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The purpose of this kit is to provide the necessary reagents for the preparation of single cell WTA, Sample Tag, and BD™ AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system.
mutaFISH™ DNA Accessory Kit 1 for Cells provides reagents and enzymes for in situ restriction digestion, exonucleolysis, mutaFISH™ hybridization, ligation and amplification in cell prior to mutaFISH™.
mutaFISH™ DNA Accessory Kit 2 for Cells provides reagents and enzymes for in situ restriction digestion, exonucleolysis, mutaFISH™ hybridization, ligation and amplification in cell prior to mutaFISH™.
The kit can be used with the BD Rhapsody™ Targeted Assay, BD Rhapsody™ Whole Transcriptome Analysis Amplification Kit, BD Rhapsody™ TCR/BCR Profiling Assays, BD™ Single-Cell Multiplexing Kit, and BD™ AbSeq Assays.
The purpose of this kit is to provide the necessary reagents for the preparation of single cell mRNA, Sample Tag, and BD™ AbSeq libraries with the BD Rhapsody™ Single-Cell Analysis system or the BD Rhapsody™ Express Single-Cell Analysis system.
This panel contains primer pairs that target 389 genes commonly expressed in human breast cancer samples that contain breast cancer cells, tumor infiltrating immune cells, and other cell types present in tumor microenvironments.
Uses a DNA-specific dye so there is no need to use RNase.The dye crosses membranes so there is no need to permeabilize or fix the cells.This also means you can use live cells.No wash needed simply add and read for fast easily automated workflow.PrincipleCells stained with the cell cycle dye will fluoresce in proportion to their DNA content. The cell cycle consists of four distinct phases: G0/G1 phase growth S phase synthesis G2 phase and M phase mitosis . Each phase can be identified based on the relative amounts of DNA resident in the cell with G0/G1 cells having the least amount of DNA and G2/M cells with the most. These relative differences in DNA content are easily detected by fluorescence intensity and can be utilized to establish quantitative cell cycle profiles.Multiplex with other iQue kits like Cell Membrane Integrity for richer content.
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