigate the suitability of our various chromogenic -galactosidase substrates, this sampler kit provides 5 mg each of the following five different substrates: X-Gal, Red-D-Gal, Rose--D-Gal, Purple--D-Gal, and Green--D-Gal.
Zymo Research Corporation 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-GAL) (5 ml) ^ '*'
Ready-to-use sterile 2% X-GAL solution that can be added directly to your agar plates. The X-GAL solution has been uniquely formulated to reduce false positives during blue-white screening and generates a rich blue color that is easily distinguishable from background.
Biotium Aquaphile™ Coelenterazine h, lyophilized solid
Aquaphile coelenterazine native is a bioluminescence substrate in a special formulation format that is readily dissolved in water or PBS buffer for in vivo use.Properties: Yellow solid soluble in water or PBS buffer; Store at -20°C and protect from light; C26H21N3O3; MW: 423; [55779-48-1].
Biotium 10-Acetyl-3, 7-dihydroxyphenoxazine
10-Acetyl-3,7-dihydroxyphenoxazine (N-Acetyl-3,7-dihydroxyphenoxazine or ADHP), marketed under the name Amplex Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H2O2 (reacting in a 1:1 stoichiometry) in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. H2O2 is produced by many enzymatic reactions, and therefore ADHP can be used to detect the activity of many different enzymes. It can be used to detect HRP in immunoassays or detect trace amounts of hydrogen peroxide (H2O2) using fluorometer or microplate reader.Properties: Ex/Em (pH 9)= 571/585 nm (end product); e= 54,000 (end product); White to off-white solid soluble in DMSO or DMF; Store desiccated at -20°C and protect from light; C14H11CNO4; MW: 257.24; [119171-73-2]Amplex Red is a registered trademark of Thermo Fisher Scientific.
Research Products International Corp IPTG, 500 G
IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.
Dioxane-Free
Water Content: <0.49%
Apexbio Technology LLC D-Luciferin sodium salt(Synonyms: Sodium D-Luciferin, Luciferin sodium salt, Luciferase substrate), 10mg, CAS: 103404-75-7.
D-Luciferin sodium salt (CAS 103404-75-7) is a bioluminescent substrate widely utilized in biomedical research and diagnostic applications Upon entering cells D-Luciferin undergoes enzymatic oxidation catalyzed by luciferase enzymes a reaction requiring ATP and molecular oxygen that emits detectable light This ATP-dependent bioluminescent reaction enables non-invasive monitoring of intracellular metabolic status and energy availability Commonly employed in bioluminescence imaging D-Luciferin sodium salt serves as a powerful tool to assess cellular viability metabolism and gene expression in research investigating cell biology oncology and metabolism
Avanti Polar Lipids PIP2 POWDER
L- alpha -Phosphatidylinositol-4,5-bisphosphate (Brain, Porcine-Triammonium Salt). 383907-42-4
Cayman Chemical LIpase Substrate 1.2mL
This assay kit provides the convenience of an all-inclusive cell-based reporter assay system for M-CSFR signaling. In addition to Reporter Cells included are an optimized culture medium for use in reviving the cryopreserved cells a culture medium for use in preparing test sample treatments the physiological activator M-CSF as a positive control Luciferase Detection Reagents and a cell culture-ready assay plate
Cayman Chemical PGE2 Substrate 250uL
A PDGFR and FGFR inhibitor (IC50s 5.35 4.6 28 28 and 78 nM for PDGFRa PDGFRB FGFR1 -2 and -3 respectively) selective for these kinases over FGFR4 (IC50 >1000 nM) but also inhibits c-Kit VEGFR2 VEGFR1 and IGF-1R (IC50s 67 33 36 and 75 nM respectively) as well as EGFR BTK Cdk4/cyclin D3 and MET (IC50s 128 198 214 and 396 nM respectively) inhibits the proliferation of MG-63 U2OS MNNG/HOS and Saos-2 cells (IC50s 0.84 0.76 1.36 and 0.72 UM respectively) inhibits the migration and invasion of U87MG and LN-229 cells at 0.3 and 0.5 UM decreases tumor volume and increases survival in a U87MG orthotopic mouse xenograft model 30 mg/kg
Ubpbio K63-linked tetra-Ubiquitin (human), enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.
Ubpbio K48-linked di-Ubiquitin (human), enzymatically synthesized
Ub chains are formed by conjugating the C-terminal glycine residue of Ub onto any of seven internal lysine residues or the amino group of the previous Ub. Ub chains are classified by the lysine residue used to link Ubs; different Ub chain topologies can result in different signals. For instance, Ub chains linked through lysine 6, 11, 27, 29, 33 and 48 are capable of targeting proteins for proteasomal degradation; in contrast, Ub chains linked through lysine 63 or the N-terminal amino group (linear Ub chains) often play important nonproteolytic functions including regulation of kinase activation and protein translation. All Ub chain products are produced by using of human wild type Ub reacting with specific E2s.