Cell Function Reagents and Kits
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Filtered Search Results
Biotium AQUAPHILE JC-1 DMSO
AQUAPHILE JC-1 DMSO
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Biotium MAG-FLUO4 AM ESTER 10X50UG
MAG-FLUO4 AM ESTER 10X50UG
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Medchemexpress LLC HY-15621A 5mg Medchemexpress, DMA (trihydrochloride) CAS:2095832-33-8 Purity:>98%
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Medchemexpress, HY-15621A 5mg DMA (trihydrochloride) CAS:2095832-33-8 DMA trihydrochloride is a fluorescent compound (λex=340 nm, λem=478 nm). Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
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Biotium PMA PCR VIABILITY S. AUREUS NU
PMA PCR VIABILITY S. AUREUS NU
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Biotium 56-CFDA SE 25MG
56-CFDA SE 25MG
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Dojindo Molecular Technologies Inc Glucose Assay Kit-WST (200 TESTS) is used to determine intracellular levels of Glucose.
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Glucose, the most important biological energy source, is a known marker for energy metabolism. It is used not only as a marker for diabetes and obesity, but also for monitoring changes in intracellular metabolism such as lactate production. In addition, a recent study suggested that inhibition of the enzymatic activities involved in both glucose and lipid metabolism effectively inhibits cancer cell proliferation.Glucose Assay Kit-WST enables quantitation of glucose as a substrate in energy metabolism. This kit has been optimized to quantify glucose in cell culture supernatants by measuring absorbance in a colorimetric WST reaction. This kit contains a glucose standard solution that can be used to create a standard curve, it is formatted for 96-well microplates and the assay has a detection limit of 0.02 mmol/L glucose.
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Biotium BCECF, AM Ester, 10x100UG
BCECF, AM ester is membrane-permeant and thus can be loaded into cells via incubation. This product is a mixture of three molecular species, all of which are readily hydrolyzed by endogenous esterases into a single BCECF free acid form once they are in the cells. Properties: White solid soluble in DMSO and DMF; Store at -20°C and protect from light, especially in solution; MW: approx. 615; [117464-70-7]
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Biotium Furaptra, Tetrasodium Salt
Mag-Fura-2 is a UV-excitable fluorescent indicator for magnesium with a Kd of 1.9 mM. Similar to Fura-2, the excitation wavelength of Mag-Fura-2 undergoes a blue shift from 369 nm to 330 nm. Mag-Fura-2 also responds to Ca2 but with a significantly lower Kd than Fura-2 for Ca2 . An important application of Mag-Fura-2 is its use in detecting high, transient Ca2 concentration during Ca2 spikes. Mag-Fura-2/tetrasodium salt can be loaded into cells by microinjection or scrape loading. Also available are Mag-Fura-2, tetrapotassium salt and the cell membrane-permeant Mag-Fura-2 AM ester. Properties: Light yellow solid soluble in water (pH > 6); Store at 4°C and protect from light C18H10N2Na4O11; MW: 523
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Biotium 5-CFDA (5-Carboxyfluorescein Diacetate,
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein. Properties: Off-white solid soluble in DMSO; Store desiccated at 4°C; C25H16O9; MW: 460; [79955-27-4].
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Abcam LIVE AND DEAD CELL STAINING KI ..
LIVE AND DEAD CELL STAINING KI ..
The product is subject to the following: Abcam Restricted Use Statement
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Sigma Aldrich Fine Chemicals Biosciences Live Dead Cell Viability A
The Live-Dead Cell Viability Assay Kit is a quick and simple three-color assay to measure cell viability. The kit consists of Calcein-AM (stains live cells) Propidium Iodide (stains dead cells) and Hoechst 33342 (stains all cells). The kit has been optimized for 3D cell culture (spheroids human organoids and 3D matrices) and 2D cell culture (multiple cell types). The kit can be used for flow cytometry fluorescence microscopy and with fluorescence microplate readers. The kit determines viability of cells based on plasma membrane integrity and esterase activity. One kit contains reagents sufficient for 5 x 24-well plates or 12 x 96-well plates.boldAdvantages/bold 1. Tested and optimized for 3D cell culture Spheroid culture organoid culture 2D cell culture and flow cytometry.2. Easy to use and quick protocol.3. Stronger intensity of Calcein AM and Hoechst dyes compared to other products.Spectral PropertiesCalcein AM Ex/Em 490/515 nm (Green)Propidium Iodide Ex/Em 535/617 nm (Red)
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Biotium Fura-2, AM Ester, 1mM In Anhydrous DMSO
Fura-2, AM ester is an intracellular fluorescent calcium indicator, and the preferred dye for ratiometric imaging microscopy. The membrane-permeant AM ester can be loaded into cells via incubation, but due to limited water solubility, a mild detergent Pluronic F-127 is often used to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2, but intracellular esterases readily hydrolyze it to Fura-2. Upon calcium binding, the excitation maximum of Fura-2 undergoes a blue-shift from 363 nm (no Ca2) to 335 nm (high Ca2) while the emission maximum is relatively unchanged. Measure by flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C44H47N3O24; MW: 1001.9; [108964-32-5].
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Biotium Resazurin, sodium salt
Resazurin is the N-oxide of the fluorescent dye resorufin and used to detect reductive activities in cells. It is widely used for measuring cell proliferation and mitochondrial metabolic activity. Resazurin itself is non-fluorescent until reduced to the highly red fluorescent resorufin (Ex/Em: 563/587 nm) in the presence of reductants like NADPH or NADH and the enzyme diaphorase. Resazurin can therefore be used to detect NADH, NADPH, or diaphorase levels. Furthermore, the resazurin /diaphorase /NADPH system can be used to detect any biochemical or enzyme activity involved in generating NADH or NADPH. Although resazurin is available from several suppliers, trace amounts of the resorufin contaminant often make the material unsuitable for bioassay applications. This high-grade resazurin has a minimal background fluorescence. Properties: Ex/Em (pH 9) = 604 nm/none; Ex/Em (reduced): 563/587 nm; Dark solid soluble in DMSO or water; Store at 4°C protected from light; C12H6NNaO4; MW: 251.17
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Biotium Fura-2, AM Ester
Fura-2, AM ester is an intracellular fluorescent calcium indicator, and the preferred dye for ratiometric imaging microscopy. The membrane-permeant AM ester can be loaded into cells via incubation, but due to limited water solubility, a mild detergent Pluronic F-127 is often used to facilitate cell loading. Fura-2, AM ester itself does not bind Ca2, but intracellular esterases readily hydrolyze it to Fura-2. Upon calcium binding, the excitation maximum of Fura-2 undergoes a blue-shift from 363 nm (no Ca2) to 335 nm (high Ca2) while the emission maximum is relatively unchanged. Measure by flow cytometry, fluorescence microscopy, or certain fluoresence microplate readers. Properties: Light yellow solid soluble in DMSO; Store at -20°C, protect from light, especially in solution; Kd: 145 nM (22°C, pH 7.2 buffer); Ex/Em = 363/512 nm, e = 27,000 M-1cm-1 (no Ca2); Ex/Em 335/505 nm, e = 35,000 M-1cm-1 (high Ca2); C44H47N3O24; MW: 1001.9; [108964-32-5].
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Biotium Sulforhodamine 101
Sulforhodamine 101 has a longer absorption wavelength than does sulforhodamine B. Like sulforhodamine B and G, in addition to their potential use in cancer drug screening, these fluorescent dyes have been primarily used as polar tracers for the studies of neuronal cell morphology and cell-cell communication. Properties: Ex/Em = 586/605 nm; Red solid soluble in water; Store at room temperature; C31H30N2O7S2; MW: 607; [60311-02-6]
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