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Biotin-binding protein conjugates for biotinylated antibody probe detection in ELISA and western blot protocols; products may include peroxidase and poly-HRP conjugates, high-intensity fluorescent conjugates, and infrared conjugates.
Thermo Scientific™ CaptureSelect™ Biotin Anti-Factor X Conjugate consists of a 13-kDa recombinant single domain anti-Factor X antibody fragment chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the antibody fragment.
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The In Vitro Biotinylation Kit (with BirA Tagless Enzyme) is a kit designed for in vitro addition of biotin to biotin-acceptor peptides using BirA. This kit comes in a convenient, easy-to-use format and contains all the components necessary to label biotin-acceptor peptides with biotin. The BirA protein provided is a tagless recombinant biotin protein ligase (EC6.3.4.15). It efficiently catalyzes the specific attachment of biotin to the AviTag™. It is a robust reaction in a wide range of conditions, such as 20-40 µM of protein substrate (biotin-acceptor peptide) concentrations and can tolerate up to 1M NaCl and 20% glycerol in the reaction.
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This product has been developed to facilitate conjugating gold nanoparticles to molecules that cannot be conjugated via the classic direct adsorption method. The product is especially suited for covalent conjugation of small ligands. Aurion Gold Nanoparticles (Carboxyl Functionalized) are polyethylene glycol-carboxyl stabilized gold nanoparticle solutions based on defined particle sizes of 6, 10, 15 and 25nm. Particle size: 25 nm
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Immunogen: A peptide from the C-terminal region of human GFAP citrullinated at R416 • Host: Mouse • Species Reactivity: (+) Human; other species not tested • Cross Reactivity: (+) Citrullinated GFAP (-) Native GFAP, other citrullinated proteins • Applications: ELISA, IHC, WB • MW: 37 kDa
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